Characterization of Seminal Microbiome of Infertile Idiopathic Patients Using Third-Generation Sequencing Platform

Male fertility; Nanopore sequencing; Seminal microbiota

Detalles Bibliográficos
Autores: Garcia Segura, Sergio, DEL REY AZPIRI, JAVIER, Closa, Laia, Garcia Martínez, Iris, Hobeich Naya, Carlos, Castel, Ana Belén, Vidal Pérez, Francisco
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:11351/9580
Acceso en línea:https://hdl.handle.net/11351/9580
http://hdl.handle.net/11351/9580
Access Level:acceso abierto
Palabra clave:Bacteris
Genètica
Esterilitat
PHENOMENA AND PROCESSES::Microbiological Phenomena::Microbiota
Other subheadings::Other subheadings::Other subheadings::/genetics
DISEASES::Male Urogenital Diseases::Genital Diseases, Male::Infertility
FENÓMENOS Y PROCESOS::fenómenos microbiológicos::microbiota
Otros calificadores::Otros calificadores::Otros calificadores::/genética
ENFERMEDADES::enfermedades urogenitales masculinas::enfermedades de los genitales masculinos::infertilidad
id ES_1597dc2ab6fedcd2bf0765052fbcb16c
oai_identifier_str oai:recercat.cat:11351/9580
network_acronym_str ES
network_name_str España
repository_id_str
dc.title.none.fl_str_mv Characterization of Seminal Microbiome of Infertile Idiopathic Patients Using Third-Generation Sequencing Platform
title Characterization of Seminal Microbiome of Infertile Idiopathic Patients Using Third-Generation Sequencing Platform
spellingShingle Characterization of Seminal Microbiome of Infertile Idiopathic Patients Using Third-Generation Sequencing Platform
Garcia Segura, Sergio
Bacteris
Genètica
Esterilitat
PHENOMENA AND PROCESSES::Microbiological Phenomena::Microbiota
Other subheadings::Other subheadings::Other subheadings::/genetics
DISEASES::Male Urogenital Diseases::Genital Diseases, Male::Infertility
FENÓMENOS Y PROCESOS::fenómenos microbiológicos::microbiota
Otros calificadores::Otros calificadores::Otros calificadores::/genética
ENFERMEDADES::enfermedades urogenitales masculinas::enfermedades de los genitales masculinos::infertilidad
title_short Characterization of Seminal Microbiome of Infertile Idiopathic Patients Using Third-Generation Sequencing Platform
title_full Characterization of Seminal Microbiome of Infertile Idiopathic Patients Using Third-Generation Sequencing Platform
title_fullStr Characterization of Seminal Microbiome of Infertile Idiopathic Patients Using Third-Generation Sequencing Platform
title_full_unstemmed Characterization of Seminal Microbiome of Infertile Idiopathic Patients Using Third-Generation Sequencing Platform
title_sort Characterization of Seminal Microbiome of Infertile Idiopathic Patients Using Third-Generation Sequencing Platform
dc.creator.none.fl_str_mv Garcia Segura, Sergio
DEL REY AZPIRI, JAVIER
Closa, Laia
Garcia Martínez, Iris
Hobeich Naya, Carlos
Castel, Ana Belén
Vidal Pérez, Francisco
author Garcia Segura, Sergio
author_facet Garcia Segura, Sergio
DEL REY AZPIRI, JAVIER
Closa, Laia
Garcia Martínez, Iris
Hobeich Naya, Carlos
Castel, Ana Belén
Vidal Pérez, Francisco
author_role author
author2 DEL REY AZPIRI, JAVIER
Closa, Laia
Garcia Martínez, Iris
Hobeich Naya, Carlos
Castel, Ana Belén
Vidal Pérez, Francisco
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Institut Català de la Salut
