Lipophilicity determination of acidic compounds: MEEKC as a reliable high-throughput methodology
In the present study apressure-assisted MEEKC method with reversed-polarity using a conventional CE instrument with UV detection and uncoated fused silica capillariesis validated as a high-throughput methodology for the lipophilicity determination of the neutral species of acidic compounds(pKa> 3...
| Autores: | , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2018 |
| País: | España |
| Institución: | Universidad de Barcelona |
| Repositorio: | Dipòsit Digital de la UB |
| OAI Identifier: | oai:diposit.ub.edu:2445/123114 |
| Acceso en línea: | https://hdl.handle.net/2445/123114 |
| Access Level: | acceso abierto |
| Palabra clave: | Electroforesi capil·lar Cromatografia de líquids Lipofília Capillary electrophoresis Liquid chromatography Lipophilicity |
| Sumario: | In the present study apressure-assisted MEEKC method with reversed-polarity using a conventional CE instrument with UV detection and uncoated fused silica capillariesis validated as a high-throughput methodology for the lipophilicity determination of the neutral species of acidic compounds(pKa> 3.5).After the calibration of the system with four standard compoundsof known log Po/w, mass distribution ratios (log kMEEKC) of new molecules can be directly converted into log Po/wvalues by means of a simple linear equation (log Po/w=a·log kMEEKC+b). The method was internally and externally validated for a log Po/wrange between-1.54 and4.75, with higheraccuraciesthan conventional liquid chromatographic methods. |
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