Respuesta del sistema radicular frente a la deficiencia de fósforo en el contexto de plantas injertadas, análisis fenotípico y de expresión génica

Disproportionate use of fertilizers has environmental issues derivate from soil and water bodies accumulation, as well as economic repercussions because of the increased price of agricultural inputs due to the limited availability of raw materials, is the case of phosphate rock. One of the most impo...

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Detalhes bibliográficos
Autor: Gil Villar, Daniel
Formato: tesis de maestría
Fecha de publicación:2017
País:España
Recursos:Universitat Politècnica de València (UPV)
Repositorio:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
Idioma:español
OAI Identifier:oai:riunet.upv.es:10251/92602
Acesso em linha:https://riunet.upv.es/handle/10251/92602
Access Level:acceso abierto
Palavra-chave:Capsicum annuum
Genetic improvement
Organic farming
Pepper
Pimiento
Agricultura sostenible
Mejora genética
GENETICA
Máster Universitario en Mejora Genética Vegetal-Màster Universitari en Millora Genètica Vegetal
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spelling Respuesta del sistema radicular frente a la deficiencia de fósforo en el contexto de plantas injertadas, análisis fenotípico y de expresión génicaGil Villar, DanielCapsicum annuumGenetic improvementOrganic farmingPepperPimientoAgricultura sostenibleMejora genéticaGENETICAMáster Universitario en Mejora Genética Vegetal-Màster Universitari en Millora Genètica VegetalDisproportionate use of fertilizers has environmental issues derivate from soil and water bodies accumulation, as well as economic repercussions because of the increased price of agricultural inputs due to the limited availability of raw materials, is the case of phosphate rock. One of the most important horticultural product, in worldwide economic terms, is pepper (Capsicum annum L.). In Spain is one of the fifth most important crop and one which more cultivated area is dedicated, in fact, to cultivate more efficient varieties to acquisition and use of phosphorus will be positive for production economy of this vegetable. There exist previous studies where diversity of Capsicum is described. Acquisition efficiency of phosphorous is given, among other, by adaptive root morphology or by biochemical process that favour P acquisition. This is why using described tolerant varieties as rootstock could be the best solution to reduce P inputs. In addition, genomics and bioinformatic tools can be used to decode starvation response. The objectives of this work were to compare resistant pepper rootstocks for low P analyzing factors involved in root morphology and compare the genetic expression of described genes involved in P starvation tolerance. The plants were cultivated in an hydroponic system with two different P levels (0.5 mM y 1.5 mM) with Adige pepper plants grafted onto 1) `Numex X¿, 2) Piquillo, y 3) auto-grafted and controls not grafted. P levels were measured at roots and leafs, fresh weight and dry weight were recorded too. Root systems were analyzed with Winrhizo and samples of RNA from the roots were taken to analyze the expression of previously described P responsive genes. Appropriate primers were designed for genes involved in P starvation signaling, sensing, changes in metabolism and P mobilization. Plants cultivated under low P conditions showed lower levels of P concentration, however P levels in the roots were variable depending of the different rootstock used. Low P conditions wasn't severe as to make big effects over biomass. Regarding the root systems, there were differences between patterns and their response to P deficiency. Valid primers for orthologous candidate genes were designed and checked but there was no time to perform de RT-PCR reactions to know the expression level of these genes.Universitat Politècnica de ValènciaFita Fernández, Ana MaríaDepartamento de BiotecnologíaInstituto Universitario de Conservación y Mejora de la Agrodiversidad ValencianaEscuela Técnica Superior de Ingeniería Agronómica y del Medio NaturalRepositorio Institucional de la Universitat Politècnica de València Riunet20172017-12-1320172017-09-26master thesishttp://purl.org/coar/resource_type/c_bdccinfo:eu-repo/semantics/masterThesisapplication/pdfhttps://riunet.upv.es/handle/10251/92602reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valénciainstname:Universitat Politècnica de València (UPV)Españolspaopen accesshttp://purl.org/coar/access_right/c_abf2Reconocimiento - No comercial - Compartir igual (by-nc-sa) http://creativecommons.org/licenses/by-nc-sa/4.0/info:eu-repo/semantics/openAccessoai:riunet.upv.es:10251/926022026-06-13T07:49:27Z
dc.title.none.fl_str_mv Respuesta del sistema radicular frente a la deficiencia de fósforo en el contexto de plantas injertadas, análisis fenotípico y de expresión génica
title Respuesta del sistema radicular frente a la deficiencia de fósforo en el contexto de plantas injertadas, análisis fenotípico y de expresión génica
spellingShingle Respuesta del sistema radicular frente a la deficiencia de fósforo en el contexto de plantas injertadas, análisis fenotípico y de expresión génica
Gil Villar, Daniel
Capsicum annuum
Genetic improvement
Organic farming
Pepper
Pimiento
Agricultura sostenible
Mejora genética
GENETICA
