Guidelines for DNA recombination and repair studies: Cellular assays of DNA repair pathways
Understanding the plasticity of genomes has been greatly aided by assays for recombination, repair and mutagenesis. These assays have been developed in microbial systems that provide the advantages of genetic and molecular reporters that can readily be manipulated. Cellular assays comprise genetic,...
| Autores: | , , , , , , , , , , , , |
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| Formato: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2019 |
| País: | España |
| Recursos: | Universidad de Sevilla (US) |
| Repositorio: | idUS. Depósito de Investigación de la Universidad de Sevilla |
| OAI Identifier: | oai:idus.us.es:11441/86449 |
| Acesso em linha: | https://hdl.handle.net/11441/86449 https://doi.org/10.15698/mic2019.01.664 |
| Access Level: | acceso abierto |
| Palavra-chave: | Chromatin dynamics Chromosome rearrangements Crossovers DNA breaks DNA repair centers DNA resection DSBs Fluorescent proteins Gene amplification Gene conversion Genome instability Gross chromosome rearrangements Holliday junctions Homologous recombination Mitotic recombination Mutagenesis Pulsed field gel electrophoresis R-loops Replication fork stalling Single-particle tracking Sister chromatid recombination Sister repetitive sequences Site-specific chromosome breaks Toxic recombination intermediates Yeast artificial chromosome |
| Resumo: | Understanding the plasticity of genomes has been greatly aided by assays for recombination, repair and mutagenesis. These assays have been developed in microbial systems that provide the advantages of genetic and molecular reporters that can readily be manipulated. Cellular assays comprise genetic, molecular, and cytological reporters. The assays are powerful tools but each comes with its particular advantages and limitations. Here the most commonly used assays are reviewed, discussed, and presented as the guidelines for future studies. |
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