Differential contribution of T-type voltage-gated calcium channels to vascular reactivity in the aorta and renal artery of healthy rabbits

T-type voltage-gated calcium channels (VGCCs) are comparatively understudied in large conduit artery function relative to the well-characterized L-type channel subtype. This study investigates the vascular roles of T-type VGCCs in the aorta and renal artery of healthy rabbits using functional reacti...

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Autores: Suarez, A, Guerra-Ojeda, S, Zarzoso, M, Serna-García, M, Vila, JM, Serna, E, Mauricio, MD
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2025
País:España
Institución:INCLIVA
Repositorio:r-INCLIVA. Repositorio Institucional de Producción Científica de INCLIVA
OAI Identifier:oai:incliva.fundanetsuite.com:p20450
Acceso en línea:https://incliva.portalinvestigacion.com/publicaciones/20450
Access Level:acceso abierto
Palabra clave:aorta
endothelium
eNOS
renal artery
T-type voltage-gated calcium channels
vascular reactivity
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spelling Differential contribution of T-type voltage-gated calcium channels to vascular reactivity in the aorta and renal artery of healthy rabbitsSuarez, AGuerra-Ojeda, SZarzoso, MSerna-García, MVila, JMSerna, EMauricio, MDaortaendotheliumeNOSrenal arteryT-type voltage-gated calcium channelsvascular reactivityT-type voltage-gated calcium channels (VGCCs) are comparatively understudied in large conduit artery function relative to the well-characterized L-type channel subtype. This study investigates the vascular roles of T-type VGCCs in the aorta and renal artery of healthy rabbits using functional reactivity assays and immunofluorescence and further elucidates the interaction between T-type VGCC activity and the nitric oxide (NO) signalling pathway. T-type VGCCs contributed to contractile responses induced by phenylephrine and angiotensin II in both arteries. Moreover, in the renal artery, the contribution of T-type VGCCs in response to phenylephrine increased in the absence of NO and was associated with endothelium-dependent vasodilation. Immunofluorescence analysis revealed co-localization of CaV3.1 with endothelial nitric oxide synthase in the renal artery, but not in the aorta, suggesting a vasodilatory role in renal circulation. These findings highlight vascular bed-specific functions of T-type VGCCs and their interaction with endothelial pathways in the renal artery.WILEY2025info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://incliva.portalinvestigacion.com/publicaciones/20450EXPERIMENTAL PHYSIOLOGYISSN: 09580670ISSNe: 1469445Xreponame:r-INCLIVA. Repositorio Institucional de Producción Científica de INCLIVAinstname:INCLIVAInglésinfo:eu-repo/semantics/openAccessoai:incliva.fundanetsuite.com:p204502026-06-07T16:35:31Z
dc.title.none.fl_str_mv Differential contribution of T-type voltage-gated calcium channels to vascular reactivity in the aorta and renal artery of healthy rabbits
title Differential contribution of T-type voltage-gated calcium channels to vascular reactivity in the aorta and renal artery of healthy rabbits
spellingShingle Differential contribution of T-type voltage-gated calcium channels to vascular reactivity in the aorta and renal artery of healthy rabbits
Suarez, A
aorta
endothelium
eNOS
renal artery
T-type voltage-gated calcium channels
vascular reactivity
title_short Differential contribution of T-type voltage-gated calcium channels to vascular reactivity in the aorta and renal artery of healthy rabbits
title_full Differential contribution of T-type voltage-gated calcium channels to vascular reactivity in the aorta and renal artery of healthy rabbits
title_fullStr Differential contribution of T-type voltage-gated calcium channels to vascular reactivity in the aorta and renal artery of healthy rabbits
title_full_unstemmed Differential contribution of T-type voltage-gated calcium channels to vascular reactivity in the aorta and renal artery of healthy rabbits
title_sort Differential contribution of T-type voltage-gated calcium channels to vascular reactivity in the aorta and renal artery of healthy rabbits
dc.creator.none.fl_str_mv Suarez, A
Guerra-Ojeda, S
Zarzoso, M
Serna-García, M
Vila, JM
Serna, E
Mauricio, MD
author Suarez, A
author_facet Suarez, A
Guerra-Ojeda, S
Zarzoso, M
Serna-García, M
Vila, JM
Serna, E
Mauricio, MD
author_role author
author2 Guerra-Ojeda, S
Zarzoso, M
Serna-García, M
Vila, JM
Serna, E
Mauricio, MD
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv aorta
endothelium
eNOS
renal artery
T-type voltage-gated calcium channels
vascular reactivity
topic aorta
endothelium
eNOS
renal artery
T-type voltage-gated calcium channels
vascular reactivity
description T-type voltage-gated calcium channels (VGCCs) are comparatively understudied in large conduit artery function relative to the well-characterized L-type channel subtype. This study investigates the vascular roles of T-type VGCCs in the aorta and renal artery of healthy rabbits using functional reactivity assays and immunofluorescence and further elucidates the interaction between T-type VGCC activity and the nitric oxide (NO) signalling pathway. T-type VGCCs contributed to contractile responses induced by phenylephrine and angiotensin II in both arteries. Moreover, in the renal artery, the contribution of T-type VGCCs in response to phenylephrine increased in the absence of NO and was associated with endothelium-dependent vasodilation. Immunofluorescence analysis revealed co-localization of CaV3.1 with endothelial nitric oxide synthase in the renal artery, but not in the aorta, suggesting a vasodilatory role in renal circulation. These findings highlight vascular bed-specific functions of T-type VGCCs and their interaction with endothelial pathways in the renal artery.
publishDate 2025
dc.date.none.fl_str_mv 2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://incliva.portalinvestigacion.com/publicaciones/20450
url https://incliva.portalinvestigacion.com/publicaciones/20450
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv WILEY
publisher.none.fl_str_mv WILEY
dc.source.none.fl_str_mv EXPERIMENTAL PHYSIOLOGY
ISSN: 09580670
ISSNe: 1469445X
reponame:r-INCLIVA. Repositorio Institucional de Producción Científica de INCLIVA
instname:INCLIVA
instname_str INCLIVA
reponame_str r-INCLIVA. Repositorio Institucional de Producción Científica de INCLIVA
collection r-INCLIVA. Repositorio Institucional de Producción Científica de INCLIVA
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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