Increasing yield of nanocrystalline cellulose preparation process by a cellulase pretreatment
In this work the introduction of a cellulase treatment prior to NCC isolation was assessed. NCC was produced using sulfuric acid at two different concentrations (62 and 64% wt.). The effect of pore size for filtration step was also assessed. The smaller acid dose leaded to yields up to 65–70% and av...
| Autores: | , , , , |
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| Tipo de recurso: | artículo |
| Fecha de publicación: | 2015 |
| País: | España |
| Institución: | Universitat Politècnica de Catalunya (UPC) |
| Repositorio: | UPCommons. Portal del coneixement obert de la UPC |
| Idioma: | inglés |
| OAI Identifier: | oai:upcommons.upc.edu:2117/28571 |
| Acceso en línea: | https://hdl.handle.net/2117/28571 https://dx.doi.org/10.1016/j.biortech.2015.06.007 |
| Access Level: | acceso abierto |
| Palabra clave: | Cellulose--Chemistry Cellulase Nanocrystalline cellulose Enzymatic treatment Yield increase Acid hydrolysis Cel·lulosa microcristal·lina Cel·lulasa Àrees temàtiques de la UPC::Enginyeria paperera::Primeres matèries papereres::Cel·lulosa |
| Sumario: | In this work the introduction of a cellulase treatment prior to NCC isolation was assessed. NCC was produced using sulfuric acid at two different concentrations (62 and 64% wt.). The effect of pore size for filtration step was also assessed. The smaller acid dose leaded to yields up to 65–70% and average size up to 160 nm. It also produced crystals with reduced sulfur content (0.6–1%). Cellulase pretreatment influenced NCC characteristics, as it increased overall yield a 12%, increased average particle size around 35 nm and reduced NCC sulfur content up to a 0.8%. We found that different conditions of enzymatic treatments led to quantitative differences on their effects on NCC. Acetate buffer used for enzymatic treatments was found to counteract effects of acid. The evidence presented in this work suggested that pretreating fibers with this cellulase represents a very interesting option to partially replace chemicals on NCC isolation |
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