Mutations related to Antiretroviral Resistance identified by ultra-deep sequencing in HIV-1 infected children under Structured Interruptions of HAART

Although Structured Treatment Interruptions (STI) are currently not considered an alternative strategy for antiretroviral treatment, their true benefits and limitations have not been fully established. Some studies suggest the possibility of improving the quality of life of patients with this strate...

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Authors: Vázquez Guillén, José Manuel, Palacios Saucedo, Gerardo C., Rivera Morales, Lydia G., García Campos, Jorge, Ortiz López, Rocío, Noguera-Julian, Marc|||0000-0002-6194-1395, Paredes, Roger|||0000-0002-6553-691X, Vielma Ramírez, Herlinda J., Ramírez, Teresa, Chávez García, Marcelino, López Guillén, Paulo, Briones Lara, Evangelina, Sánchez Sánchez, Luz M., Vázquez Martínez, Carlos A., Rodríguez Padilla, Cristina
Format: article
Publication Date:2016
Country:España
Institution:Universitat Autònoma de Barcelona
Repository:Dipòsit Digital de Documents de la UAB
Language:English
OAI Identifier:oai:ddd.uab.cat:174694
Online Access:https://ddd.uab.cat/record/174694
https://dx.doi.org/urn:doi:10.1371/journal.pone.0147591
Access Level:Open access
Keyword:SIDA
VIH (Virus)
Antiretroviral resistance
Structured interruptions of HAART
Structured Treatment Interruptions (STI)
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spelling Mutations related to Antiretroviral Resistance identified by ultra-deep sequencing in HIV-1 infected children under Structured Interruptions of HAARTVázquez Guillén, José ManuelPalacios Saucedo, Gerardo C.Rivera Morales, Lydia G.García Campos, JorgeOrtiz López, RocíoNoguera-Julian, Marc|||0000-0002-6194-1395Paredes, Roger|||0000-0002-6553-691XVielma Ramírez, Herlinda J.Ramírez, TeresaChávez García, MarcelinoLópez Guillén, PauloBriones Lara, EvangelinaSánchez Sánchez, Luz M.Vázquez Martínez, Carlos A.Rodríguez Padilla, CristinaSIDAVIH (Virus)Antiretroviral resistanceStructured interruptions of HAARTStructured Treatment Interruptions (STI)Although Structured Treatment Interruptions (STI) are currently not considered an alternative strategy for antiretroviral treatment, their true benefits and limitations have not been fully established. Some studies suggest the possibility of improving the quality of life of patients with this strategy; however, the information that has been obtained corresponds mostly to studies conducted in adults, with a lack of knowledge about its impact on children. Furthermore, mutations associated with antiretroviral resistance could be selected due to sub-therapeutic levels of HAART at each interruption period. Genotyping methods to determine the resistance profiles of the infecting viruses have become increasingly important for the management of patients under STI, thus low-abundance antiretroviral drug-resistant mutations (DRM's) at levels under limit of detection of conventional genotyping (<20% of quasispecies) could increase the risk of virologic failure. In this work, we analyzed the protease and reverse transcriptase regions of the pol gene by ultra-deep sequencing in pediatric patients under STI with the aim of determining the presence of high- and low-abundance DRM's in the viral rebounds generated by the STI. High-abundance mutations in protease and high- and low-abundance mutations in reverse transcriptase were detected but no one of these are directly associated with resistance to antiretroviral drugs. The results could suggest that the evaluated STI program is virologically safe, but strict and carefully planned studies, with greater numbers of patients and interruption/restart cycles, are still needed to evaluate the selection of DRM's during STI. 22016-01-0120162016-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/174694https://dx.doi.org/urn:doi:10.1371/journal.pone.0147591reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengopen accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:1746942026-06-06T12:50:31Z
dc.title.none.fl_str_mv Mutations related to Antiretroviral Resistance identified by ultra-deep sequencing in HIV-1 infected children under Structured Interruptions of HAART
title Mutations related to Antiretroviral Resistance identified by ultra-deep sequencing in HIV-1 infected children under Structured Interruptions of HAART
spellingShingle Mutations related to Antiretroviral Resistance identified by ultra-deep sequencing in HIV-1 infected children under Structured Interruptions of HAART
Vázquez Guillén, José Manuel
SIDA
VIH (Virus)
Antiretroviral resistance
Structured interruptions of HAART
Structured Treatment Interruptions (STI)
title_short Mutations related to Antiretroviral Resistance identified by ultra-deep sequencing in HIV-1 infected children under Structured Interruptions of HAART
title_full Mutations related to Antiretroviral Resistance identified by ultra-deep sequencing in HIV-1 infected children under Structured Interruptions of HAART
title_fullStr Mutations related to Antiretroviral Resistance identified by ultra-deep sequencing in HIV-1 infected children under Structured Interruptions of HAART
title_full_unstemmed Mutations related to Antiretroviral Resistance identified by ultra-deep sequencing in HIV-1 infected children under Structured Interruptions of HAART
title_sort Mutations related to Antiretroviral Resistance identified by ultra-deep sequencing in HIV-1 infected children under Structured Interruptions of HAART
dc.creator.none.fl_str_mv Vázquez Guillén, José Manuel
Palacios Saucedo, Gerardo C.
