Transcriptomics Analysis Reveals a Putative Role for Hormone Signaling and MADS-Box Genes in Mature Chestnut Shoots Rooting Recalcitrance

20 páginas, 6 figuras

Detalles Bibliográficos
Autores: Vielba, Jesús M., Rico, Saleta, Sevgin, Nevzat, Castro-Camba, Ricardo, Covelo, Purificación, Vidal González, Nieves Pilar, Sánchez Fernández, Conchi
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/286733
Acceso en línea:http://hdl.handle.net/10261/286733
https://api.elsevier.com/content/abstract/scopus_id/85144734562
Access Level:acceso abierto
Palabra clave:Adventitious rooting
Auxin
Chestnut
Mads-box
Maturation
Phytohormones
Recalcitrance
Transcriptomics
id ES_1461a57f8a5580944c19ed30f75fbad1
oai_identifier_str oai:digital.csic.es:10261/286733
network_acronym_str ES
network_name_str España
repository_id_str
spelling Transcriptomics Analysis Reveals a Putative Role for Hormone Signaling and MADS-Box Genes in Mature Chestnut Shoots Rooting RecalcitranceVielba, Jesús M.Rico, SaletaSevgin, NevzatCastro-Camba, RicardoCovelo, PurificaciónVidal González, Nieves PilarSánchez Fernández, ConchiAdventitious rootingAuxinChestnutMads-boxMaturationPhytohormonesRecalcitranceTranscriptomics20 páginas, 6 figurasMaturation imposes several changes in plants, which are particularly drastic in the case of trees. In recalcitrant woody species, such as chestnut (Castanea sativa Mill.), one of the major maturation-related shifts is the loss of the ability to form adventitious roots in response to auxin treatment as the plant ages. To analyze the molecular mechanisms underlying this phenomenon, an in vitro model system of two different lines of microshoots derived from the same field-grown tree was established. While juvenile-like shoots root readily when treated with exogenous auxin, microshoots established from the crown of the tree rarely form roots. In the present study, a transcriptomic analysis was developed to compare the gene expression patterns in both types of shoots 24 h after hormone and wounding treatment, matching the induction phase of the process. Our results support the hypothesis that the inability of adult chestnut tissues to respond to the inductive treatment relies in a deep change of gene expression imposed by maturation that results in a significant transcriptome modification. Differences in phytohormone signaling seem to be the main cause for the recalcitrant behavior of mature shoots, with abscisic acid and ethylene negatively influencing the rooting ability of the chestnut plants. We have identified a set of related MADS-box genes whose expression is modified but not suppressed by the inductive treatment in mature shoots, suggesting a putative link of their activity with the rooting-recalcitrant behavior of this material. Overall, distinct maturation-derived auxin sensibility and homeostasis, and the related modifications in the balance with other phytohormones, seem to govern the outcome of the process in each type of shoots.This work was funded by Xunta de Galicia (Spain) through the projects IN607A and “Contrato Programa” 2021 (AGI/CSIC I + D + I 2021, Ref-ACAM 20210200033), and also by the CSIC program I-COOP + 2020 (RefCOOPB20584).Peer reviewedMultidisciplinary Digital Publishing InstituteXunta de GaliciaConsejo Superior de Investigaciones Científicas (España)Vielba, Jesús M. [0000-0003-1367-535X]Rico, Saleta [0000-0003-2420-8108]Sevgin, Nevzat [0000-0001-5405-060X]Castro-Camba, Ricardo [0000-0003-1525-935X]Covelo, Purificación [0000-0002-0358-6487]Vidal González, Nieves Pilar [0000-0001-8046-3133]Sánchez, Conchi [0000-0002-1304-0736]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202320232022info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/286733https://api.elsevier.com/content/abstract/scopus_id/85144734562reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/GAIN/Axudas para a consolidación e estruturación de unidades de investigación competitivas/IN607Ainfo:eu-repo/grantAgreement/AGI-CSIC/I+D+I 2021/ACAM 20210200033info:eu-repo/grantAgreement/CSIC/I-COOP 2020/COOPB20584Plants (Basel, Switzerland)Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2867332026-05-22T06:33:51Z
