An iridium oxide nanoparticle and polythionine thin film based platform for sensitive Leishmania DNA detection

An impedimetric label-free genosensor for high sensitive DNA detection is developed. This system is based on a screen-printed carbon electrode modified with the thionine layer and iridium oxide nanoparticles (IrO₂ NP/). An aminated oligonucleotide probe is immobilized on the IrO₂ NP/polythionine mod...

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Detalles Bibliográficos
Autores: Mayorga Martinez, Carmen, Chamorro García, Alejandro|||0000-0003-0953-5848, Serrano, Lorena, Rivas, Lourdes|||0000-0002-1510-5927, Quesada-González, Daniel|||0000-0003-3064-2146, Altet Sanahujes, Laura, Francino, Olga|||0000-0002-9022-3835, Sánchez Bonastre, Armando|||0000-0001-9160-1124, Merkoçi, Arben|||0000-0003-2486-8085
Tipo de recurso: artículo
Fecha de publicación:2015
País:España
Institución:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:200181
Acceso en línea:https://ddd.uab.cat/record/200181
https://dx.doi.org/urn:doi:10.1039/c5tb00545k
Access Level:acceso abierto
Palabra clave:Blocking agents
DNA detection
Genosensors
Iridium oxides
Modified electrodes
Oligonucleotide probes
Reproducibilities
Screen-printed carbon electrodes
Descripción
Sumario:An impedimetric label-free genosensor for high sensitive DNA detection is developed. This system is based on a screen-printed carbon electrode modified with the thionine layer and iridium oxide nanoparticles (IrO₂ NP/). An aminated oligonucleotide probe is immobilized on the IrO₂ NP/polythionine modified electrode and ethanolamine was used as a blocking agent. Different diluted PCR amplified DNA samples have been detected. The selectivity and reproducibility of this system are studied and the system was highly reproducible with RSD ≈ 15% and sensitive enough while using 2% of ethanolamine during the blocking step employed for genosensor preparation.

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