[Garcia-Segura S, Del Rey J] Unit of Cell Biology and Medical Genetics, Department of Cell Biology, Physiology and Immunology, Universitat Autònoma de Barcelona, Bellaterra, Spain. [Closa L] Histocompatibility and Immunogenetics Laboratory, Banc de Sang i Teixits (BST), Barcelona, Spain. Medicina Transfusional, Vall d’Hebron Institut de Recerca (VHIR), Universitat Autònoma de Barcelona, Barcelona, Spain. [Garcia-Martínez I, Hobeich C] Grup de Recerca de Medicina Transfusional, Vall d’Hebron Institut de Recerca (VHIR), Barcelona, Spain. Universitat Autònoma de Barcelona, Bellaterra, Spain. Grup de Coagulopaties Congènites, Banc de Sang i Teixits (BST), Barcelona, Spain. [Castel AB] Instituto de Fertilidad, Palma de Mallorca, Spain. [Vidal F] Grup de Recerca de Medicina Transfusional, Vall d’Hebron Institut de Recerca (VHIR), Barcelona, Spain. Universitat Autònoma de Barcelona, Bellaterra, Spain. Grup de Coagulopaties Congènites, Banc de Sang i Teixits (BST), Barcelona, Spain. CIBER de Enfermedades Cardiovasculares (CIBERCV), Madrid, Spain
Vall d'Hebron Barcelona Hospital Campus
dc.subject.none.fl_str_mv Bacteris
Genètica
Esterilitat
PHENOMENA AND PROCESSES::Microbiological Phenomena::Microbiota
Other subheadings::Other subheadings::Other subheadings::/genetics
DISEASES::Male Urogenital Diseases::Genital Diseases, Male::Infertility
FENÓMENOS Y PROCESOS::fenómenos microbiológicos::microbiota
Otros calificadores::Otros calificadores::Otros calificadores::/genética
ENFERMEDADES::enfermedades urogenitales masculinas::enfermedades de los genitales masculinos::infertilidad
topic Bacteris
Genètica
Esterilitat
PHENOMENA AND PROCESSES::Microbiological Phenomena::Microbiota
Other subheadings::Other subheadings::Other subheadings::/genetics
DISEASES::Male Urogenital Diseases::Genital Diseases, Male::Infertility
FENÓMENOS Y PROCESOS::fenómenos microbiológicos::microbiota
Otros calificadores::Otros calificadores::Otros calificadores::/genética
ENFERMEDADES::enfermedades urogenitales masculinas::enfermedades de los genitales masculinos::infertilidad
description Male fertility; Nanopore sequencing; Seminal microbiota
publishDate 2023
dc.date.none.fl_str_mv 2023
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/11351/9580
http://hdl.handle.net/11351/9580
url https://hdl.handle.net/11351/9580
http://hdl.handle.net/11351/9580
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv International Journal of Molecular Sciences;24(9)
https://doi.org/10.3390/ijms24097867
dc.rights.none.fl_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv Scientia
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869403804659613696
spelling Characterization of Seminal Microbiome of Infertile Idiopathic Patients Using Third-Generation Sequencing PlatformGarcia Segura, SergioDEL REY AZPIRI, JAVIERClosa, LaiaGarcia Martínez, IrisHobeich Naya, CarlosCastel, Ana BelénVidal Pérez, FranciscoBacterisGenèticaEsterilitatPHENOMENA AND PROCESSES::Microbiological Phenomena::MicrobiotaOther subheadings::Other subheadings::Other subheadings::/geneticsDISEASES::Male Urogenital Diseases::Genital Diseases, Male::InfertilityFENÓMENOS Y PROCESOS::fenómenos microbiológicos::microbiotaOtros calificadores::Otros calificadores::Otros calificadores::/genéticaENFERMEDADES::enfermedades urogenitales masculinas::enfermedades de los genitales masculinos::infertilidadMale fertility; Nanopore sequencing; Seminal microbiotaFertilitat masculina; Seqüenciació de nanopors; Microbiota seminalFertilidad masculina; Secuenciación de nanoporos; Microbiota seminalSince the first description of a commensal seminal microbiome using sequencing, less than a decade ago, interest in the composition of this microbiome and its relationship with fertility has been growing. Articles using next-generation sequencing techniques agree on the identification of the most abundant bacterial phyla. However, at the genus level, there is still no consensus on which bacteria are most abundant in human seminal plasma. This discrepancy may be due