Synergistic activity of floor-plate- and ventricular-zone-derived Netrin-1 in spinal cord commissural axon guidance

In vertebrates, commissural axons extend ventrally toward the floor plate in the spinal cord and hindbrain. Netrin-1, secreted by floor plate cells, was proposed to attract commissural axons at a distance. However, recent genetic studies in mice have shown that netrin-1 is also produced by ventricul...

Descripción completa

Detalles Bibliográficos
Autores: Moreno-Bravo, Juan Antonio, Roig-Puiggros, Sergi, Mehlen, Patrick, Chédotal, Alain
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2019
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/338247
Acceso en línea:http://hdl.handle.net/10261/338247
Access Level:acceso abierto
id ES_19ac43ec8331cd911eaa378dc67f69e1
oai_identifier_str oai:digital.csic.es:10261/338247
network_acronym_str ES
network_name_str España
repository_id_str
spelling Synergistic activity of floor-plate- and ventricular-zone-derived Netrin-1 in spinal cord commissural axon guidanceMoreno-Bravo, Juan AntonioRoig-Puiggros, SergiMehlen, PatrickChédotal, AlainIn vertebrates, commissural axons extend ventrally toward the floor plate in the spinal cord and hindbrain. Netrin-1, secreted by floor plate cells, was proposed to attract commissural axons at a distance. However, recent genetic studies in mice have shown that netrin-1 is also produced by ventricular zone (VZ) progenitors and that in the hindbrain, it represents the main source of netrin-1 for commissural axons. Here, we show that genetically deleting netrin-1 either from the VZ or the floor plate does not prevent midline crossing in the spinal cord, although axon pathfinding and fasciculation are perturbed. Strikingly, the VZ and floor plate act synergistically, as the simultaneous ablation of netrin-1 from these two sources severely impedes crossing. These results suggest that floor-plate-derived netrin-1 has a distinct impact on commissural axons in the spinal cord and hindbrain.This work was supported by grants from the Agence Nationale de la Recherche (ANR-14-CE13-0004-01) (A.C.) and was performed in the frame of the LABEX LIFESENSES (reference ANR-10-LABX-65), which is supported by French state funds managed by the ANR within the Investissements d’Avenir programme under reference ANR-11-IDEX-0004-02 (A.C.).Peer reviewedElsevierAgence Nationale de la Recherche (France)202320232019info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/338247reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésThe underlying dataset has been published as supplementary material of the article in the publisher platform at DOI 10.1016/j.neuron.2018.12.024Long-Range Guidance of Spinal Commissural Axons by Netrin1 and Sonic Hedgehog from Midline Floor Plate Cells Neuron, Volume 101, Issue 4, 20 February 2019, Pages 635-647.e4 Zhuhao Wu, Shirin Makihara, Patricia T. Yam, Shaun Teo, Nicolas Renier, Nursen Balekoglu, Juan Antonio Moreno-Bravo, Olav Olsen, Alain Chédotal, Frédéric Charron, Marc Tessier-Lavignehttps://doi.org/10.1016/j.neuron.2018.12.024Noinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3382472026-05-22T06:33:51Z
dc.title.none.fl_str_mv Synergistic activity of floor-plate- and ventricular-zone-derived Netrin-1 in spinal cord commissural axon guidance
title Synergistic activity of floor-plate- and ventricular-zone-derived Netrin-1 in spinal cord commissural axon guidance
spellingShingle Synergistic activity of floor-plate- and ventricular-zone-derived Netrin-1 in spinal cord commissural axon guidance
Moreno-Bravo, Juan Antonio
title_short Synergistic activity of floor-plate- and ventricular-zone-derived Netrin-1 in spinal cord commissural axon guidance
title_full Synergistic activity of floor-plate- and ventricular-zone-derived Netrin-1 in spinal cord commissural axon guidance
title_fullStr Synergistic activity of floor-plate- and ventricular-zone-derived Netrin-1 in spinal cord commissural axon guidance
title_full_unstemmed Synergistic activity of floor-plate- and ventricular-zone-derived Netrin-1 in spinal cord commissural axon guidance
title_sort Synergistic activity of floor-plate- and ventricular-zone-derived Netrin-1 in spinal cord commissural axon guidance
dc.creator.none.fl_str_mv Moreno-Bravo, Juan Antonio
Roig-Puiggros, Sergi
Mehlen, Patrick
Chédotal, Alain
author Moreno-Bravo, Juan Antonio
author_facet Moreno-Bravo, Juan Antonio
Roig-Puiggros, Sergi
Mehlen, Patrick
Chédotal, Alain
author_role author
author2 Roig-Puiggros, Sergi
Mehlen, Patrick
Chédotal, Alain
author2_role author
author
author
dc.contributor.none.fl_str_mv Agence Nationale de la Recherche (France)
description In vertebrates, commissural axons extend ventrally toward the floor plate in the spinal cord and hindbrain. Netrin-1, secreted by floor plate cells, was proposed to attract commissural axons at a distance. However, recent genetic studies in mice have shown that netrin-1 is also produced by ventricular zone (VZ) progenitors and that in the hindbrain, it represents the main source of netrin-1 for commissural axons. Here, we show that genetically deleting netrin-1 either from the VZ or the floor plate does not prevent midline crossing in the spinal cord, although axon pathfinding and fasciculation are perturbed. Strikingly, the VZ and floor plate act synergistically, as the simultaneous ablation of netrin-1 from these two sources severely impedes crossing. These results suggest that floor-plate-derived netrin-1 has a distinct impact on commissural axons in the spinal cord and hindbrain.
publishDate 2019
dc.date.none.fl_str_mv 2019
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/338247
url http://hdl.handle.net/10261/338247
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI 10.1016/j.neuron.2018.12.024
Long-Range Guidance of Spinal Commissural Axons by Netrin1 and Sonic Hedgehog from Midline Floor Plate Cells Neuron, Volume 101, Issue 4, 20 February 2019, Pages 635-647.e4 Zhuhao Wu, Shirin Makihara, Patricia T. Yam, Shaun Teo, Nicolas Renier, Nursen Balekoglu, Juan Antonio Moreno-Bravo, Olav Olsen, Alain Chédotal, Frédéric Charron, Marc Tessier-Lavigne
https://doi.org/10.1016/j.neuron.2018.12.024
No
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869404059719434240
score 15.812429

Similares en LA Referencia