Fully Compatible and Ultra-Sensitive Micellar Electrokinetic Chromatography-Tandem Mass Spectrometry Using Sheathless Porous-Tip Interfacing

The on-line coupling of micellar electrokinetic chromatography and mass spectrometry (MEKC-MS) is often hampered by incompatibility problems leading to reduced separation performance and unfavorable limits of detection (LODs). Here we propose a new selective and highly sensitive MEKC-MS/MS method em...

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Autores: Moreno-González, David, Haselberg, Rob, Gámiz-Gracia, Laura, García-Campaña, Ana M., de Jong, Gerhardus J., Somsen, Govert W.
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2017
País:España
Institución:Universidad de Jaén
Repositorio:RUJA. Repositorio Institucional de la Producción Científica de la Universidad de Jaén
OAI Identifier:oai:ruja.ujaen.es:10953/3247
Acceso en línea:https://www.sciencedirect.com/science/article/pii/S0021967317314565
https://hdl.handle.net/10953/3247
Access Level:acceso abierto
Palabra clave:Volatile surfactant
Carbamates
Micellar electrokinetic chromatography
Mass spectrometry
Sheathless interfacing
Electrospray ionization
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spelling Fully Compatible and Ultra-Sensitive Micellar Electrokinetic Chromatography-Tandem Mass Spectrometry Using Sheathless Porous-Tip InterfacingMoreno-González, DavidHaselberg, RobGámiz-Gracia, LauraGarcía-Campaña, Ana M.de Jong, Gerhardus J.Somsen, Govert W.Volatile surfactantCarbamatesMicellar electrokinetic chromatographyMass spectrometrySheathless interfacingElectrospray ionizationThe on-line coupling of micellar electrokinetic chromatography and mass spectrometry (MEKC-MS) is often hampered by incompatibility problems leading to reduced separation performance and unfavorable limits of detection (LODs). Here we propose a new selective and highly sensitive MEKC-MS/MS method employing a sheathless porous-tip interface in combination with a micellar phase comprised of semi-volatile surfactant molecules. Carbamate pesticides (CRBs) were selected as representative model compounds being neutral toxic pollutants potentially present at trace levels in environmental water samples. A background electrolyte of 75 mM perfluorooctanoic acid adjusted to pH 9.0 with ammonium hydroxide allowed efficient separation of 15 CRBs and appeared fully compatible with electrospray ionization (ESI)-MS. Interfacing parameters, such as the distance between the capillary tip and mass-spectrometer inlet, ESI voltage, and dry gas temperature and flow were optimized in order to attain good spray stability and high analyte signal-to-noise ratios. For CRBs the LODs ranged from 0.2 to 3.9 ng L−1 (13 nL injected, i.e., 2% of capillary volume), representing an improvement for certain CRBs of more than 300-fold when compared with conventional sheath-liquid interfacing. Good linearity (R2 > 0.99) and satisfactory reproducibility were obtained for all CRBs with interday RSD values for peak area and migration time of 4.0–11.3% and below 1.5%, respectively. Analysis of spiked mineral water showed that the new MEKC-MS/MS method allows selective and quantitative determination of CRB concentrations below the maximum residue limit of 100 ng L−1 without the need for sample preconcentration.Elsevier202420242017info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionapplication/mswordhttps://www.sciencedirect.com/science/article/pii/S0021967317314565https://hdl.handle.net/10953/3247reponame:RUJA. Repositorio Institucional de la Producción Científica de la Universidad de Jaéninstname:Universidad de JaénInglésJournal of Chromatography A Volume 1524, Pages 283 – 289Atribución-NoComercial-SinDerivadas 3.0 Españahttp://creativecommons.org/licenses/by-nc-nd/3.0/es/info:eu-repo/semantics/openAccessoai:ruja.ujaen.es:10953/32472026-06-24T12:41:07Z
dc.title.none.fl_str_mv Fully Compatible and Ultra-Sensitive Micellar Electrokinetic Chromatography-Tandem Mass Spectrometry Using Sheathless Porous-Tip Interfacing
title Fully Compatible and Ultra-Sensitive Micellar Electrokinetic Chromatography-Tandem Mass Spectrometry Using Sheathless Porous-Tip Interfacing
spellingShingle Fully Compatible and Ultra-Sensitive Micellar Electrokinetic Chromatography-Tandem Mass Spectrometry Using Sheathless Porous-Tip Interfacing
Moreno-González, David
Volatile surfactant
Carbamates
Micellar electrokinetic chromatography
Mass spectrometry
Sheathless interfacing
Electrospray ionization
title_short Fully Compatible and Ultra-Sensitive Micellar Electrokinetic Chromatography-Tandem Mass Spectrometry Using Sheathless Porous-Tip Interfacing
title_full Fully Compatible and Ultra-Sensitive Micellar Electrokinetic Chromatography-Tandem Mass Spectrometry Using Sheathless Porous-Tip Interfacing
title_fullStr Fully Compatible and Ultra-Sensitive Micellar Electrokinetic Chromatography-Tandem Mass Spectrometry Using Sheathless Porous-Tip Interfacing
title_full_unstemmed Fully Compatible and Ultra-Sensitive Micellar Electrokinetic Chromatography-Tandem Mass Spectrometry Using Sheathless Porous-Tip Interfacing
title_sort Fully Compatible and Ultra-Sensitive Micellar Electrokinetic Chromatography-Tandem Mass Spectrometry Using Sheathless Porous-Tip Interfacing
dc.creator.none.fl_str_mv Moreno-González, David
Haselberg, Rob
Gámiz-Gracia, Laura
García-Campaña, Ana M.