Máster Universitario en Mejora Genética Vegetal-Màster Universitari en Millora Genètica Vegetal
title_short Respuesta del sistema radicular frente a la deficiencia de fósforo en el contexto de plantas injertadas, análisis fenotípico y de expresión génica
title_full Respuesta del sistema radicular frente a la deficiencia de fósforo en el contexto de plantas injertadas, análisis fenotípico y de expresión génica
title_fullStr Respuesta del sistema radicular frente a la deficiencia de fósforo en el contexto de plantas injertadas, análisis fenotípico y de expresión génica
title_full_unstemmed Respuesta del sistema radicular frente a la deficiencia de fósforo en el contexto de plantas injertadas, análisis fenotípico y de expresión génica
title_sort Respuesta del sistema radicular frente a la deficiencia de fósforo en el contexto de plantas injertadas, análisis fenotípico y de expresión génica
dc.creator.none.fl_str_mv Gil Villar, Daniel
author Gil Villar, Daniel
author_facet Gil Villar, Daniel
author_role author
dc.contributor.none.fl_str_mv Fita Fernández, Ana María
Departamento de Biotecnología
Instituto Universitario de Conservación y Mejora de la Agrodiversidad Valenciana
Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural
Repositorio Institucional de la Universitat Politècnica de València Riunet
dc.subject.none.fl_str_mv Capsicum annuum
Genetic improvement
Organic farming
Pepper
Pimiento
Agricultura sostenible
Mejora genética
GENETICA
Máster Universitario en Mejora Genética Vegetal-Màster Universitari en Millora Genètica Vegetal
topic Capsicum annuum
Genetic improvement
Organic farming
Pepper
Pimiento
Agricultura sostenible
Mejora genética
GENETICA
Máster Universitario en Mejora Genética Vegetal-Màster Universitari en Millora Genètica Vegetal
description Disproportionate use of fertilizers has environmental issues derivate from soil and water bodies accumulation, as well as economic repercussions because of the increased price of agricultural inputs due to the limited availability of raw materials, is the case of phosphate rock. One of the most important horticultural product, in worldwide economic terms, is pepper (Capsicum annum L.). In Spain is one of the fifth most important crop and one which more cultivated area is dedicated, in fact, to cultivate more efficient varieties to acquisition and use of phosphorus will be positive for production economy of this vegetable. There exist previous studies where diversity of Capsicum is described. Acquisition efficiency of phosphorous is given, among other, by adaptive root morphology or by biochemical process that favour P acquisition. This is why using described tolerant varieties as rootstock could be the best solution to reduce P inputs. In addition, genomics and bioinformatic tools can be used to decode starvation response. The objectives of this work were to compare resistant pepper rootstocks for low P analyzing factors involved in root morphology and compare the genetic expression of described genes involved in P starvation tolerance. The plants were cultivated in an hydroponic system with two different P levels (0.5 mM y 1.5 mM) with Adige pepper plants grafted onto 1) `Numex X¿, 2) Piquillo, y 3) auto-grafted and controls not grafted. P levels were measured at roots and leafs, fresh weight and dry weight were recorded too. Root systems were analyzed with Winrhizo and samples of RNA from the roots were taken to analyze the expression of previously described P responsive genes. Appropriate primers were designed for genes involved in P starvation signaling, sensing, changes in metabolism and P mobilization. Plants cultivated under low P conditions showed lower levels of P concentration, however P levels in the roots were variable depending of the different rootstock used. Low P conditions wasn't severe as to make big effects over biomass. Regarding the root systems, there were differences between patterns and their response to P deficiency. Valid primers for orthologous candidate genes were designed and checked but there was no time to perform de RT-PCR reactions to know the expression level of these genes.
publishDate 2017
dc.date.none.fl_str_mv 2017
2017-12-13
2017
2017-09-26
dc.type.none.fl_str_mv master thesis
http://purl.org/coar/resource_type/c_bdcc
dc.type.openaire.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
dc.identifier.none.fl_str_mv https://riunet.upv.es/handle/10251/92602
url https://riunet.upv.es/handle/10251/92602
dc.language.none.fl_str_mv Español
spa
language_invalid_str_mv Español
language spa
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reconocimiento - No comercial - Compartir igual (by-nc-sa)
http://creativecommons.org/licenses/by-nc-sa/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reconocimiento - No comercial - Compartir igual (by-nc-sa)
http://creativecommons.org/licenses/by-nc-sa/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universitat Politècnica de València
publisher.none.fl_str_mv Universitat Politècnica de València
dc.source.none.fl_str_mv reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
instname:Universitat Politècnica de València (UPV)
instname_str Universitat Politècnica de València (UPV)
reponame_str RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
collection RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,301603

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