dc.title.none.fl_str_mv Transcriptomics Analysis Reveals a Putative Role for Hormone Signaling and MADS-Box Genes in Mature Chestnut Shoots Rooting Recalcitrance
title Transcriptomics Analysis Reveals a Putative Role for Hormone Signaling and MADS-Box Genes in Mature Chestnut Shoots Rooting Recalcitrance
spellingShingle Transcriptomics Analysis Reveals a Putative Role for Hormone Signaling and MADS-Box Genes in Mature Chestnut Shoots Rooting Recalcitrance
Vielba, Jesús M.
Adventitious rooting
Auxin
Chestnut
Mads-box
Maturation
Phytohormones
Recalcitrance
Transcriptomics
title_short Transcriptomics Analysis Reveals a Putative Role for Hormone Signaling and MADS-Box Genes in Mature Chestnut Shoots Rooting Recalcitrance
title_full Transcriptomics Analysis Reveals a Putative Role for Hormone Signaling and MADS-Box Genes in Mature Chestnut Shoots Rooting Recalcitrance
title_fullStr Transcriptomics Analysis Reveals a Putative Role for Hormone Signaling and MADS-Box Genes in Mature Chestnut Shoots Rooting Recalcitrance
title_full_unstemmed Transcriptomics Analysis Reveals a Putative Role for Hormone Signaling and MADS-Box Genes in Mature Chestnut Shoots Rooting Recalcitrance
title_sort Transcriptomics Analysis Reveals a Putative Role for Hormone Signaling and MADS-Box Genes in Mature Chestnut Shoots Rooting Recalcitrance
dc.creator.none.fl_str_mv Vielba, Jesús M.
Rico, Saleta
Sevgin, Nevzat
Castro-Camba, Ricardo
Covelo, Purificación
Vidal González, Nieves Pilar
Sánchez Fernández, Conchi
author Vielba, Jesús M.
author_facet Vielba, Jesús M.
Rico, Saleta
Sevgin, Nevzat
Castro-Camba, Ricardo
Covelo, Purificación
Vidal González, Nieves Pilar
Sánchez Fernández, Conchi
author_role author
author2 Rico, Saleta
Sevgin, Nevzat
Castro-Camba, Ricardo
Covelo, Purificación
Vidal González, Nieves Pilar
Sánchez Fernández, Conchi
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Xunta de Galicia
Consejo Superior de Investigaciones Científicas (España)
Vielba, Jesús M. [0000-0003-1367-535X]
Rico, Saleta [0000-0003-2420-8108]
Sevgin, Nevzat [0000-0001-5405-060X]
Castro-Camba, Ricardo [0000-0003-1525-935X]
Covelo, Purificación [0000-0002-0358-6487]
Vidal González, Nieves Pilar [0000-0001-8046-3133]
Sánchez, Conchi [0000-0002-1304-0736]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Adventitious rooting
Auxin
Chestnut
Mads-box
Maturation
Phytohormones
Recalcitrance
Transcriptomics
topic Adventitious rooting
Auxin
Chestnut
Mads-box
Maturation
Phytohormones
Recalcitrance
Transcriptomics
description 20 páginas, 6 figuras
publishDate 2022
dc.date.none.fl_str_mv 2022
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/286733
https://api.elsevier.com/content/abstract/scopus_id/85144734562
url http://hdl.handle.net/10261/286733
https://api.elsevier.com/content/abstract/scopus_id/85144734562
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/GAIN/Axudas para a consolidación e estruturación de unidades de investigación competitivas/IN607A
info:eu-repo/grantAgreement/AGI-CSIC/I+D+I 2021/ACAM 20210200033
info:eu-repo/grantAgreement/CSIC/I-COOP 2020/COOPB20584
Plants (Basel, Switzerland)
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869403733495906304
score 15,81155

Similares en LA Referencia