Cell-based high-throughput screening assay for identification of G-protein-coupled receptors agonists and antagonists
The GPCRs are the target of a significant portion of all approved drugs and also represent an important parcel of potential drug targets for new indications. Activation of GPCRs can be detected by the utilization of functional cell-based assays like measuring transient intracellular calcium mobiliza...
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| Tipo de recurso: | artículo |
| Fecha de publicación: | 2013 |
| País: | España |
| Institución: | Universidad de Alcalá (UAH) |
| Repositorio: | e_Buah Biblioteca Digital Universidad de Alcalá |
| Idioma: | inglés |
| OAI Identifier: | oai:ebuah.uah.es:10017/15202 |
| Acceso en línea: | http://hdl.handle.net/10017/15202 |
| Access Level: | acceso abierto |
| Palabra clave: | Ca2+ mobilization GPCRs FLIPR Fluorescent Ca2+ dye High-throughput screening Biología y Biomedicina Farmacia Biology Pharmacy |
| Sumario: | The GPCRs are the target of a significant portion of all approved drugs and also represent an important parcel of potential drug targets for new indications. Activation of GPCRs can be detected by the utilization of functional cell-based assays like measuring transient intracellular calcium mobilization. The emergence of the fluorescent imaging plate reader and Ca2+-sensitive fluorescent dyes has made the high-content screening of GPCR possible. Here was applied a dual automated FLIPR platform to testing a large collection of compounds to identification of both agonist and antagonists form a single screen of GPCR |
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