Transcriptional response to metal starvation in the emerging pathogen Mycoplasma genitalium is mediated by Fur-dependent and –independent regulatory pathways

Regulador d'absorció fèrric; Mycoplasma genitalium; Patogen emergent d'ITS

Detalhes bibliográficos
Autores: Martínez-Torró, Carlos, Torres-Puig, Sergi, Monge Azemar, Marta, Sánchez-Alba, Lucía, González-Martín, Miguel, Marcos-Silva, Marina, Canals Suris, Francesc
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2019
País:España
Recursos:Departament de Salut de la Generalitat de Catalunya (DS)
Repositorio:Scientia. Dipòsit d'Informació Digital del Departament de Salut
OAI Identifier:oai:scientiasalut.gencat.cat:11351/6284
Acesso em linha:https://hdl.handle.net/11351/6284
Access Level:acceso abierto
Palavra-chave:Regulació genètica
Malalties bacterianes gramnegatives
ORGANISMS::Bacteria::Gram-Negative Bacteria::Tenericutes::Mycoplasmatales::Mycoplasmataceae::Mycoplasma::Mycoplasma genitalium
PHENOMENA AND PROCESSES::Genetic Phenomena::Gene Expression Regulation::Gene Expression Regulation, Bacterial
ORGANISMOS::Bacteria::bacterias gramnegativas::Tenericutes::Mycoplasmatales::Mycoplasmataceae::Mycoplasma::Mycoplasma genitalium
FENÓMENOS Y PROCESOS::fenómenos genéticos::regulación de la expresión génica::regulación de la expresión génica bacteriana
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spelling Transcriptional response to metal starvation in the emerging pathogen Mycoplasma genitalium is mediated by Fur-dependent and –independent regulatory pathwaysMartínez-Torró, CarlosTorres-Puig, SergiMonge Azemar, MartaSánchez-Alba, LucíaGonzález-Martín, MiguelMarcos-Silva, MarinaCanals Suris, FrancescRegulació genèticaMalalties bacterianes gramnegativesORGANISMS::Bacteria::Gram-Negative Bacteria::Tenericutes::Mycoplasmatales::Mycoplasmataceae::Mycoplasma::Mycoplasma genitaliumPHENOMENA AND PROCESSES::Genetic Phenomena::Gene Expression Regulation::Gene Expression Regulation, BacterialORGANISMOS::Bacteria::bacterias gramnegativas::Tenericutes::Mycoplasmatales::Mycoplasmataceae::Mycoplasma::Mycoplasma genitaliumFENÓMENOS Y PROCESOS::fenómenos genéticos::regulación de la expresión génica::regulación de la expresión génica bacterianaRegulador d'absorció fèrric; Mycoplasma genitalium; Patogen emergent d'ITSRegulador de la absorción férrico; Mycoplasma genitalium; Patógeno emergente de ITSFerric uptake regulator; Mycoplasma genitalium; Emerging STI pathogenTransition metals participate in numerous enzymatic reactions and they are essential for survival in all living organisms. For this reason, bacterial pathogens have evolved dedicated machineries to effectively compete with their hosts and scavenge metals at the site of infection. In this study, we investigated the mechanisms controlling metal acquisition in the emerging human pathogen Mycoplasma genitalium. We observed a robust transcriptional response to metal starvation, and many genes coding for predicted lipoproteins and ABC-transporters were significantly up-regulated. Transcriptional analysis of a mutant strain lacking a metalloregulator of the Fur family revealed the activation of a full operon encoding a putative metal transporter system and a gene coding for a Histidine-rich lipoprotein (Hrl). We recognized a conserved sequence with dyad symmetry within the promoter region of the Fur-regulated genes. Mutagenesis of the predicted Fur operator within the hrl promoter abrogated Fur- and metal-dependent expression of a reporter gene. Metal starvation still impelled a strong transcriptional response in the fur mutant, demonstrating the existence of Fur-independent regulatory pathways controlling metal homeostasis. Finally, analysis of metal accumulation in the wild-type strain and the fur mutant by ICP-MS revealed an important role of Fur in nickel acquisition.This work was supported by the Ministerio de Ciencia, Innovación y Universidades (Grant BIO2017-84166-R). The Proteomics Unit at VHIO is a member ProteoRed, PRB3 (Grant IPT17/0019 – ISCIII-SGEFI/ERDF).Taylor and FrancisInstitut Català de la Salut[Martínez-Torró C, Torres-Puig S, Sánchez-Alba L, González-Martín M, Marcos-Silva M] Institut de Biotecnologia i Biomedicina and Departament de Bioquímica i Biologia Molecular, Universitat Autònoma de Barcelona, Bellaterra, Spain. [Monge M, Canals F] Vall d’Hebron Institute of Oncology (VHIO), Barcelona, SpainVall d'Hebron Barcelona Hospital Campus2021202120192020info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfimage/jpegapplication/pdfapplication/pdfhttps://hdl.handle.net/11351/6284Scientiareponame:Scientia. Dipòsit d'Informació Digital del Departament de Salutinstname:Departament de Salut de la Generalitat de Catalunya (DS)InglésEmerging Microbes & Infections;9https://doi.org/10.1080/22221751.2019.1700762info:eu-repo/grantAgreement/ES/PE2013-2016/BIO2017-84166-RAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:scientiasalut.gencat.cat:11351/62842026-06-12T09:38:37Z