Rivera Morales, Lydia G.
García Campos, Jorge
Ortiz López, Rocío
Noguera-Julian, Marc|||0000-0002-6194-1395
Paredes, Roger|||0000-0002-6553-691X
Vielma Ramírez, Herlinda J.
Ramírez, Teresa
Chávez García, Marcelino
López Guillén, Paulo
Briones Lara, Evangelina
Sánchez Sánchez, Luz M.
Vázquez Martínez, Carlos A.
Rodríguez Padilla, Cristina
author Vázquez Guillén, José Manuel
author_facet Vázquez Guillén, José Manuel
Palacios Saucedo, Gerardo C.
Rivera Morales, Lydia G.
García Campos, Jorge
Ortiz López, Rocío
Noguera-Julian, Marc|||0000-0002-6194-1395
Paredes, Roger|||0000-0002-6553-691X
Vielma Ramírez, Herlinda J.
Ramírez, Teresa
Chávez García, Marcelino
López Guillén, Paulo
Briones Lara, Evangelina
Sánchez Sánchez, Luz M.
Vázquez Martínez, Carlos A.
Rodríguez Padilla, Cristina
author_role author
author2 Palacios Saucedo, Gerardo C.
Rivera Morales, Lydia G.
García Campos, Jorge
Ortiz López, Rocío
Noguera-Julian, Marc|||0000-0002-6194-1395
Paredes, Roger|||0000-0002-6553-691X
Vielma Ramírez, Herlinda J.
Ramírez, Teresa
Chávez García, Marcelino
López Guillén, Paulo
Briones Lara, Evangelina
Sánchez Sánchez, Luz M.
Vázquez Martínez, Carlos A.
Rodríguez Padilla, Cristina
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv SIDA
VIH (Virus)
Antiretroviral resistance
Structured interruptions of HAART
Structured Treatment Interruptions (STI)
topic SIDA
VIH (Virus)
Antiretroviral resistance
Structured interruptions of HAART
Structured Treatment Interruptions (STI)
description Although Structured Treatment Interruptions (STI) are currently not considered an alternative strategy for antiretroviral treatment, their true benefits and limitations have not been fully established. Some studies suggest the possibility of improving the quality of life of patients with this strategy; however, the information that has been obtained corresponds mostly to studies conducted in adults, with a lack of knowledge about its impact on children. Furthermore, mutations associated with antiretroviral resistance could be selected due to sub-therapeutic levels of HAART at each interruption period. Genotyping methods to determine the resistance profiles of the infecting viruses have become increasingly important for the management of patients under STI, thus low-abundance antiretroviral drug-resistant mutations (DRM's) at levels under limit of detection of conventional genotyping (<20% of quasispecies) could increase the risk of virologic failure. In this work, we analyzed the protease and reverse transcriptase regions of the pol gene by ultra-deep sequencing in pediatric patients under STI with the aim of determining the presence of high- and low-abundance DRM's in the viral rebounds generated by the STI. High-abundance mutations in protease and high- and low-abundance mutations in reverse transcriptase were detected but no one of these are directly associated with resistance to antiretroviral drugs. The results could suggest that the evaluated STI program is virologically safe, but strict and carefully planned studies, with greater numbers of patients and interruption/restart cycles, are still needed to evaluate the selection of DRM's during STI.
publishDate 2016
dc.date.none.fl_str_mv 2
2016-01-01
2016
2016-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/174694
https://dx.doi.org/urn:doi:10.1371/journal.pone.0147591
url https://ddd.uab.cat/record/174694
https://dx.doi.org/urn:doi:10.1371/journal.pone.0147591
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
collection Dipòsit Digital de Documents de la UAB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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