Expression of oncogenic drivers in 3D cell culture depends on nuclear ATP synthesis by NUDT5

The experimental work mentioned was supported by European Research Council (Project “4D Genome” 609989), the Ministerio de Economía y Competitividad (Project G62426937) and the Generalitat de Catalunya (Project AGAUR SGR 575).

Detalles Bibliográficos
Autores: Pickup, Katherine E., Pardow, Felicitas, Carbonell-Caballero, José, Lioutas, Antonios, Villanueva-Cañas, José Luis, Wright, Roni, Beato, Miguel
Tipo de recurso: artículo
Fecha de publicación:2019
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:20.500.12328/3019
Acceso en línea:http://hdl.handle.net/20.500.12328/3019
https://dx.doi.org/10.3390/cancers11091337
Access Level:acceso abierto
Palabra clave:Càncer de pulmó
Proliferació
Oncosferes
Comunicació cèl·lula-cèl·lula
Citoesquelet
Motilitat
Recurrència
Cèl·lules mare cancerígenes
Metàstasi
Cultius cel·lulars
Cáncer de pulmón
Proliferación
Oncosferas
Comunicación célula-célula
Citoesqueleto
Motilidad
Recurrencia
Células madre cancerígenas
Metástasis
Cultivos celulares
Lung cancer
Proliferation
Oncospheres
Cell-cell communication
Cytoskeleton
Motility
Recurrence
Carcinogenic stem cells
Metastasis
Cell cultures
61
616
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oai_identifier_str oai:recercat.cat:20.500.12328/3019
network_acronym_str ES
network_name_str España
repository_id_str
spelling Expression of oncogenic drivers in 3D cell culture depends on nuclear ATP synthesis by NUDT5Pickup, Katherine E.Pardow, FelicitasCarbonell-Caballero, JoséLioutas, AntoniosVillanueva-Cañas, José LuisWright, RoniBeato, MiguelCàncer de pulmóProliferacióOncosferesComunicació cèl·lula-cèl·lulaCitoesqueletMotilitatRecurrènciaCèl·lules mare cancerígenesMetàstasiCultius cel·lularsCáncer de pulmónProliferaciónOncosferasComunicación célula-célulaCitoesqueletoMotilidadRecurrenciaCélulas madre cancerígenasMetástasisCultivos celularesLung cancerProliferationOncospheresCell-cell communicationCytoskeletonMotilityRecurrenceCarcinogenic stem cellsMetastasisCell cultures61616The experimental work mentioned was supported by European Research Council (Project “4D Genome” 609989), the Ministerio de Economía y Competitividad (Project G62426937) and the Generalitat de Catalunya (Project AGAUR SGR 575).The growth of cancer cells as oncospheres in three-dimensional (3D) culture provides a robust cell model for understanding cancer progression, as well as for early drug discovery and validation. We have previously described a novel pathway in breast cancer cells, whereby ADP (Adenosine diphosphate)-ribose derived from hydrolysis of poly (ADP-Ribose) and pyrophosphate (PPi) are converted to ATP, catalysed by the enzyme NUDT5 (nucleotide diphosphate hydrolase type 5). Overexpression of the NUDT5 gene in breast and other cancer types is associated with poor prognosis, increased risk of recurrence and metastasis. In order to understand the role of NUDT5 in cancer cell growth, we performed phenotypic and global expression analysis in breast cancer cells grown as oncospheres. Comparison of two-dimensional (2D) versus 3D cancer cell cultures from different tissues of origin suggest that NUDT5 increases the aggressiveness of the disease via the modulation of several key driver genes, including ubiquitin specific peptidase 22 (USP22), RAB35B, focadhesin (FOCAD) and prostagladin E synthase (PTGES). NUDT5 functions as a master regulator of key oncogenic pathways and of genes involved in cell adhesion, cancer stem cell (CSC) maintenance and epithelial to mesenchyme transition (EMT). Inhibiting the enzymatic activities of NUDT5 prevents oncosphere formation and precludes the activation of cancer driver genes. These findings highlight NUDT5 as an upstream regulator of tumour drivers and may provide a biomarker for cancer stratification, as well as a novel target for drug discovery for combinatorial drug regimens for the treatment of aggressive cancer types and metastasis.info:eu-repo/semantics/publishedVersionMDPI2019info:eu-repo/semantics/article17http://hdl.handle.net/20.500.12328/3019https://dx.doi.org/10.3390/cancers11091337reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésCancers11;9This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:20.500.12328/30192026-05-29T05:05:01Z
