Tyrosinase inactivation in its action on dopa

Under aerobic or anaerobic conditions, tyrosinase undergoes a process of irreversible inactivation induced by its physiological substrate L-dopa. Under aerobic conditions, this inactivation occurs through a process of suicide inactivation involving the form oxy-tyrosinase. Under anaerobic conditions...

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Detalles Bibliográficos
Autores: Muñoz Muñoz, Jose Luis, Acosta Motos, José Ramón, García Molina, Francisco, Varon, Ramon, García Ruíz, Pedro Antonio, Tudela, Jose, García Cánovas, Francisco, Rodríguez López, José Neptuno
Tipo de recurso: artículo
Fecha de publicación:2010
País:España
Institución:Universidad Católica San Antonio de Murcia (UCAM)
Repositorio:RIUCAM. Repositorio Institucional de la Universidad Católica San Antonio de Murcia
OAI Identifier:oai:repositorio.ucam.edu:10952/7323
Acceso en línea:http://hdl.handle.net/10952/7323
https://doi.org/10.1016/j.bbapap.2010.02.015
Access Level:acceso abierto
Palabra clave:o-Diphenol
Stereospecificity
Inactivation
Suicide
Tyrosinase
Descripción
Sumario:Under aerobic or anaerobic conditions, tyrosinase undergoes a process of irreversible inactivation induced by its physiological substrate L-dopa. Under aerobic conditions, this inactivation occurs through a process of suicide inactivation involving the form oxy-tyrosinase. Under anaerobic conditions, both the met- and deoxytyrosinase forms undergo irreversible inactivation. Suicide inactivation in aerobic conditions is slower than the irreversible inactivation under anaerobic conditions. The enzyme has less affinity for the isomer D-dopa than for L-dopa but the velocity of inactivation is the same. We propose mechanisms to explain these processes.