Max1 links MBF dependent transcription upon completion of DNA synthesis in fission yeast
When DNA replication is challenged, cells activate a DNA synthesis checkpoint blocking cell cycle progression until they are able to overcome the replication defects. In fission yeast, Cds1 is the effector kinase of this checkpoint, inhibiting M phase entry, stabilizing stalled replication forks and...
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| Tipo de recurso: | tesis doctoral |
| Estado: | Versión publicada |
| Fecha de publicación: | 2010 |
| País: | España |
| Institución: | CBUC, CESCA |
| Repositorio: | TDR. Tesis Doctorales en Red |
| OAI Identifier: | oai:www.tdx.cat:10803/7223 |
| Acceso en línea: | http://www.tdx.cat/TDX-0207111-150509 http://hdl.handle.net/10803/7223 |
| Access Level: | acceso abierto |
| Palabra clave: | stress kinase phosphorylation DNA damage Checkpoint Cell Cycle transcription factor transcription replication yeast estrés kinasa fosforilación daño en DNA checkpoint ciclo celular factor de transcripción transcripción replicación Schizosaccharomyces pombe levadura 577 |
| Sumario: | When DNA replication is challenged, cells activate a DNA synthesis checkpoint blocking cell cycle progression until they are able to overcome the replication defects. In fission yeast, Cds1 is the effector kinase of this checkpoint, inhibiting M phase entry, stabilizing stalled replication forks and triggering transcriptional activation of S-phase genes; the molecular basis of this last effect remains largely unknown. The MBF complex controls the transcription of S-phase genes. We have purified novel interactors of the MBF complex and among them we have identified the repressor Max1. When the DNA synthesis checkpoint is activated, Max1 is phosphorylated by Cds1 resulting in the abrogation of its binding to MBF. As a consequence, MBF-dependent transcription is maintained active until cells are able to overcome this challenge. |
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