Guidelines for DNA recombination and repair studies: Cellular assays of DNA repair pathways

Understanding the plasticity of genomes has been greatly aided by assays for recombination, repair and mutagenesis. These assays have been developed in microbial systems that provide the advantages of genetic and molecular reporters that can readily be manipulated. Cellular assays comprise genetic,...

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Autores: Klein, Hannah L., Bačinskaja, Giedré, Che, Jun, Cheblal, Anais, Elango, Rajula, Epshtein, Anastasiya, Fitzgerald, Devon M., Gómez González, Belén, Khan, Sharik R., Kumar, Sandeep Mothish, Leland, Bryan A., Marie, Léa, Aguilera López, Andrés
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2019
País:España
Institución:Universidad de Sevilla (US)
Repositorio:idUS. Depósito de Investigación de la Universidad de Sevilla
OAI Identifier:oai:idus.us.es:11441/86449
Acceso en línea:https://hdl.handle.net/11441/86449
https://doi.org/10.15698/mic2019.01.664
Access Level:acceso abierto
Palabra clave:Chromatin dynamics
Chromosome rearrangements
Crossovers
DNA breaks
DNA repair centers
DNA resection
DSBs
Fluorescent proteins
Gene amplification
Gene conversion
Genome instability
Gross chromosome rearrangements
Holliday junctions
Homologous recombination
Mitotic recombination
Mutagenesis
Pulsed field gel electrophoresis
R-loops
Replication fork stalling
Single-particle tracking
Sister chromatid recombination
Sister repetitive sequences
Site-specific chromosome breaks
Toxic recombination intermediates
Yeast artificial chromosome
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spelling Guidelines for DNA recombination and repair studies: Cellular assays of DNA repair pathwaysKlein, Hannah L.Bačinskaja, GiedréChe, JunCheblal, AnaisElango, RajulaEpshtein, AnastasiyaFitzgerald, Devon M.Gómez González, BelénKhan, Sharik R.Kumar, Sandeep MothishLeland, Bryan A.Marie, LéaAguilera López, AndrésChromatin dynamicsChromosome rearrangementsCrossoversDNA breaksDNA repair centersDNA resectionDSBsFluorescent proteinsGene amplificationGene conversionGenome instabilityGross chromosome rearrangementsHolliday junctionsHomologous recombinationMitotic recombinationMutagenesisPulsed field gel electrophoresisR-loopsReplication fork stallingSingle-particle trackingSister chromatid recombinationSister repetitive sequencesSite-specific chromosome breaksToxic recombination intermediatesYeast artificial chromosomeUnderstanding the plasticity of genomes has been greatly aided by assays for recombination, repair and mutagenesis. These assays have been developed in microbial systems that provide the advantages of genetic and molecular reporters that can readily be manipulated. Cellular assays comprise genetic, molecular, and cytological reporters. The assays are powerful tools but each comes with its particular advantages and limitations. Here the most commonly used assays are reviewed, discussed, and presented as the guidelines for future studies.European Research Council ERC2014-ADG669898 TARLOOPMinisterio de Economía y Competitividad BFU2016-75058-PJunta de Andalucía BIO1238Shared Science Publishers OGGenética2019info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttps://hdl.handle.net/11441/86449https://doi.org/10.15698/mic2019.01.664reponame:idUS. Depósito de Investigación de la Universidad de Sevillainstname:Universidad de Sevilla (US)InglésMicrobial Cell, 6 (1), 1-64.ERC2014-ADG669898 TARLOOPBFU2016-75058-PBIO1238http://dx.doi.org/10.15698/mic2019.01.664info:eu-repo/semantics/openAccessoai:idus.us.es:11441/864492026-06-17T12:51:07Z
dc.title.none.fl_str_mv Guidelines for DNA recombination and repair studies: Cellular assays of DNA repair pathways
title Guidelines for DNA recombination and repair studies: Cellular assays of DNA repair pathways
spellingShingle Guidelines for DNA recombination and repair studies: Cellular assays of DNA repair pathways
Klein, Hannah L.
Chromatin dynamics
Chromosome rearrangements
Crossovers
DNA breaks
DNA repair centers
DNA resection
DSBs
Fluorescent proteins
Gene amplification
Gene conversion
Genome instability
Gross chromosome rearrangements
Holliday junctions
Homologous recombination
Mitotic recombination
