A method to estimate endogenous losses of nitrogen and amino acids at the ileal level in growing rabbits
[EN] Apparent ileal digestibility can be corrected to give the true ileal digestibility of the nitrogen and amino acids provided by the diet, by determining the flow of endogenous nitrogen and amino acids (from desquamated epithelial cells of gastrointestinal mucosa, mucins and digestive enzymes). T...
| Autores: | , , , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Fecha de publicación: | 2024 |
| País: | España |
| Institución: | Universitat Politècnica de València (UPV) |
| Repositorio: | RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia |
| Idioma: | inglés |
| OAI Identifier: | oai:riunet.upv.es:10251/203699 |
| Acceso en línea: | https://riunet.upv.es/handle/10251/203699 |
| Access Level: | acceso abierto |
| Palabra clave: | Ileal Nitrogen Amino acids Endogenous losses Growing rabbit |
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A method to estimate endogenous losses of nitrogen and amino acids at the ileal level in growing rabbitsMarín-García, Pablo JesúsRodríguez, MireiaRódenas, LuísLópez-Luján, María del CarmenBlas, EnriqueMoya, V.J.|||0000-0002-6769-0515Martinez-Paredes, Eugenio|||0000-0002-0631-1032Cambra López, María|||0000-0001-6130-9392Pascual Amorós, Juan José|||0000-0002-7224-9978IlealNitrogenAmino acidsEndogenous lossesGrowing rabbit[EN] Apparent ileal digestibility can be corrected to give the true ileal digestibility of the nitrogen and amino acids provided by the diet, by determining the flow of endogenous nitrogen and amino acids (from desquamated epithelial cells of gastrointestinal mucosa, mucins and digestive enzymes). This flow of nitrogen and amino acids has been studied in adult rabbits fitted with a T-cannula, but remains unknown for growing rabbits. The aim of this work was to propose a method to estimate endogenous nitrogen and amino acid losses in the ileum of growing rabbits slaughtered at 64 d of age from 20:00 h. For this purpose, two experiments were carried out. The first was performed with 10 weaned rabbits fed with a diet with casein as the only source of protein (whose ileal digestibility is 100%) and labelled with ytterbium. This experiment allowed us to identify the relationship between the ileal flow of endogenous nitrogen (IFEN) and the dry matter intake in the last 24 h before slaughter (DMI), which fits the equation: IFEN (mg/d)=5.99 DMI (g/d) +133; (R2=0.778, residual standard deviation=138, P<0.001, n=10). The second experiment was carried out with 36 rabbits fed the same diet but without ytterbium, with whose ileal content 9 pools were constituted to determine the amino acid profile of endogenous nitrogen, which was found to be rich in glutamic acid, serine, aspartic acid, glycine, valine and threonine (15.97±1.33; 8.00±0.80; 7.06±0.72; 6.24±0.77; 5.48±0.51 and 4.97±0.47 g/16 g of N, respectively) and poor in methionine and histidine (1.05±0.06 and 1.34±0.16 g/16 g of N, respectively). Knowing the DMI of a certain growing rabbit in the 24 h prior to slaughter, the combined use of the equation and the amino acid profile obtained makes it possible to estimate the ileal endogenous losses of each amino acid.Conselleria de Innovación, Universidades, Ciencia y Sociedad Digital of the Generalitat Valenciana (AICO/2021/256)Universitat Politècnica de ValènciaDepartamento de Ciencia AnimalInstituto Universitario de Ciencia y Tecnología AnimalEscuela Técnica Superior de Ingeniería Agronómica y del Medio NaturalConselleria de Innovación, Universidades, Ciencia y Sociedad Digital, Generalitat ValencianaRepositorio Institucional de la Universitat Politècnica de València Riunet20242024-03-28journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://riunet.upv.es/handle/10251/203699reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valénciainstname:Universitat Politècnica de València (UPV)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Reconocimiento - No comercial - Compartir igual (by-nc-sa) http://creativecommons.org/licenses/by-nc-sa/4.0/info:eu-repo/semantics/openAccessoai:riunet.upv.es:10251/2036992026-06-13T07:49:27Z |
| dc.title.none.fl_str_mv |
A method to estimate endogenous losses of nitrogen and amino acids at the ileal level in growing rabbits |
| title |
A method to estimate endogenous losses of nitrogen and amino acids at the ileal level in growing rabbits |
| spellingShingle |
A method to estimate endogenous losses of nitrogen and amino acids at the ileal level in growing rabbits Marín-García, Pablo Jesús Ileal Nitrogen Amino acids Endogenous losses Growing rabbit |
| title_short |
A method to estimate endogenous losses of nitrogen and amino acids at the ileal level in growing rabbits |
| title_full |
A method to estimate endogenous losses of nitrogen and amino acids at the ileal level in growing rabbits |
| title_fullStr |
A method to estimate endogenous losses of nitrogen and amino acids at the ileal level in growing rabbits |
| title_full_unstemmed |
A method to estimate endogenous losses of nitrogen and amino acids at the ileal level in growing rabbits |
| title_sort |
A method to estimate endogenous losses of nitrogen and amino acids at the ileal level in growing rabbits |
