Estrogen-responsive genes in macrophages of the bony fish gilthead seabream: a transcriptomic approach.

The role of sex steroids in the modulation of fish immune responses has received little attention. Previous studies have demonstrated that 17β-estradiol (E2) is able to alter the response of gilthead seabream leukocytes to infectious agents. We have used suppression subtractive hybridization to iden...

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Detalles Bibliográficos
Autores: Liarte, S., Chaves-Pozo, Elena, Abellán-Martínez, Emilia, Meseguer, José, Mulero, Victoriano, Canario, A.V.M., García-Ayala, Alfonsa
Tipo de recurso: artículo
Fecha de publicación:2011
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/313831
Acceso en línea:http://hdl.handle.net/10261/313831
Access Level:acceso abierto
Palabra clave:Centro Oceanográfico de Murcia
Acuicultura
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spelling Estrogen-responsive genes in macrophages of the bony fish gilthead seabream: a transcriptomic approach.Liarte, S.Chaves-Pozo, ElenaAbellán-Martínez, EmiliaMeseguer, JoséMulero, VictorianoCanario, A.V.M.García-Ayala, AlfonsaCentro Oceanográfico de MurciaAcuiculturaThe role of sex steroids in the modulation of fish immune responses has received little attention. Previous studies have demonstrated that 17β-estradiol (E2) is able to alter the response of gilthead seabream leukocytes to infectious agents. We have used suppression subtractive hybridization to identify genes upregulated by E2 (50 ng/ml) in macrophage cultures from gilthead seabream. We isolated 393 up-regulated cDNA fragments that led to the identification of 162 candidate estrogen-responsive genes. Functional analyses revealed the presence of several enriched immune processes and molecular pathways. The E2 up-regulation of some immune-relevant genes was further confirmed by real time RT-PCR. Bioinformatics analysis revealed the ability of E2 to orchestrate profound alterations in the macrophage expression profile, especially immune-related processes and pathways. This is the first report on E2-dependent modifications of fish macrophage transcriptome and lends weight to a suggested role for estrogen in the immune system, the possible significance of which is discussed.202320232011info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501http://hdl.handle.net/10261/313831reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésCentro Oceanográfico de Murciahttps://www.sciencedirect.com/science/article/pii/S0145305X11000759info:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3138312026-05-22T06:33:51Z
dc.title.none.fl_str_mv Estrogen-responsive genes in macrophages of the bony fish gilthead seabream: a transcriptomic approach.
title Estrogen-responsive genes in macrophages of the bony fish gilthead seabream: a transcriptomic approach.
spellingShingle Estrogen-responsive genes in macrophages of the bony fish gilthead seabream: a transcriptomic approach.
Liarte, S.
Centro Oceanográfico de Murcia
Acuicultura
title_short Estrogen-responsive genes in macrophages of the bony fish gilthead seabream: a transcriptomic approach.
title_full Estrogen-responsive genes in macrophages of the bony fish gilthead seabream: a transcriptomic approach.
title_fullStr Estrogen-responsive genes in macrophages of the bony fish gilthead seabream: a transcriptomic approach.
title_full_unstemmed Estrogen-responsive genes in macrophages of the bony fish gilthead seabream: a transcriptomic approach.
title_sort Estrogen-responsive genes in macrophages of the bony fish gilthead seabream: a transcriptomic approach.
dc.creator.none.fl_str_mv Liarte, S.
Chaves-Pozo, Elena
Abellán-Martínez, Emilia
Meseguer, José
Mulero, Victoriano
Canario, A.V.M.
García-Ayala, Alfonsa
author Liarte, S.
author_facet Liarte, S.
Chaves-Pozo, Elena
Abellán-Martínez, Emilia
Meseguer, José
Mulero, Victoriano
Canario, A.V.M.
García-Ayala, Alfonsa
author_role author
author2 Chaves-Pozo, Elena
Abellán-Martínez, Emilia
Meseguer, José
Mulero, Victoriano
Canario, A.V.M.
García-Ayala, Alfonsa
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Centro Oceanográfico de Murcia
Acuicultura
topic Centro Oceanográfico de Murcia
Acuicultura
description The role of sex steroids in the modulation of fish immune responses has received little attention. Previous studies have demonstrated that 17β-estradiol (E2) is able to alter the response of gilthead seabream leukocytes to infectious agents. We have used suppression subtractive hybridization to identify genes upregulated by E2 (50 ng/ml) in macrophage cultures from gilthead seabream. We isolated 393 up-regulated cDNA fragments that led to the identification of 162 candidate estrogen-responsive genes. Functional analyses revealed the presence of several enriched immune processes and molecular pathways. The E2 up-regulation of some immune-relevant genes was further confirmed by real time RT-PCR. Bioinformatics analysis revealed the ability of E2 to orchestrate profound alterations in the macrophage expression profile, especially immune-related processes and pathways. This is the first report on E2-dependent modifications of fish macrophage transcriptome and lends weight to a suggested role for estrogen in the immune system, the possible significance of which is discussed.
publishDate 2011
dc.date.none.fl_str_mv 2011
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/313831
url http://hdl.handle.net/10261/313831
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Centro Oceanográfico de Murcia
https://www.sciencedirect.com/science/article/pii/S0145305X11000759
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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