hERG stereoselective modulation by mexiletine-derived ureas: Molecular docking study, synthesis, and biological evaluation.

Long QT syndrome (LQTS) is a disorder of cardiac electrophysiology resulting in life-threatening arrhythmias; nowadays, only a few drugs are available for the management of LQTS. Focusing our attention on LQT2, one of the most common subtypes of LQTS caused by mutations in the human ether-à-go-go-re...

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Detalles Bibliográficos
Autores: Milani, Gualtiero, Budriesi, Roberta, Tavazzani, Elisa, Cavalluzzi, Maria Maddalena, Mattioli, Laura Beatrice, Miniero, Daniela Valeria, Delre, Pietro, Belviso, Benny Danilo, Denegri, Marco, Cuocci, Corrado, Rotondo, Natalie Paola, De Palma, Annalisa, Gualdani, Roberta, Caliandro, Rocco, Mangiatordi, Giuseppe Felice, Kumawat, Amit, Camilloni, Carlo, Priori, Silvia G., Lentini, Giovanni
Tipo de recurso: artículo
Fecha de publicación:2023
País:España
Institución:Instituto de Salud Carlos III (ISCIII)
Repositorio:Repisalud
Idioma:inglés
OAI Identifier:oai:repisalud.isciii.es:20.500.12105/16568
Acceso en línea:http://hdl.handle.net/20.500.12105/16568
Access Level:acceso abierto
Palabra clave:Mexiletine
Long QT Syndrome
Humans
Animals
Guinea Pigs
Molecular Docking Simulation
Urea
Structure-Activity Relationship
Potassium Channels
Descripción
Sumario:Long QT syndrome (LQTS) is a disorder of cardiac electrophysiology resulting in life-threatening arrhythmias; nowadays, only a few drugs are available for the management of LQTS. Focusing our attention on LQT2, one of the most common subtypes of LQTS caused by mutations in the human ether-à-go-go-related gene (hERG), in the present work, the stereoselectivity of the recently discovered mexiletine-derived urea 8 was investigated on the hERG potassium channel. According to preliminary in silico predictions, in vitro studies revealed a stereoselective behavior, with the meso form showing the greatest hERG opening activity. In addition, functional studies on guinea pig isolated left atria, aorta, and ileum demonstrated that 8 does not present any cardiac or intestinal liability in our ex vivo studies. Due to its overall profile, (R,S)-8 paves the way for the design and development of a new series of compounds potentially useful in the treatment of both congenital and drug-induced forms of LQTS.