dc.title.none.fl_str_mv Transcriptional response to metal starvation in the emerging pathogen Mycoplasma genitalium is mediated by Fur-dependent and –independent regulatory pathways
title Transcriptional response to metal starvation in the emerging pathogen Mycoplasma genitalium is mediated by Fur-dependent and –independent regulatory pathways
spellingShingle Transcriptional response to metal starvation in the emerging pathogen Mycoplasma genitalium is mediated by Fur-dependent and –independent regulatory pathways
Martínez-Torró, Carlos
Regulació genètica
Malalties bacterianes gramnegatives
ORGANISMS::Bacteria::Gram-Negative Bacteria::Tenericutes::Mycoplasmatales::Mycoplasmataceae::Mycoplasma::Mycoplasma genitalium
PHENOMENA AND PROCESSES::Genetic Phenomena::Gene Expression Regulation::Gene Expression Regulation, Bacterial
ORGANISMOS::Bacteria::bacterias gramnegativas::Tenericutes::Mycoplasmatales::Mycoplasmataceae::Mycoplasma::Mycoplasma genitalium
FENÓMENOS Y PROCESOS::fenómenos genéticos::regulación de la expresión génica::regulación de la expresión génica bacteriana
title_short Transcriptional response to metal starvation in the emerging pathogen Mycoplasma genitalium is mediated by Fur-dependent and –independent regulatory pathways
title_full Transcriptional response to metal starvation in the emerging pathogen Mycoplasma genitalium is mediated by Fur-dependent and –independent regulatory pathways
title_fullStr Transcriptional response to metal starvation in the emerging pathogen Mycoplasma genitalium is mediated by Fur-dependent and –independent regulatory pathways
title_full_unstemmed Transcriptional response to metal starvation in the emerging pathogen Mycoplasma genitalium is mediated by Fur-dependent and –independent regulatory pathways
title_sort Transcriptional response to metal starvation in the emerging pathogen Mycoplasma genitalium is mediated by Fur-dependent and –independent regulatory pathways
dc.creator.none.fl_str_mv Martínez-Torró, Carlos
Torres-Puig, Sergi
Monge Azemar, Marta
Sánchez-Alba, Lucía
González-Martín, Miguel
Marcos-Silva, Marina
Canals Suris, Francesc
author Martínez-Torró, Carlos
author_facet Martínez-Torró, Carlos
Torres-Puig, Sergi
Monge Azemar, Marta
Sánchez-Alba, Lucía
González-Martín, Miguel
Marcos-Silva, Marina
Canals Suris, Francesc
author_role author
author2 Torres-Puig, Sergi
Monge Azemar, Marta
Sánchez-Alba, Lucía
González-Martín, Miguel
Marcos-Silva, Marina
Canals Suris, Francesc
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Institut Català de la Salut
[Martínez-Torró C, Torres-Puig S, Sánchez-Alba L, González-Martín M, Marcos-Silva M] Institut de Biotecnologia i Biomedicina and Departament de Bioquímica i Biologia Molecular, Universitat Autònoma de Barcelona, Bellaterra, Spain. [Monge M, Canals F] Vall d’Hebron Institute of Oncology (VHIO), Barcelona, Spain
Vall d'Hebron Barcelona Hospital Campus
dc.subject.none.fl_str_mv Regulació genètica
Malalties bacterianes gramnegatives
ORGANISMS::Bacteria::Gram-Negative Bacteria::Tenericutes::Mycoplasmatales::Mycoplasmataceae::Mycoplasma::Mycoplasma genitalium
PHENOMENA AND PROCESSES::Genetic Phenomena::Gene Expression Regulation::Gene Expression Regulation, Bacterial
ORGANISMOS::Bacteria::bacterias gramnegativas::Tenericutes::Mycoplasmatales::Mycoplasmataceae::Mycoplasma::Mycoplasma genitalium
FENÓMENOS Y PROCESOS::fenómenos genéticos::regulación de la expresión génica::regulación de la expresión génica bacteriana
topic Regulació genètica
Malalties bacterianes gramnegatives
ORGANISMS::Bacteria::Gram-Negative Bacteria::Tenericutes::Mycoplasmatales::Mycoplasmataceae::Mycoplasma::Mycoplasma genitalium
PHENOMENA AND PROCESSES::Genetic Phenomena::Gene Expression Regulation::Gene Expression Regulation, Bacterial
ORGANISMOS::Bacteria::bacterias gramnegativas::Tenericutes::Mycoplasmatales::Mycoplasmataceae::Mycoplasma::Mycoplasma genitalium
FENÓMENOS Y PROCESOS::fenómenos genéticos::regulación de la expresión génica::regulación de la expresión génica bacteriana
description Regulador d'absorció fèrric; Mycoplasma genitalium; Patogen emergent d'ITS
publishDate 2019
dc.date.none.fl_str_mv 2019
2020
2021
2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/11351/6284
url https://hdl.handle.net/11351/6284
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Emerging Microbes & Infections;9
https://doi.org/10.1080/22221751.2019.1700762
info:eu-repo/grantAgreement/ES/PE2013-2016/BIO2017-84166-R
dc.rights.none.fl_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
image/jpeg
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Taylor and Francis
publisher.none.fl_str_mv Taylor and Francis
dc.source.none.fl_str_mv Scientia
reponame:Scientia. Dipòsit d'Informació Digital del Departament de Salut
instname:Departament de Salut de la Generalitat de Catalunya (DS)
instname_str Departament de Salut de la Generalitat de Catalunya (DS)
reponame_str Scientia. Dipòsit d'Informació Digital del Departament de Salut
collection Scientia. Dipòsit d'Informació Digital del Departament de Salut
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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