Fast and high resolution mapping of elastic properties of biomolecules and polymers with bimodal AFM

[EN] Fast, high resolution and wide elastic modulus range mapping of heterogeneous interfaces represents a major goal of atomic force microscopy (AFM). This goal becomes more challenging when the nanomechanical mapping involves biomolecules in their native environment. Over the years, several AFM-ba...

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Autores: Benaglia, Simone, Gisbert, Victor G., Perrino, Alma P., Amo, Carlos A., García García, Ricardo
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2018
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/172908
Acceso en línea:http://hdl.handle.net/10261/172908
Access Level:acceso abierto
Palabra clave:Applications of AFM
Atomic force microscopy
Nanoscale biophysics
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spelling Fast and high resolution mapping of elastic properties of biomolecules and polymers with bimodal AFMBenaglia, SimoneGisbert, Victor G.Perrino, Alma P.Amo, Carlos A.García García, RicardoApplications of AFMAtomic force microscopyNanoscale biophysics[EN] Fast, high resolution and wide elastic modulus range mapping of heterogeneous interfaces represents a major goal of atomic force microscopy (AFM). This goal becomes more challenging when the nanomechanical mapping involves biomolecules in their native environment. Over the years, several AFM-based methods have been developed to address that goal. However, none of those methods combine sub-nanometer spatial resolution, quantitative accuracy, fast data acquisition speed, wide elastic modulus range and operation in physiological solutions. Here we present detailed protocols to generate high resolution maps of the elastic properties of biomolecules and polymers by using bimodal AFM. The method is fast because the elastic modulus, deformation and topography images are obtained simultaneously. The method is efficient because just a single data point per pixel is needed to generate the above images. In addition, by knowing the deformation, bimodal AFM enables to reconstruct the true topography of the surface.European Research Council (ERC–AdG–340177; 3DNanoMech) and grants CSD201000024 and MAT2016-76507-R from the Ministerio de Economía y Competitividad. This work received funding from the European Union’s Horizon 2020 Research and Innovation Programme under Marie Sklodowska-Curie grant agreement 721874 (SPM2.0). We also acknowledge fellowships FPU15/04622 (C.A.A.) and BES-2017-081907 (V.G.G.) from the Ministerio de Educación.Peer reviewedSpringer NatureMinisterio de Economía y Competitividad (España)European Research CouncilEuropean CommissionMinisterio de Educación y Ciencia (España)García García, Ricardo [0000-0002-7115-1928]Perrino, Alma P. [0000-0003-3994-7472]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201820182018info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/172908reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/EC/FP7/340177info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/MAT2016-76507-Rinfo:eu-repo/grantAgreement/EC/H2020/721874https://doi.org/10.1038/s41596-018-0070-1Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1729082026-05-22T06:33:51Z
dc.title.none.fl_str_mv Fast and high resolution mapping of elastic properties of biomolecules and polymers with bimodal AFM
title Fast and high resolution mapping of elastic properties of biomolecules and polymers with bimodal AFM
spellingShingle Fast and high resolution mapping of elastic properties of biomolecules and polymers with bimodal AFM
Benaglia, Simone
Applications of AFM
Atomic force microscopy
Nanoscale biophysics
title_short Fast and high resolution mapping of elastic properties of biomolecules and polymers with bimodal AFM
title_full Fast and high resolution mapping of elastic properties of biomolecules and polymers with bimodal AFM
title_fullStr Fast and high resolution mapping of elastic properties of biomolecules and polymers with bimodal AFM
title_full_unstemmed Fast and high resolution mapping of elastic properties of biomolecules and polymers with bimodal AFM
title_sort Fast and high resolution mapping of elastic properties of biomolecules and polymers with bimodal AFM
dc.creator.none.fl_str_mv Benaglia, Simone
Gisbert, Victor G.
Perrino, Alma P.
Amo, Carlos A.
García García, Ricardo
author Benaglia, Simone
author_facet Benaglia, Simone
Gisbert, Victor G.
Perrino, Alma P.
Amo, Carlos A.
García García, Ricardo
author_role author
author2 Gisbert, Victor G.
Perrino, Alma P.
Amo, Carlos A.
García García, Ricardo
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
European Research Council
European Commission
Ministerio de Educación y Ciencia (España)
García García, Ricardo [0000-0002-7115-1928]
Perrino, Alma P. [0000-0003-3994-7472]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Applications of AFM
Atomic force microscopy
Nanoscale biophysics
topic Applications of AFM
Atomic force microscopy
Nanoscale biophysics
description [EN] Fast, high resolution and wide elastic modulus range mapping of heterogeneous interfaces represents a major goal of atomic force microscopy (AFM). This goal becomes more challenging when the nanomechanical mapping involves biomolecules in their native environment. Over the years, several AFM-based methods have been developed to address that goal. However, none of those methods combine sub-nanometer spatial resolution, quantitative accuracy, fast data acquisition speed, wide elastic modulus range and operation in physiological solutions. Here we present detailed protocols to generate high resolution maps of the elastic properties of biomolecules and polymers by using bimodal AFM. The method is fast because the elastic modulus, deformation and topography images are obtained simultaneously. The method is efficient because just a single data point per pixel is needed to generate the above images. In addition, by knowing the deformation, bimodal AFM enables to reconstruct the true topography of the surface.
publishDate 2018
dc.date.none.fl_str_mv 2018
2018
2018
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/172908
url http://hdl.handle.net/10261/172908
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
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info:eu-repo/grantAgreement/EC/FP7/340177
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/MAT2016-76507-R
info:eu-repo/grantAgreement/EC/H2020/721874
https://doi.org/10.1038/s41596-018-0070-1
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Springer Nature
publisher.none.fl_str_mv Springer Nature
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
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