Assessing the enzymatic effects of cellulases and LPMO in improving mechanical fibrillation of cotton linters

Background: The increasing interest in replacing petroleum-based products by more sustainable materials in the packaging sector gives relevance to cellulose as a biodegradable natural resource. Moreover, its properties can be modified physically, chemically or biotechnologically in order to obtain n...

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Autores: Valls Vidal, Cristina|||0000-0003-2307-1779, Pastor Blasco, Francisco Ignacio Javier, Roncero Vivero, María Blanca|||0000-0002-2694-2368, Vidal Lluciá, Teresa|||0000-0001-6269-4114, Diaz Lucea, M. Pilar, Martinez Soler, Josefina, Valenzuela, Susana V.
Tipo de recurso: artículo
Fecha de publicación:2019
País:España
Institución:Universitat Politècnica de Catalunya (UPC)
Repositorio:UPCommons. Portal del coneixement obert de la UPC
Idioma:inglés
OAI Identifier:oai:upcommons.upc.edu:2117/168369
Acceso en línea:https://hdl.handle.net/2117/168369
https://dx.doi.org/10.1186/s13068-019-1502-z
Access Level:acceso abierto
Palabra clave:Cellulose--Biotechnology
Cellulose
Cotton linters
LPMO
Laccase_Tempo
Cellulases
NFC
Cel·lulosa -- Biotecnologia
Cel·lulasa
Àrees temàtiques de la UPC::Enginyeria paperera::Primeres matèries papereres::Cel·lulosa
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spelling Assessing the enzymatic effects of cellulases and LPMO in improving mechanical fibrillation of cotton lintersValls Vidal, Cristina|||0000-0003-2307-1779Pastor Blasco, Francisco Ignacio JavierRoncero Vivero, María Blanca|||0000-0002-2694-2368Vidal Lluciá, Teresa|||0000-0001-6269-4114Diaz Lucea, M. PilarMartinez Soler, JosefinaValenzuela, Susana V.Cellulose--BiotechnologyCelluloseCotton lintersLPMOLaccase_TempoCellulasesNFCCel·lulosa -- BiotecnologiaCel·lulasaÀrees temàtiques de la UPC::Enginyeria paperera::Primeres matèries papereres::Cel·lulosaBackground: The increasing interest in replacing petroleum-based products by more sustainable materials in the packaging sector gives relevance to cellulose as a biodegradable natural resource. Moreover, its properties can be modified physically, chemically or biotechnologically in order to obtain new bioproducts. Refined cotton linters with high cellulose content were treated with hydrolytic (cellulases) and oxidative (LPMO and Laccase_Tempo) enzymes to evaluate their effect on fibre properties and in improving mechanical fibrillation. Results: Cellulases released cellooligosaccharides, reducing fibre length and partially degrading cellulose. They also improved mechanical fibrillation yielding up to 18% of nanofibrillated cellulose (NFC). LPMO introduced a slight amount of COOH groups in cellulose fibres, releasing cellobionic acid to the effluents. The action of cellulases was improved after LPMO treatment; however, the COOH groups created disappeared from fibres. After mechanical fibrillation of LPMO–cellulase-treated cotton linters a 23% yield of NFC was obtained. Laccase_Tempo treatment also introduced COOH groups in cellulose fibres from cotton, yielding 10% of NFC. Degree of polymerization was reduced by Laccase_Tempo, while LPMO treatment did not significantly affect it but produced a higher reduction in fibre length. The combined treatment with LPMO and cellulase provided films with higher transparency (86%), crystallinity (92%), smoothness and improved barrier properties to air and water than films casted from non-treated linters and from commercial NFC. Conclusions: The combined enzymatic treatment with LPMO and cellulases boosted mechanical fibrillation of cotton linters, improving the NFC production and providing bioproducts with high transparency and high barrier properties20192019-06-2620192019-09-18journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/2117/168369https://dx.doi.org/10.1186/s13068-019-1502-zreponame:UPCommons. Portal del coneixement obert de la UPCinstname:Universitat Politècnica de Catalunya (UPC)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution 3.0 Spainhttp://creativecommons.org/licenses/by/3.0/es/info:eu-repo/semantics/openAccessoai:upcommons.upc.edu:2117/1683692026-05-27T15:37:01Z
dc.title.none.fl_str_mv Assessing the enzymatic effects of cellulases and LPMO in improving mechanical fibrillation of cotton linters
title Assessing the enzymatic effects of cellulases and LPMO in improving mechanical fibrillation of cotton linters
spellingShingle Assessing the enzymatic effects of cellulases and LPMO in improving mechanical fibrillation of cotton linters
Valls Vidal, Cristina|||0000-0003-2307-1779
Cellulose--Biotechnology
Cellulose
Cotton linters
LPMO
Laccase_Tempo
Cellulases
NFC
Cel·lulosa -- Biotecnologia
Cel·lulasa
