Layer-by-layer decorated nanoparticles with tunable antibacterial and antibiofilm properties against both gram-positive and gram-negative bacteria

acteria-mediated diseases are a global healthcare concern due to the development and spread of antibiotic resistant strains. Cationic compounds are considered membrane active biocidal agents having a great potential to control bacterial infections, while limiting the emergence of drug resistance. He...

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Autores: Ivanova, Aleksandra Asenova|||0000-0002-7210-8905, Ivanova, Kristina Dimitrova|||0000-0001-9158-4088, Hoyo Pérez, Javier|||0000-0002-9927-2465, Sanchez-Gomez, Susana, Heinze, Thomas, Tzanov, Tzanko|||0000-0002-8568-1110
Tipo de recurso: artículo
Fecha de publicación:2018
País:España
Institución:Universitat Politècnica de Catalunya (UPC)
Repositorio:UPCommons. Portal del coneixement obert de la UPC
Idioma:inglés
OAI Identifier:oai:upcommons.upc.edu:2117/113123
Acceso en línea:https://hdl.handle.net/2117/113123
https://dx.doi.org/10.1021/acsami.7b16508
Access Level:acceso abierto
Palabra clave:Nanoparticles--Therapeutic use
Biopolymers
Bioengineering
Aminocellulose
antibacterial nanoparticles
biofilm prevention
hyaluronic acid
layer-by-layer
Nanopartícules -- Ús terapèutic
Biopolímers
Bioenginyeria
Àrees temàtiques de la UPC::Enginyeria química
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spelling Layer-by-layer decorated nanoparticles with tunable antibacterial and antibiofilm properties against both gram-positive and gram-negative bacteriaIvanova, Aleksandra Asenova|||0000-0002-7210-8905Ivanova, Kristina Dimitrova|||0000-0001-9158-4088Hoyo Pérez, Javier|||0000-0002-9927-2465Sanchez-Gomez, SusanaHeinze, ThomasTzanov, Tzanko|||0000-0002-8568-1110Nanoparticles--Therapeutic useBiopolymersBioengineeringAminocelluloseantibacterial nanoparticlesbiofilm preventionhyaluronic acidlayer-by-layerNanopartícules -- Ús terapèuticBiopolímersBioenginyeriaÀrees temàtiques de la UPC::Enginyeria químicaacteria-mediated diseases are a global healthcare concern due to the development and spread of antibiotic resistant strains. Cationic compounds are considered membrane active biocidal agents having a great potential to control bacterial infections, while limiting the emergence of drug resistance. Herein, the versatile and simple Layer-by-Layer (LbL) technique was used to coat alternating multilayers of an antibacterial aminocellulose conjugate and the biocompatible hyaluronic acid on biocompatible polymer nanoparticles (NPs), taking advantage of the nano-size of these otherwise biologically inert templates. Stable polyelectrolyte-decorated particles with an average size of 50 nm and zeta potential of + 40.6 mV were developed after five LbL assembly cycles. The antibacterial activity of these NPs against the Gram-positive Staphylococcus aureus (S. aureus) and Gram-negative Escherichia coli (E. coli) increased significantly when the polycationic aminocellulose was in the outermost layer. The large number of amino groups available on the particles surface, together with the nano-size of the multilayer conjugates, improved their interaction with bacterial membrane phospholipids leading to membrane disruption, as confirmed by a Langmuir monolayer model, and the 10 logs reduction for both bacteria. The biopolymer decorated NPs were also able to inhibit the biofilm formation of S. aureus and E. coli by 94 and 40%, respectively, without affecting human cells viability. The use of LbL coated NPs appears as a promising antibiotic-free alternative for controlling bacterial infections using low amount of antimicrobial agent.Peer Reviewed20182018-01-0920182018-01-24journal articlehttp://purl.org/coar/resource_type/c_6501AMhttp://purl.org/coar/version/c_ab4af688f83e57aainfo:eu-repo/semantics/articleapplication/vnd.openxmlformats-officedocument.wordprocessingml.documenthttps://hdl.handle.net/2117/113123https://dx.doi.org/10.1021/acsami.7b16508reponame:UPCommons. Portal del coneixement obert de la UPCinstname:Universitat Politècnica de Catalunya (UPC)InglésengEuropean Commission http://doi.org/10.13039/100010661 Horizon 2020 Framework Programme 685909 SKin Healthcare by Innovative NanoCAPsuleSopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivs 3.0 Spainhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/info:eu-repo/semantics/openAccessoai:upcommons.upc.edu:2117/1131232026-05-27T15:37:01Z
dc.title.none.fl_str_mv Layer-by-layer decorated nanoparticles with tunable antibacterial and antibiofilm properties against both gram-positive and gram-negative bacteria
title Layer-by-layer decorated nanoparticles with tunable antibacterial and antibiofilm properties against both gram-positive and gram-negative bacteria
spellingShingle Layer-by-layer decorated nanoparticles with tunable antibacterial and antibiofilm properties against both gram-positive and gram-negative bacteria
Ivanova, Aleksandra Asenova|||0000-0002-7210-8905
Nanoparticles--Therapeutic use
Biopolymers
Bioengineering
Aminocellulose
antibacterial nanoparticles
biofilm prevention
