Respuesta del sistema radicular frente a la deficiencia de fósforo en el contexto de plantas injertadas, análisis fenotípico y de expresión génica
Disproportionate use of fertilizers has environmental issues derivate from soil and water bodies accumulation, as well as economic repercussions because of the increased price of agricultural inputs due to the limited availability of raw materials, is the case of phosphate rock. One of the most impo...
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| Tipo de recurso: | tesis de maestría |
| Fecha de publicación: | 2017 |
| País: | España |
| Institución: | Universitat Politècnica de València (UPV) |
| Repositorio: | RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia |
| Idioma: | español |
| OAI Identifier: | oai:riunet.upv.es:10251/92602 |
| Acceso en línea: | https://riunet.upv.es/handle/10251/92602 |
| Access Level: | acceso abierto |
| Palabra clave: | Capsicum annuum Genetic improvement Organic farming Pepper Pimiento Agricultura sostenible Mejora genética GENETICA Máster Universitario en Mejora Genética Vegetal-Màster Universitari en Millora Genètica Vegetal |
| Sumario: | Disproportionate use of fertilizers has environmental issues derivate from soil and water bodies accumulation, as well as economic repercussions because of the increased price of agricultural inputs due to the limited availability of raw materials, is the case of phosphate rock. One of the most important horticultural product, in worldwide economic terms, is pepper (Capsicum annum L.). In Spain is one of the fifth most important crop and one which more cultivated area is dedicated, in fact, to cultivate more efficient varieties to acquisition and use of phosphorus will be positive for production economy of this vegetable. There exist previous studies where diversity of Capsicum is described. Acquisition efficiency of phosphorous is given, among other, by adaptive root morphology or by biochemical process that favour P acquisition. This is why using described tolerant varieties as rootstock could be the best solution to reduce P inputs. In addition, genomics and bioinformatic tools can be used to decode starvation response. The objectives of this work were to compare resistant pepper rootstocks for low P analyzing factors involved in root morphology and compare the genetic expression of described genes involved in P starvation tolerance. The plants were cultivated in an hydroponic system with two different P levels (0.5 mM y 1.5 mM) with Adige pepper plants grafted onto 1) `Numex X¿, 2) Piquillo, y 3) auto-grafted and controls not grafted. P levels were measured at roots and leafs, fresh weight and dry weight were recorded too. Root systems were analyzed with Winrhizo and samples of RNA from the roots were taken to analyze the expression of previously described P responsive genes. Appropriate primers were designed for genes involved in P starvation signaling, sensing, changes in metabolism and P mobilization. Plants cultivated under low P conditions showed lower levels of P concentration, however P levels in the roots were variable depending of the different rootstock used. Low P conditions wasn't severe as to make big effects over biomass. Regarding the root systems, there were differences between patterns and their response to P deficiency. Valid primers for orthologous candidate genes were designed and checked but there was no time to perform de RT-PCR reactions to know the expression level of these genes. |
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