Àrees temàtiques de la UPC::Enginyeria paperera::Primeres matèries papereres::Cel·lulosa
title_short Assessing the enzymatic effects of cellulases and LPMO in improving mechanical fibrillation of cotton linters
title_full Assessing the enzymatic effects of cellulases and LPMO in improving mechanical fibrillation of cotton linters
title_fullStr Assessing the enzymatic effects of cellulases and LPMO in improving mechanical fibrillation of cotton linters
title_full_unstemmed Assessing the enzymatic effects of cellulases and LPMO in improving mechanical fibrillation of cotton linters
title_sort Assessing the enzymatic effects of cellulases and LPMO in improving mechanical fibrillation of cotton linters
dc.creator.none.fl_str_mv Valls Vidal, Cristina|||0000-0003-2307-1779
Pastor Blasco, Francisco Ignacio Javier
Roncero Vivero, María Blanca|||0000-0002-2694-2368
Vidal Lluciá, Teresa|||0000-0001-6269-4114
Diaz Lucea, M. Pilar
Martinez Soler, Josefina
Valenzuela, Susana V.
author Valls Vidal, Cristina|||0000-0003-2307-1779
author_facet Valls Vidal, Cristina|||0000-0003-2307-1779
Pastor Blasco, Francisco Ignacio Javier
Roncero Vivero, María Blanca|||0000-0002-2694-2368
Vidal Lluciá, Teresa|||0000-0001-6269-4114
Diaz Lucea, M. Pilar
Martinez Soler, Josefina
Valenzuela, Susana V.
author_role author
author2 Pastor Blasco, Francisco Ignacio Javier
Roncero Vivero, María Blanca|||0000-0002-2694-2368
Vidal Lluciá, Teresa|||0000-0001-6269-4114
Diaz Lucea, M. Pilar
Martinez Soler, Josefina
Valenzuela, Susana V.
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Cellulose--Biotechnology
Cellulose
Cotton linters
LPMO
Laccase_Tempo
Cellulases
NFC
Cel·lulosa -- Biotecnologia
Cel·lulasa
Àrees temàtiques de la UPC::Enginyeria paperera::Primeres matèries papereres::Cel·lulosa
topic Cellulose--Biotechnology
Cellulose
Cotton linters
LPMO
Laccase_Tempo
Cellulases
NFC
Cel·lulosa -- Biotecnologia
Cel·lulasa
Àrees temàtiques de la UPC::Enginyeria paperera::Primeres matèries papereres::Cel·lulosa
description Background: The increasing interest in replacing petroleum-based products by more sustainable materials in the packaging sector gives relevance to cellulose as a biodegradable natural resource. Moreover, its properties can be modified physically, chemically or biotechnologically in order to obtain new bioproducts. Refined cotton linters with high cellulose content were treated with hydrolytic (cellulases) and oxidative (LPMO and Laccase_Tempo) enzymes to evaluate their effect on fibre properties and in improving mechanical fibrillation. Results: Cellulases released cellooligosaccharides, reducing fibre length and partially degrading cellulose. They also improved mechanical fibrillation yielding up to 18% of nanofibrillated cellulose (NFC). LPMO introduced a slight amount of COOH groups in cellulose fibres, releasing cellobionic acid to the effluents. The action of cellulases was improved after LPMO treatment; however, the COOH groups created disappeared from fibres. After mechanical fibrillation of LPMO–cellulase-treated cotton linters a 23% yield of NFC was obtained. Laccase_Tempo treatment also introduced COOH groups in cellulose fibres from cotton, yielding 10% of NFC. Degree of polymerization was reduced by Laccase_Tempo, while LPMO treatment did not significantly affect it but produced a higher reduction in fibre length. The combined treatment with LPMO and cellulase provided films with higher transparency (86%), crystallinity (92%), smoothness and improved barrier properties to air and water than films casted from non-treated linters and from commercial NFC. Conclusions: The combined enzymatic treatment with LPMO and cellulases boosted mechanical fibrillation of cotton linters, improving the NFC production and providing bioproducts with high transparency and high barrier properties
publishDate 2019
dc.date.none.fl_str_mv 2019
2019-06-26
2019
2019-09-18
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/2117/168369
https://dx.doi.org/10.1186/s13068-019-1502-z
url https://hdl.handle.net/2117/168369
https://dx.doi.org/10.1186/s13068-019-1502-z
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 3.0 Spain
http://creativecommons.org/licenses/by/3.0/es/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 3.0 Spain
http://creativecommons.org/licenses/by/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:UPCommons. Portal del coneixement obert de la UPC
instname:Universitat Politècnica de Catalunya (UPC)
instname_str Universitat Politècnica de Catalunya (UPC)
reponame_str UPCommons. Portal del coneixement obert de la UPC
collection UPCommons. Portal del coneixement obert de la UPC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300724

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