Cell-based high-throughput screening assay for identification of G-protein-coupled receptors agonists and antagonists

The GPCRs are the target of a significant portion of all approved drugs and also represent an important parcel of potential drug targets for new indications. Activation of GPCRs can be detected by the utilization of functional cell-based assays like measuring transient intracellular calcium mobiliza...

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Detalles Bibliográficos
Autor: Bejarano Prudencio, Alberto
Tipo de recurso: artículo
Fecha de publicación:2013
País:España
Institución:Universidad de Alcalá (UAH)
Repositorio:e_Buah Biblioteca Digital Universidad de Alcalá
Idioma:inglés
OAI Identifier:oai:ebuah.uah.es:10017/15202
Acceso en línea:http://hdl.handle.net/10017/15202
Access Level:acceso abierto
Palabra clave:Ca2+ mobilization
GPCRs
FLIPR
Fluorescent Ca2+ dye
High-throughput screening
Biología y Biomedicina
Farmacia
Biology
Pharmacy
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spelling Cell-based high-throughput screening assay for identification of G-protein-coupled receptors agonists and antagonistsBejarano Prudencio, AlbertoCa2+ mobilizationGPCRsFLIPRFluorescent Ca2+ dyeHigh-throughput screeningBiología y BiomedicinaFarmaciaBiologyPharmacyThe GPCRs are the target of a significant portion of all approved drugs and also represent an important parcel of potential drug targets for new indications. Activation of GPCRs can be detected by the utilization of functional cell-based assays like measuring transient intracellular calcium mobilization. The emergence of the fluorescent imaging plate reader and Ca2+-sensitive fluorescent dyes has made the high-content screening of GPCR possible. Here was applied a dual automated FLIPR platform to testing a large collection of compounds to identification of both agonist and antagonists form a single screen of GPCRInnovARTE, Grupo Docente. Universidad de Alcalá20132013-01-01journal articlehttp://purl.org/coar/resource_type/c_6501NAhttp://purl.org/coar/version/c_be7fb7dd8ff6fe43info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10017/15202reponame:e_Buah Biblioteca Digital Universidad de Alcaláinstname:Universidad de Alcalá (UAH)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:ebuah.uah.es:10017/152022026-06-18T11:13:07Z
dc.title.none.fl_str_mv Cell-based high-throughput screening assay for identification of G-protein-coupled receptors agonists and antagonists
title Cell-based high-throughput screening assay for identification of G-protein-coupled receptors agonists and antagonists
spellingShingle Cell-based high-throughput screening assay for identification of G-protein-coupled receptors agonists and antagonists
Bejarano Prudencio, Alberto
Ca2+ mobilization
GPCRs
FLIPR
Fluorescent Ca2+ dye
High-throughput screening
Biología y Biomedicina
Farmacia
Biology
Pharmacy
title_short Cell-based high-throughput screening assay for identification of G-protein-coupled receptors agonists and antagonists
title_full Cell-based high-throughput screening assay for identification of G-protein-coupled receptors agonists and antagonists
title_fullStr Cell-based high-throughput screening assay for identification of G-protein-coupled receptors agonists and antagonists
title_full_unstemmed Cell-based high-throughput screening assay for identification of G-protein-coupled receptors agonists and antagonists
title_sort Cell-based high-throughput screening assay for identification of G-protein-coupled receptors agonists and antagonists
dc.creator.none.fl_str_mv Bejarano Prudencio, Alberto
author Bejarano Prudencio, Alberto
author_facet Bejarano Prudencio, Alberto
author_role author
dc.subject.none.fl_str_mv Ca2+ mobilization
GPCRs
FLIPR
Fluorescent Ca2+ dye
High-throughput screening
Biología y Biomedicina
Farmacia
Biology
Pharmacy
topic Ca2+ mobilization
GPCRs
FLIPR
Fluorescent Ca2+ dye
High-throughput screening
Biología y Biomedicina
Farmacia
Biology
Pharmacy
description The GPCRs are the target of a significant portion of all approved drugs and also represent an important parcel of potential drug targets for new indications. Activation of GPCRs can be detected by the utilization of functional cell-based assays like measuring transient intracellular calcium mobilization. The emergence of the fluorescent imaging plate reader and Ca2+-sensitive fluorescent dyes has made the high-content screening of GPCR possible. Here was applied a dual automated FLIPR platform to testing a large collection of compounds to identification of both agonist and antagonists form a single screen of GPCR
publishDate 2013
dc.date.none.fl_str_mv 2013
2013-01-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
NA
http://purl.org/coar/version/c_be7fb7dd8ff6fe43
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10017/15202
url http://hdl.handle.net/10017/15202
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv InnovARTE, Grupo Docente. Universidad de Alcalá
publisher.none.fl_str_mv InnovARTE, Grupo Docente. Universidad de Alcalá
dc.source.none.fl_str_mv reponame:e_Buah Biblioteca Digital Universidad de Alcalá
instname:Universidad de Alcalá (UAH)
instname_str Universidad de Alcalá (UAH)
reponame_str e_Buah Biblioteca Digital Universidad de Alcalá
collection e_Buah Biblioteca Digital Universidad de Alcalá
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.301603

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