Loss of floor plate Netrin-1 impairs midline crossing of corticospinal axons and leads to mirror movements

In humans, execution of unimanual movements requires lateralized activation of the primary motor cortex, which then transmits the motor command to the contralateral hand through the crossed corticospinal tract (CST). Mutations in NTN1 alter motor control lateralization, leading to congenital mirror...

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Detalles Bibliográficos
Autores: Pourchet, Oriane, Morel, Marie-Pierre, Welniarz, Quentin, Sarrazin, Nadège, Marti, Fabio, Heck, Nicolas, Galléa, Cécile, Doulazmi, Mohamed, Roig-Puiggros, Sergi, Moreno-Bravo, Juan Antonio, Vidailhet, Marie, Trembleau, Alain, Faure, Philippe, Chédotal, Alain, Roze, Emmanuel, Dusart, Isabelle
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/308989
Acceso en línea:http://hdl.handle.net/10261/308989
Access Level:acceso abierto
Palabra clave:Brain development
Motor control
Neural circuit development
Descripción
Sumario:In humans, execution of unimanual movements requires lateralized activation of the primary motor cortex, which then transmits the motor command to the contralateral hand through the crossed corticospinal tract (CST). Mutations in NTN1 alter motor control lateralization, leading to congenital mirror movements. To address the role of midline Netrin-1 on CST development and subsequent motor control, we analyze the morphological and functional consequences of floor plate Netrin-1 depletion in conditional knockout mice. We show that depletion of floor plate Netrin-1 in the brainstem critically disrupts CST midline crossing, whereas the other commissural systems are preserved. The only associated defect is an abnormal entry of CST axons within the inferior olive. Alteration of CST midline crossing results in functional ipsilateral projections and is associated with abnormal symmetric movements. Our study reveals the role of Netrin-1 in CST development and describes a mouse model recapitulating the characteristics of human congenital mirror movements.