| dc.creator.none.fl_str_mv |
Marín-García, Pablo Jesús Rodríguez, Mireia Ródenas, Luís López-Luján, María del Carmen Blas, Enrique Moya, V.J.|||0000-0002-6769-0515 Martinez-Paredes, Eugenio|||0000-0002-0631-1032 Cambra López, María|||0000-0001-6130-9392 Pascual Amorós, Juan José|||0000-0002-7224-9978 |
| author |
Marín-García, Pablo Jesús |
| author_facet |
Marín-García, Pablo Jesús Rodríguez, Mireia Ródenas, Luís López-Luján, María del Carmen Blas, Enrique Moya, V.J.|||0000-0002-6769-0515 Martinez-Paredes, Eugenio|||0000-0002-0631-1032 Cambra López, María|||0000-0001-6130-9392 Pascual Amorós, Juan José|||0000-0002-7224-9978 |
| author_role |
author |
| author2 |
Rodríguez, Mireia Ródenas, Luís López-Luján, María del Carmen Blas, Enrique Moya, V.J.|||0000-0002-6769-0515 Martinez-Paredes, Eugenio|||0000-0002-0631-1032 Cambra López, María|||0000-0001-6130-9392 Pascual Amorós, Juan José|||0000-0002-7224-9978 |
| author2_role |
author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Departamento de Ciencia Animal Instituto Universitario de Ciencia y Tecnología Animal Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural Conselleria de Innovación, Universidades, Ciencia y Sociedad Digital, Generalitat Valenciana Repositorio Institucional de la Universitat Politècnica de València Riunet |
| dc.subject.none.fl_str_mv |
Ileal Nitrogen Amino acids Endogenous losses Growing rabbit |
| topic |
Ileal Nitrogen Amino acids Endogenous losses Growing rabbit |
| description |
[EN] Apparent ileal digestibility can be corrected to give the true ileal digestibility of the nitrogen and amino acids provided by the diet, by determining the flow of endogenous nitrogen and amino acids (from desquamated epithelial cells of gastrointestinal mucosa, mucins and digestive enzymes). This flow of nitrogen and amino acids has been studied in adult rabbits fitted with a T-cannula, but remains unknown for growing rabbits. The aim of this work was to propose a method to estimate endogenous nitrogen and amino acid losses in the ileum of growing rabbits slaughtered at 64 d of age from 20:00 h. For this purpose, two experiments were carried out. The first was performed with 10 weaned rabbits fed with a diet with casein as the only source of protein (whose ileal digestibility is 100%) and labelled with ytterbium. This experiment allowed us to identify the relationship between the ileal flow of endogenous nitrogen (IFEN) and the dry matter intake in the last 24 h before slaughter (DMI), which fits the equation: IFEN (mg/d)=5.99 DMI (g/d) +133; (R2=0.778, residual standard deviation=138, P<0.001, n=10). The second experiment was carried out with 36 rabbits fed the same diet but without ytterbium, with whose ileal content 9 pools were constituted to determine the amino acid profile of endogenous nitrogen, which was found to be rich in glutamic acid, serine, aspartic acid, glycine, valine and threonine (15.97±1.33; 8.00±0.80; 7.06±0.72; 6.24±0.77; 5.48±0.51 and 4.97±0.47 g/16 g of N, respectively) and poor in methionine and histidine (1.05±0.06 and 1.34±0.16 g/16 g of N, respectively). Knowing the DMI of a certain growing rabbit in the 24 h prior to slaughter, the combined use of the equation and the amino acid profile obtained makes it possible to estimate the ileal endogenous losses of each amino acid. |
| publishDate |
2024 |
| dc.date.none.fl_str_mv |
2024 2024-03-28 |
| dc.type.none.fl_str_mv |
journal article http://purl.org/coar/resource_type/c_6501 VoR http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| dc.type.openaire.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| dc.identifier.none.fl_str_mv |
https://riunet.upv.es/handle/10251/203699 |
| url |
https://riunet.upv.es/handle/10251/203699 |
| dc.language.none.fl_str_mv |
Inglés eng |
| language_invalid_str_mv |
Inglés |
| language |
eng |
| dc.rights.none.fl_str_mv |
open access http://purl.org/coar/access_right/c_abf2 Reconocimiento - No comercial - Compartir igual (by-nc-sa) http://creativecommons.org/licenses/by-nc-sa/4.0/ |
| dc.rights.openaire.fl_str_mv |
info:eu-repo/semantics/openAccess |
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open access http://purl.org/coar/access_right/c_abf2 Reconocimiento - No comercial - Compartir igual (by-nc-sa) http://creativecommons.org/licenses/by-nc-sa/4.0/ |
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openAccess |
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application/pdf |
| dc.publisher.none.fl_str_mv |
Universitat Politècnica de València |
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Universitat Politècnica de València |
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reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia instname:Universitat Politècnica de València (UPV) |
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Universitat Politècnica de València (UPV) |
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RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia |
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RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia |
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