de Jong, Gerhardus J.
Somsen, Govert W.
author Moreno-González, David
author_facet Moreno-González, David
Haselberg, Rob
Gámiz-Gracia, Laura
García-Campaña, Ana M.
de Jong, Gerhardus J.
Somsen, Govert W.
author_role author
author2 Haselberg, Rob
Gámiz-Gracia, Laura
García-Campaña, Ana M.
de Jong, Gerhardus J.
Somsen, Govert W.
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Volatile surfactant
Carbamates
Micellar electrokinetic chromatography
Mass spectrometry
Sheathless interfacing
Electrospray ionization
topic Volatile surfactant
Carbamates
Micellar electrokinetic chromatography
Mass spectrometry
Sheathless interfacing
Electrospray ionization
description The on-line coupling of micellar electrokinetic chromatography and mass spectrometry (MEKC-MS) is often hampered by incompatibility problems leading to reduced separation performance and unfavorable limits of detection (LODs). Here we propose a new selective and highly sensitive MEKC-MS/MS method employing a sheathless porous-tip interface in combination with a micellar phase comprised of semi-volatile surfactant molecules. Carbamate pesticides (CRBs) were selected as representative model compounds being neutral toxic pollutants potentially present at trace levels in environmental water samples. A background electrolyte of 75 mM perfluorooctanoic acid adjusted to pH 9.0 with ammonium hydroxide allowed efficient separation of 15 CRBs and appeared fully compatible with electrospray ionization (ESI)-MS. Interfacing parameters, such as the distance between the capillary tip and mass-spectrometer inlet, ESI voltage, and dry gas temperature and flow were optimized in order to attain good spray stability and high analyte signal-to-noise ratios. For CRBs the LODs ranged from 0.2 to 3.9 ng L−1 (13 nL injected, i.e., 2% of capillary volume), representing an improvement for certain CRBs of more than 300-fold when compared with conventional sheath-liquid interfacing. Good linearity (R2 > 0.99) and satisfactory reproducibility were obtained for all CRBs with interday RSD values for peak area and migration time of 4.0–11.3% and below 1.5%, respectively. Analysis of spiked mineral water showed that the new MEKC-MS/MS method allows selective and quantitative determination of CRB concentrations below the maximum residue limit of 100 ng L−1 without the need for sample preconcentration.
publishDate 2017
dc.date.none.fl_str_mv 2017
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://www.sciencedirect.com/science/article/pii/S0021967317314565
https://hdl.handle.net/10953/3247
url https://www.sciencedirect.com/science/article/pii/S0021967317314565
https://hdl.handle.net/10953/3247
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Journal of Chromatography A Volume 1524, Pages 283 – 289
dc.rights.none.fl_str_mv Atribución-NoComercial-SinDerivadas 3.0 España
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Atribución-NoComercial-SinDerivadas 3.0 España
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/msword
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:RUJA. Repositorio Institucional de la Producción Científica de la Universidad de Jaén
instname:Universidad de Jaén
instname_str Universidad de Jaén
reponame_str RUJA. Repositorio Institucional de la Producción Científica de la Universidad de Jaén
collection RUJA. Repositorio Institucional de la Producción Científica de la Universidad de Jaén
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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