Estrogen-responsive genes in macrophages of the bony fish gilthead seabream: a transcriptomic approach.
The role of sex steroids in the modulation of fish immune responses has received little attention. Previous studies have demonstrated that 17β-estradiol (E2) is able to alter the response of gilthead seabream leukocytes to infectious agents. We have used suppression subtractive hybridization to iden...
| Autores: | , , , , , , |
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| Tipo de recurso: | artículo |
| Fecha de publicación: | 2011 |
| País: | España |
| Institución: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/313831 |
| Acceso en línea: | http://hdl.handle.net/10261/313831 |
| Access Level: | acceso abierto |
| Palabra clave: | Centro Oceanográfico de Murcia Acuicultura |
| Sumario: | The role of sex steroids in the modulation of fish immune responses has received little attention. Previous studies have demonstrated that 17β-estradiol (E2) is able to alter the response of gilthead seabream leukocytes to infectious agents. We have used suppression subtractive hybridization to identify genes upregulated by E2 (50 ng/ml) in macrophage cultures from gilthead seabream. We isolated 393 up-regulated cDNA fragments that led to the identification of 162 candidate estrogen-responsive genes. Functional analyses revealed the presence of several enriched immune processes and molecular pathways. The E2 up-regulation of some immune-relevant genes was further confirmed by real time RT-PCR. Bioinformatics analysis revealed the ability of E2 to orchestrate profound alterations in the macrophage expression profile, especially immune-related processes and pathways. This is the first report on E2-dependent modifications of fish macrophage transcriptome and lends weight to a suggested role for estrogen in the immune system, the possible significance of which is discussed. |
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