Biochemical characteristics of chestnut shoots related to in vitro multiplication and rooting capacities
In vitro shoot cultures from stump sprouts and basal shoots of 15 to 80-year-old chestnut proliferated and rooted with rates higher than those originating from crown material of the same tree. A higher amount of anthocyanins was recorded in basal shoot cultures than in crown cultures. The concentrat...
| Autores: | , , , , |
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| Formato: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 1994 |
| País: | España |
| Recursos: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/196588 |
| Acesso em linha: | http://hdl.handle.net/10261/196588 |
| Access Level: | acceso abierto |
| Palavra-chave: | Auxins Chestnut Cytokinins Juvenility Micropropagation Peroxidase Rooting |
| Resumo: | In vitro shoot cultures from stump sprouts and basal shoots of 15 to 80-year-old chestnut proliferated and rooted with rates higher than those originating from crown material of the same tree. A higher amount of anthocyanins was recorded in basal shoot cultures than in crown cultures. The concentration of N-benzyl-aminopurine (BAP) introduced to the growth medium modified the content of anthocyanins in the shoots grown in vitro. A higher content of endogenous cytokinins was measured in crown shoots than in basal shoots before in vitro establishment. In vitro shoots from two of the three clones examined showed the same relationship at the end of the multiplication cycle. No direct relationship could be found between peroxidase activity (estimated in shoots before in vitro establishment and at the end of multiplication cycle), or the absolute amounts of indole-3-acetic acid (IAA) or the total auxin content (at the end of the multiplication cycle) and the rooting capacities of either type of shoots. There was however a direct relationship between the ratio of IAA/cytokinins and the rooting abilities. |
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