Different Toxicity Mechanisms for Citrinin and Ochratoxin A Revealed by Transcriptomic Analysis in Yeast

[EN] Citrinin (CIT) and ochratoxin A (OTA) are important mycotoxins, which frequently co-contaminate foodstuff. In order to assess the toxicologic threat posed by the two mycotoxins separately or in combination, their biological effects were studied here using genomic transcription profiling and spe...

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Autores: Vanacloig-Pedrós, Elena, Proft, Markus Hans, Pascual-Ahuir Giner, María Desamparados|||0000-0001-6173-1526
Tipo de recurso: artículo
Fecha de publicación:2016
País:España
Institución:Universitat Politècnica de València (UPV)
Repositorio:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
Idioma:inglés
OAI Identifier:oai:riunet.upv.es:10251/150635
Acceso en línea:https://riunet.upv.es/handle/10251/150635
Access Level:acceso abierto
Palabra clave:Ochratoxin A
Citrinin
Transcriptome
Saccharomyces cerevisiae
Mycotoxins
Oxidative stress
Dose response
BIOQUIMICA Y BIOLOGIA MOLECULAR
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spelling Different Toxicity Mechanisms for Citrinin and Ochratoxin A Revealed by Transcriptomic Analysis in YeastVanacloig-Pedrós, ElenaProft, Markus HansPascual-Ahuir Giner, María Desamparados|||0000-0001-6173-1526Ochratoxin ACitrininTranscriptomeSaccharomyces cerevisiaeMycotoxinsOxidative stressDose responseBIOQUIMICA Y BIOLOGIA MOLECULAR[EN] Citrinin (CIT) and ochratoxin A (OTA) are important mycotoxins, which frequently co-contaminate foodstuff. In order to assess the toxicologic threat posed by the two mycotoxins separately or in combination, their biological effects were studied here using genomic transcription profiling and specific live cell gene expression reporters in yeast cells. Both CIT and OTA cause highly transient transcriptional activation of different stress genes, which is greatly enhanced by the disruption of the multidrug exporter Pdr5. Therefore, we performed genome-wide transcription profiling experiments with the pdr5 mutant in response to acute CIT, OTA, or combined CIT/OTA exposure. We found that CIT and OTA activate divergent and largely nonoverlapping gene sets in yeast. CIT mainly caused the rapid induction of antioxidant and drug extrusion-related gene functions, while OTA mainly deregulated developmental genes related with yeast sporulation and sexual reproduction, having only a minor effect on the antioxidant response. The simultaneous exposure to CIT and OTA gave rise to a genomic response, which combined the specific features of the separated mycotoxin treatments. The application of stress-specific mutants and reporter gene fusions further confirmed that both mycotoxins have divergent biological effects in cells. Our results indicate that CIT exposure causes a strong oxidative stress, which triggers a massive transcriptional antioxidant and drug extrusion response, while OTA mainly deregulates developmental genes and only marginally induces the antioxidant defense.We thank Lorena Latorre and Javier Forment for their help with the microarray experiments and data analysis. This work was funded only in the initial phase by a grant from Ministerio de Economía y Competitividad (BFU2011-23326). We thank the Fond for Open Access Publication from Consejo Superior de Investigaciones Científicas (CSIC) for supporting publication costs of this article.MDPI AGDepartamento de BiotecnologíaEscuela Técnica Superior de Ingeniería Agronómica y del Medio NaturalCentro de Investigación e Innovación en BioingenieríaMinisterio de Ciencia, Innovación y UniversidadesConsejo Superior de Investigaciones CientíficasRepositorio Institucional de la Universitat Politècnica de València Riunet20162016-09-22journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://riunet.upv.es/handle/10251/150635reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valénciainstname:Universitat Politècnica de València (UPV)InglésengMinisterio de Ciencia e Innovación http://dx.doi.org/10.13039/501100004837 BFU2011-23326 REGULACION DE LA CROMATINA Y DE LA ESTRUCTURA MITOCONDRIAL EN RESPUESTA A ESTRES OSMOTICOopen accesshttp://purl.org/coar/access_right/c_abf2Reconocimiento (by)http://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:riunet.upv.es:10251/1506352026-06-13T07:49:27Z
dc.title.none.fl_str_mv Different Toxicity Mechanisms for Citrinin and Ochratoxin A Revealed by Transcriptomic Analysis in Yeast
title Different Toxicity Mechanisms for Citrinin and Ochratoxin A Revealed by Transcriptomic Analysis in Yeast