hyaluronic acid
layer-by-layer
Nanopartícules -- Ús terapèutic
Biopolímers
Bioenginyeria
Àrees temàtiques de la UPC::Enginyeria química
title_short Layer-by-layer decorated nanoparticles with tunable antibacterial and antibiofilm properties against both gram-positive and gram-negative bacteria
title_full Layer-by-layer decorated nanoparticles with tunable antibacterial and antibiofilm properties against both gram-positive and gram-negative bacteria
title_fullStr Layer-by-layer decorated nanoparticles with tunable antibacterial and antibiofilm properties against both gram-positive and gram-negative bacteria
title_full_unstemmed Layer-by-layer decorated nanoparticles with tunable antibacterial and antibiofilm properties against both gram-positive and gram-negative bacteria
title_sort Layer-by-layer decorated nanoparticles with tunable antibacterial and antibiofilm properties against both gram-positive and gram-negative bacteria
dc.creator.none.fl_str_mv Ivanova, Aleksandra Asenova|||0000-0002-7210-8905
Ivanova, Kristina Dimitrova|||0000-0001-9158-4088
Hoyo Pérez, Javier|||0000-0002-9927-2465
Sanchez-Gomez, Susana
Heinze, Thomas
Tzanov, Tzanko|||0000-0002-8568-1110
author Ivanova, Aleksandra Asenova|||0000-0002-7210-8905
author_facet Ivanova, Aleksandra Asenova|||0000-0002-7210-8905
Ivanova, Kristina Dimitrova|||0000-0001-9158-4088
Hoyo Pérez, Javier|||0000-0002-9927-2465
Sanchez-Gomez, Susana
Heinze, Thomas
Tzanov, Tzanko|||0000-0002-8568-1110
author_role author
author2 Ivanova, Kristina Dimitrova|||0000-0001-9158-4088
Hoyo Pérez, Javier|||0000-0002-9927-2465
Sanchez-Gomez, Susana
Heinze, Thomas
Tzanov, Tzanko|||0000-0002-8568-1110
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Nanoparticles--Therapeutic use
Biopolymers
Bioengineering
Aminocellulose
antibacterial nanoparticles
biofilm prevention
hyaluronic acid
layer-by-layer
Nanopartícules -- Ús terapèutic
Biopolímers
Bioenginyeria
Àrees temàtiques de la UPC::Enginyeria química
topic Nanoparticles--Therapeutic use
Biopolymers
Bioengineering
Aminocellulose
antibacterial nanoparticles
biofilm prevention
hyaluronic acid
layer-by-layer
Nanopartícules -- Ús terapèutic
Biopolímers
Bioenginyeria
Àrees temàtiques de la UPC::Enginyeria química
description acteria-mediated diseases are a global healthcare concern due to the development and spread of antibiotic resistant strains. Cationic compounds are considered membrane active biocidal agents having a great potential to control bacterial infections, while limiting the emergence of drug resistance. Herein, the versatile and simple Layer-by-Layer (LbL) technique was used to coat alternating multilayers of an antibacterial aminocellulose conjugate and the biocompatible hyaluronic acid on biocompatible polymer nanoparticles (NPs), taking advantage of the nano-size of these otherwise biologically inert templates. Stable polyelectrolyte-decorated particles with an average size of 50 nm and zeta potential of + 40.6 mV were developed after five LbL assembly cycles. The antibacterial activity of these NPs against the Gram-positive Staphylococcus aureus (S. aureus) and Gram-negative Escherichia coli (E. coli) increased significantly when the polycationic aminocellulose was in the outermost layer. The large number of amino groups available on the particles surface, together with the nano-size of the multilayer conjugates, improved their interaction with bacterial membrane phospholipids leading to membrane disruption, as confirmed by a Langmuir monolayer model, and the 10 logs reduction for both bacteria. The biopolymer decorated NPs were also able to inhibit the biofilm formation of S. aureus and E. coli by 94 and 40%, respectively, without affecting human cells viability. The use of LbL coated NPs appears as a promising antibiotic-free alternative for controlling bacterial infections using low amount of antimicrobial agent.
publishDate 2018
dc.date.none.fl_str_mv 2018
2018-01-09
2018
2018-01-24
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
AM
http://purl.org/coar/version/c_ab4af688f83e57aa
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/2117/113123
https://dx.doi.org/10.1021/acsami.7b16508
url https://hdl.handle.net/2117/113123
https://dx.doi.org/10.1021/acsami.7b16508
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv European Commission http://doi.org/10.13039/100010661 Horizon 2020 Framework Programme 685909 SKin Healthcare by Innovative NanoCAPsuleS
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivs 3.0 Spain
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivs 3.0 Spain
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/vnd.openxmlformats-officedocument.wordprocessingml.document
dc.source.none.fl_str_mv reponame:UPCommons. Portal del coneixement obert de la UPC
instname:Universitat Politècnica de Catalunya (UPC)
instname_str Universitat Politècnica de Catalunya (UPC)
reponame_str UPCommons. Portal del coneixement obert de la UPC
collection UPCommons. Portal del coneixement obert de la UPC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.300719

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