Reactivation of ancestral strains of HIV-1 in the gp120 V3 env region in patients failing antiretroviral therapy and subjected to structured treatment interruption

We analyzed gp120V3 HIV-1 env region genetic diversity of 27 patients failing antiretrovirals and subjected to 12-week structured treatment interruption (STI). Based on heteroduplex mobility assays, eight patients presented low pre- and post-STI genetic diversity (G1); five presented high pre-STI bu...

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Detalhes bibliográficos
Autores: Silva, Wilson Pereira [UNIFESP], Santos, Domingos Ezenildo Matos dos [UNIFESP], Leal, Elcio de Souza [UNIFESP], Brunstein, Adriana [UNIFESP], Sucupira, Maria Cecília Araripe [UNIFESP], Sabino, Ester Cerdeira [UNIFESP], Diaz, Ricardo Sobhie [UNIFESP]
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2006
País:Brasil
Recursos:Universidade Federal de São Paulo (UNIFESP)
Repositorio:Repositório Institucional da UNIFESP
Idioma:inglés
OAI Identifier:oai:repositorio.unifesp.br:11600/29208
Acesso em linha:http://dx.doi.org/10.1016/j.virol.2006.04.039
http://repositorio.unifesp.br/handle/11600/29208
Access Level:acceso abierto
Palavra-chave:structured treatment interruption
HIV-1
envelope gene
antiretroviral resistance
Descrição
Resumo:We analyzed gp120V3 HIV-1 env region genetic diversity of 27 patients failing antiretrovirals and subjected to 12-week structured treatment interruption (STI). Based on heteroduplex mobility assays, eight patients presented low pre- and post-STI genetic diversity (G1); five presented high pre-STI but low post-STI diversity (G2); five presented low pre-STI and high post-STI diversity (G3); and nine, high pre- and post-STI diversity (G4). One patient from G1, two from G2 and two from G3 were subjected to proviral DNA end-point PCR and sequencing. in three patients, the dramatic disturbance caused by STI resulted in ancestral viral progeny activation, which repopulated the cell reservoir. in two patients presenting highly homogeneous sequences and low immune selective pressure (dN/dS ratio < 1), this phenomenon was not observed. the mechanisms involved in viral evolution, in which antiretroviral therapy also applies selective pressure, sometimes affects coreceptor usage of circulating viruses, leading to the suppression of x4 strains. (c) 2006 Published by Elsevier Inc.

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