spellingShingle Different Toxicity Mechanisms for Citrinin and Ochratoxin A Revealed by Transcriptomic Analysis in Yeast
Vanacloig-Pedrós, Elena
Ochratoxin A
Citrinin
Transcriptome
Saccharomyces cerevisiae
Mycotoxins
Oxidative stress
Dose response
BIOQUIMICA Y BIOLOGIA MOLECULAR
title_short Different Toxicity Mechanisms for Citrinin and Ochratoxin A Revealed by Transcriptomic Analysis in Yeast
title_full Different Toxicity Mechanisms for Citrinin and Ochratoxin A Revealed by Transcriptomic Analysis in Yeast
title_fullStr Different Toxicity Mechanisms for Citrinin and Ochratoxin A Revealed by Transcriptomic Analysis in Yeast
title_full_unstemmed Different Toxicity Mechanisms for Citrinin and Ochratoxin A Revealed by Transcriptomic Analysis in Yeast
title_sort Different Toxicity Mechanisms for Citrinin and Ochratoxin A Revealed by Transcriptomic Analysis in Yeast
dc.creator.none.fl_str_mv Vanacloig-Pedrós, Elena
Proft, Markus Hans
Pascual-Ahuir Giner, María Desamparados|||0000-0001-6173-1526
author Vanacloig-Pedrós, Elena
author_facet Vanacloig-Pedrós, Elena
Proft, Markus Hans
Pascual-Ahuir Giner, María Desamparados|||0000-0001-6173-1526
author_role author
author2 Proft, Markus Hans
Pascual-Ahuir Giner, María Desamparados|||0000-0001-6173-1526
author2_role author
author
dc.contributor.none.fl_str_mv Departamento de Biotecnología
Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural
Centro de Investigación e Innovación en Bioingeniería
Ministerio de Ciencia, Innovación y Universidades
Consejo Superior de Investigaciones Científicas
Repositorio Institucional de la Universitat Politècnica de València Riunet
dc.subject.none.fl_str_mv Ochratoxin A
Citrinin
Transcriptome
Saccharomyces cerevisiae
Mycotoxins
Oxidative stress
Dose response
BIOQUIMICA Y BIOLOGIA MOLECULAR
topic Ochratoxin A
Citrinin
Transcriptome
Saccharomyces cerevisiae
Mycotoxins
Oxidative stress
Dose response
BIOQUIMICA Y BIOLOGIA MOLECULAR
description [EN] Citrinin (CIT) and ochratoxin A (OTA) are important mycotoxins, which frequently co-contaminate foodstuff. In order to assess the toxicologic threat posed by the two mycotoxins separately or in combination, their biological effects were studied here using genomic transcription profiling and specific live cell gene expression reporters in yeast cells. Both CIT and OTA cause highly transient transcriptional activation of different stress genes, which is greatly enhanced by the disruption of the multidrug exporter Pdr5. Therefore, we performed genome-wide transcription profiling experiments with the pdr5 mutant in response to acute CIT, OTA, or combined CIT/OTA exposure. We found that CIT and OTA activate divergent and largely nonoverlapping gene sets in yeast. CIT mainly caused the rapid induction of antioxidant and drug extrusion-related gene functions, while OTA mainly deregulated developmental genes related with yeast sporulation and sexual reproduction, having only a minor effect on the antioxidant response. The simultaneous exposure to CIT and OTA gave rise to a genomic response, which combined the specific features of the separated mycotoxin treatments. The application of stress-specific mutants and reporter gene fusions further confirmed that both mycotoxins have divergent biological effects in cells. Our results indicate that CIT exposure causes a strong oxidative stress, which triggers a massive transcriptional antioxidant and drug extrusion response, while OTA mainly deregulates developmental genes and only marginally induces the antioxidant defense.
publishDate 2016
dc.date.none.fl_str_mv 2016
2016-09-22
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://riunet.upv.es/handle/10251/150635
url https://riunet.upv.es/handle/10251/150635
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Ministerio de Ciencia e Innovación http://dx.doi.org/10.13039/501100004837 BFU2011-23326 REGULACION DE LA CROMATINA Y DE LA ESTRUCTURA MITOCONDRIAL EN RESPUESTA A ESTRES OSMOTICO
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reconocimiento (by)
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reconocimiento (by)
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI AG
publisher.none.fl_str_mv MDPI AG
dc.source.none.fl_str_mv reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
instname:Universitat Politècnica de València (UPV)
instname_str Universitat Politècnica de València (UPV)
reponame_str RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
collection RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,301603

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