Mutagenesis
Pulsed field gel electrophoresis
R-loops
Replication fork stalling
Single-particle tracking
Sister chromatid recombination
Sister repetitive sequences
Site-specific chromosome breaks
Toxic recombination intermediates
Yeast artificial chromosome
title_short Guidelines for DNA recombination and repair studies: Cellular assays of DNA repair pathways
title_full Guidelines for DNA recombination and repair studies: Cellular assays of DNA repair pathways
title_fullStr Guidelines for DNA recombination and repair studies: Cellular assays of DNA repair pathways
title_full_unstemmed Guidelines for DNA recombination and repair studies: Cellular assays of DNA repair pathways
title_sort Guidelines for DNA recombination and repair studies: Cellular assays of DNA repair pathways
dc.creator.none.fl_str_mv Klein, Hannah L.
Bačinskaja, Giedré
Che, Jun
Cheblal, Anais
Elango, Rajula
Epshtein, Anastasiya
Fitzgerald, Devon M.
Gómez González, Belén
Khan, Sharik R.
Kumar, Sandeep Mothish
Leland, Bryan A.
Marie, Léa
Aguilera López, Andrés
author Klein, Hannah L.
author_facet Klein, Hannah L.
Bačinskaja, Giedré
Che, Jun
Cheblal, Anais
Elango, Rajula
Epshtein, Anastasiya
Fitzgerald, Devon M.
Gómez González, Belén
Khan, Sharik R.
Kumar, Sandeep Mothish
Leland, Bryan A.
Marie, Léa
Aguilera López, Andrés
author_role author
author2 Bačinskaja, Giedré
Che, Jun
Cheblal, Anais
Elango, Rajula
Epshtein, Anastasiya
Fitzgerald, Devon M.
Gómez González, Belén
Khan, Sharik R.
Kumar, Sandeep Mothish
Leland, Bryan A.
Marie, Léa
Aguilera López, Andrés
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Genética
dc.subject.none.fl_str_mv Chromatin dynamics
Chromosome rearrangements
Crossovers
DNA breaks
DNA repair centers
DNA resection
DSBs
Fluorescent proteins
Gene amplification
Gene conversion
Genome instability
Gross chromosome rearrangements
Holliday junctions
Homologous recombination
Mitotic recombination
Mutagenesis
Pulsed field gel electrophoresis
R-loops
Replication fork stalling
Single-particle tracking
Sister chromatid recombination
Sister repetitive sequences
Site-specific chromosome breaks
Toxic recombination intermediates
Yeast artificial chromosome
topic Chromatin dynamics
Chromosome rearrangements
Crossovers
DNA breaks
DNA repair centers
DNA resection
DSBs
Fluorescent proteins
Gene amplification
Gene conversion
Genome instability
Gross chromosome rearrangements
Holliday junctions
Homologous recombination
Mitotic recombination
Mutagenesis
Pulsed field gel electrophoresis
R-loops
Replication fork stalling
Single-particle tracking
Sister chromatid recombination
Sister repetitive sequences
Site-specific chromosome breaks
Toxic recombination intermediates
Yeast artificial chromosome
description Understanding the plasticity of genomes has been greatly aided by assays for recombination, repair and mutagenesis. These assays have been developed in microbial systems that provide the advantages of genetic and molecular reporters that can readily be manipulated. Cellular assays comprise genetic, molecular, and cytological reporters. The assays are powerful tools but each comes with its particular advantages and limitations. Here the most commonly used assays are reviewed, discussed, and presented as the guidelines for future studies.
publishDate 2019
dc.date.none.fl_str_mv 2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/11441/86449
https://doi.org/10.15698/mic2019.01.664
url https://hdl.handle.net/11441/86449
https://doi.org/10.15698/mic2019.01.664
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Microbial Cell, 6 (1), 1-64.
ERC2014-ADG669898 TARLOOP
BFU2016-75058-P
BIO1238
http://dx.doi.org/10.15698/mic2019.01.664
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Shared Science Publishers OG
publisher.none.fl_str_mv Shared Science Publishers OG
dc.source.none.fl_str_mv reponame:idUS. Depósito de Investigación de la Universidad de Sevilla
instname:Universidad de Sevilla (US)
instname_str Universidad de Sevilla (US)
reponame_str idUS. Depósito de Investigación de la Universidad de Sevilla
collection idUS. Depósito de Investigación de la Universidad de Sevilla
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.301629

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