dc.title.none.fl_str_mv Expression of oncogenic drivers in 3D cell culture depends on nuclear ATP synthesis by NUDT5
title Expression of oncogenic drivers in 3D cell culture depends on nuclear ATP synthesis by NUDT5
spellingShingle Expression of oncogenic drivers in 3D cell culture depends on nuclear ATP synthesis by NUDT5
Pickup, Katherine E.
Càncer de pulmó
Proliferació
Oncosferes
Comunicació cèl·lula-cèl·lula
Citoesquelet
Motilitat
Recurrència
Cèl·lules mare cancerígenes
Metàstasi
Cultius cel·lulars
Cáncer de pulmón
Proliferación
Oncosferas
Comunicación célula-célula
Citoesqueleto
Motilidad
Recurrencia
Células madre cancerígenas
Metástasis
Cultivos celulares
Lung cancer
Proliferation
Oncospheres
Cell-cell communication
Cytoskeleton
Motility
Recurrence
Carcinogenic stem cells
Metastasis
Cell cultures
61
616
title_short Expression of oncogenic drivers in 3D cell culture depends on nuclear ATP synthesis by NUDT5
title_full Expression of oncogenic drivers in 3D cell culture depends on nuclear ATP synthesis by NUDT5
title_fullStr Expression of oncogenic drivers in 3D cell culture depends on nuclear ATP synthesis by NUDT5
title_full_unstemmed Expression of oncogenic drivers in 3D cell culture depends on nuclear ATP synthesis by NUDT5
title_sort Expression of oncogenic drivers in 3D cell culture depends on nuclear ATP synthesis by NUDT5
dc.creator.none.fl_str_mv Pickup, Katherine E.
Pardow, Felicitas
Carbonell-Caballero, José
Lioutas, Antonios
Villanueva-Cañas, José Luis
Wright, Roni
Beato, Miguel
author Pickup, Katherine E.
author_facet Pickup, Katherine E.
Pardow, Felicitas
Carbonell-Caballero, José
Lioutas, Antonios
Villanueva-Cañas, José Luis
Wright, Roni
Beato, Miguel
author_role author
author2 Pardow, Felicitas
Carbonell-Caballero, José
Lioutas, Antonios
Villanueva-Cañas, José Luis
Wright, Roni
Beato, Miguel
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Càncer de pulmó
Proliferació
Oncosferes
Comunicació cèl·lula-cèl·lula
Citoesquelet
Motilitat
Recurrència
Cèl·lules mare cancerígenes
Metàstasi
Cultius cel·lulars
Cáncer de pulmón
Proliferación
Oncosferas
Comunicación célula-célula
Citoesqueleto
Motilidad
Recurrencia
Células madre cancerígenas
Metástasis
Cultivos celulares
Lung cancer
Proliferation
Oncospheres
Cell-cell communication
Cytoskeleton
Motility
Recurrence
Carcinogenic stem cells
Metastasis
Cell cultures
61
616
topic Càncer de pulmó
Proliferació
Oncosferes
Comunicació cèl·lula-cèl·lula
Citoesquelet
Motilitat
Recurrència
Cèl·lules mare cancerígenes
Metàstasi
Cultius cel·lulars
Cáncer de pulmón
Proliferación
Oncosferas
Comunicación célula-célula
Citoesqueleto
Motilidad
Recurrencia
Células madre cancerígenas
Metástasis
Cultivos celulares
Lung cancer
Proliferation
Oncospheres
Cell-cell communication
Cytoskeleton
Motility
Recurrence
Carcinogenic stem cells
Metastasis
Cell cultures
61
616
description The experimental work mentioned was supported by European Research Council (Project “4D Genome” 609989), the Ministerio de Economía y Competitividad (Project G62426937) and the Generalitat de Catalunya (Project AGAUR SGR 575).
publishDate 2019
dc.date.none.fl_str_mv 2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12328/3019
https://dx.doi.org/10.3390/cancers11091337
url http://hdl.handle.net/20.500.12328/3019
https://dx.doi.org/10.3390/cancers11091337
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Cancers
11;9
dc.rights.none.fl_str_mv https://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 17
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869403522579038208
score 15,812429

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