Characterization In vitro and In vivo of a pandemic H1N1 influenza virus from a fatal case

Pandemic 2009 H1N1 (pH1N1) influenza viruses caused mild symptoms in most infected patients. However, a greater rate of severe disease was observed in healthy young adults and children without co-morbid conditions. Here we tested whether influenza strains displaying differential virulence could be p...

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Detalhes bibliográficos
Autores: Rodriguez, Ariel, Falcón, Ana María, Cuevas, María Teresa, Pozo, Francisco, Guerra García, María Susana, García-Barreno, Blanca, Martínez-Orellana, Pamela, Pérez-Breña, Pilar, Montoya, María, Melero, José Antonio, Pizarro, Manuel, Ortín, Juan, Casas, Inmaculada, Nieto, Amelia
Formato: artículo
Fecha de publicación:2013
País:España
Recursos:Universidad Autónoma de Madrid
Repositorio:Biblos-e Archivo. Repositorio Institucional de la UAM
Idioma:inglés
OAI Identifier:oai:repositorio.uam.es:10486/666900
Acesso em linha:http://hdl.handle.net/10486/666900
https://dx.doi.org/10.1371/journal.pone.0053515
Access Level:acceso abierto
Palavra-chave:Amino Acids
Cells
Immunohistochemistry
Influenza
Pandemics
Inbred BALB C
Medicina
Descrição
Resumo:Pandemic 2009 H1N1 (pH1N1) influenza viruses caused mild symptoms in most infected patients. However, a greater rate of severe disease was observed in healthy young adults and children without co-morbid conditions. Here we tested whether influenza strains displaying differential virulence could be present among circulating pH1N1 viruses. The biological properties and the genotype of viruses isolated from a patient showing mild disease (M) or from a fatal case (F), both without known co-morbid conditions were compared in vitro and in vivo. The F virus presented faster growth kinetics and stronger induction of cytokines than M virus in human alveolar lung epithelial cells. In the murine model in vivo, the F virus showed a stronger morbidity and mortality than M virus. Remarkably, a higher proportion of mice presenting infectious virus in the hearts, was found in F virus-infected animals. Altogether, the data indicate that strains of pH1N1 virus with enhanced pathogenicity circulated during the 2009 pandemic. In addition, examination of chemokine receptor 5 (CCR5) genotype, recently reported as involved in severe influenza virus disease, revealed that the F virus-infected patient was homozygous for the deleted form of CCR5 receptor (CCR5Δ32)

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