to methodological variability such as sample collection, bacterial DNA extraction methodology, which hypervariable regions of 16S rRNA gene have been amplified, or bioinformatic analysis. In the present work, seminal microbiota of 14 control samples and 42 samples of idiopathic infertile patients were characterized based on full-length sequencing of the 16S rRNA gene using MinION platform from Oxford Nanopore. These same samples had been analyzed previously using Illumina’s MiSeq sequencing platform. Comparison between the results obtained with the two platforms has been used to analyze the impact of sequencing method on the study of the seminal microbiome’s composition. Seminal microbiota observed with MinION were mainly composed of the phyla Firmicutes, Proteobacteria, Bacteroidetes and Actinobacteria, with the most abundant genera being Peptoniphilus, Finegoldia, Staphylococcus, Anaerococcus, Campylobacter, Prevotella, Streptococcus, Lactobacillus, Ezakiella and Enterococcus. This composition was similar to that found by the Illumina platform, since these 10 most abundant genera were also among the most abundant genera detected by the Nanopore platform. In both cases, the top 10 genera represented more than 70% of the classified reads. However, relative abundance of each bacterium did not correlate between these two platforms, with intraindividual variations of up to 50 percentage points in some cases. Results suggest that the effect of the sequencing platform on the characterization of seminal microbiota is not very large at the phylum level, with slightly variances in Firmicutes and Actinobacteria, but presents differences at the genus level. These differences could alter the composition and diversity of bacterial profiles or posterior analyses. This indicates the importance of conducting multi-platform studies to better characterize seminal microbioma.This research was funded by the European Regional Development Fund and Instituto de Salud Carlos III (Economy, Industry and Competitiveness Ministry, Madrid, Spain; Project PI14/00119) and Generalitat de Catalunya (2017SGR1796 and 2021SGR00122).MDPIInstitut Català de la Salut[Garcia-Segura S, Del Rey J] Unit of Cell Biology and Medical Genetics, Department of Cell Biology, Physiology and Immunology, Universitat Autònoma de Barcelona, Bellaterra, Spain. [Closa L] Histocompatibility and Immunogenetics Laboratory, Banc de Sang i Teixits (BST), Barcelona, Spain. Medicina Transfusional, Vall d’Hebron Institut de Recerca (VHIR), Universitat Autònoma de Barcelona, Barcelona, Spain. [Garcia-Martínez I, Hobeich C] Grup de Recerca de Medicina Transfusional, Vall d’Hebron Institut de Recerca (VHIR), Barcelona, Spain. Universitat Autònoma de Barcelona, Bellaterra, Spain. Grup de Coagulopaties Congènites, Banc de Sang i Teixits (BST), Barcelona, Spain. [Castel AB] Instituto de Fertilidad, Palma de Mallorca, Spain. [Vidal F] Grup de Recerca de Medicina Transfusional, Vall d’Hebron Institut de Recerca (VHIR), Barcelona, Spain. Universitat Autònoma de Barcelona, Bellaterra, Spain. Grup de Coagulopaties Congènites, Banc de Sang i Teixits (BST), Barcelona, Spain. CIBER de Enfermedades Cardiovasculares (CIBERCV), Madrid, SpainVall d'Hebron Barcelona Hospital Campus202320232023info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/11351/9580http://hdl.handle.net/11351/9580Scientiareponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésInternational Journal of Molecular Sciences;24(9)https://doi.org/10.3390/ijms24097867Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:11351/95802026-05-29T05:05:01Z
score 15,811543

Similares en LA Referencia