Amperometric thyroxine sensor using a nanocomposite based on graphene modified with gold nanoparticles carrying a thiolated beta-cyclodextrin
This article reports a novel electrochemical sensor based on a nanocomposite for the sensitive determination of Thyroxine (T4), the active form of the hormone. Hydrodynamic amperometry is performed with a nanocomposite electrode based on the dispersion of a graphene–based filler hybrid-nanomaterial...
| Autores: | , , , , , , |
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| Tipo de recurso: | artículo |
| Fecha de publicación: | 2016 |
| País: | España |
| Institución: | Universitat Politècnica de Catalunya (UPC) |
| Repositorio: | UPCommons. Portal del coneixement obert de la UPC |
| Idioma: | inglés |
| OAI Identifier: | oai:upcommons.upc.edu:2117/165258 |
| Acceso en línea: | https://hdl.handle.net/2117/165258 https://dx.doi.org/10.1007/s00604-016-1783-x |
| Access Level: | acceso abierto |
| Palabra clave: | Nanocomposites (Materials) Electrochemical sensors Epoxy resins Nanocomposite Near-percolation composition High-Resolution Transmission Electron Microscopy Biorecognition Carbon filler Epoxy resin Nanocompòsits (Materials) Sensors electroquímics Resines epoxídiques Biorreconeixement Àrees temàtiques de la UPC::Enginyeria dels materials |
| Sumario: | This article reports a novel electrochemical sensor based on a nanocomposite for the sensitive determination of Thyroxine (T4), the active form of the hormone. Hydrodynamic amperometry is performed with a nanocomposite electrode based on the dispersion of a graphene–based filler hybrid-nanomaterial throughout an insulating epoxy resin in the optimum composition ratio (the near–percolation composition). This hybrid-nanomaterial consists of reduced graphene oxide tuned with gold nanoparticles and a biorecognition agent, the thiolated ß-cyclodextrin. Recognition of T4 is accomplished via supramolecular chemistry, due to the formation of an inclusion complex between ß-cyclodextrin and T4. The amperometric device operates at +0.85 V vs. Ag/AgCl, where the oxidation of T4 takes place on the electrode surface. The sensor covers the 1.00 nM to 14 nM T4concentration range in a 0.1 M HCl solution, with a detection limit of 1.00 ± 0.02 nM. The sensor can be easily reset by polishing. It exhibits the lowest detection limit regarding to any other electrochemical electrodes for T4 determination previously described in literature |
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