Knockdown of T-cell intracellular antigens triggers cell proliferation, invasion and tumour growth

TIA (T-cell intracellular antigen) proteins function as DNA/RNA trans-acting regulators to expand transcriptome and proteome diversity in mammals. In the present paper we report that the stable silencing of TIA1 and/or TIAR/TIAL1 (TIA1-related/like protein 1) expression in HeLa cells enhances cell p...

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Detalhes bibliográficos
Autores: Reyes, Raquel, Alcalde, José, Carrascoso, Isabel, Ludeña, María Dolores
Tipo de documento: artigo
Estado:Versión aceptada para publicación
Data de publicação:2011
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositório:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/343355
Acesso em linha:http://hdl.handle.net/10261/343355
Access Level:Acceso aberto
Palavra-chave:Gene expression control
T-cell intracellular antigen 1 (TIA1)
T-cell intracellular antigen 1-related protein (TIAR)
tumour progression
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spelling Knockdown of T-cell intracellular antigens triggers cell proliferation, invasion and tumour growthReyes, RaquelAlcalde, JoséCarrascoso, IsabelReyes, RaquelLudeña, María DoloresGene expression controlT-cell intracellular antigen 1 (TIA1)T-cell intracellular antigen 1-related protein (TIAR)tumour progressionTIA (T-cell intracellular antigen) proteins function as DNA/RNA trans-acting regulators to expand transcriptome and proteome diversity in mammals. In the present paper we report that the stable silencing of TIA1 and/or TIAR/TIAL1 (TIA1-related/like protein 1) expression in HeLa cells enhances cell proliferation, anchorage-dependent and -independent growth and invasion. HeLa cells lacking TIA1 and/or TIAR generate larger and faster-growing epithelial tumours with high rates of proliferation and angiogenesis in nude mice xenografts. Protein array analysis of a collection of human tumours shows that TIA1 and TIAR protein expression is down-regulated in a subset of epithelial tumours relative to normal tissues. Our results suggest a link between the epigenetic control exerted by TIA proteins and the transcriptional and post-transcriptional regulation of a subset of specific genes involved in tumour progression. Taken together, these results are consistent with a role for TIA proteins as growth/tumour-suppressor genes.This work was supported by the Fondo de Investigaciones Sanitarias-FEDER [grant number PI051605]; the Ministerio de Ciencia e Innovación-FEDER [grant number BFU2008–00354]; and the Agencia Estatal Consejo Superior de Investigaciones Científicas [grant number 200920I108]. The Centro de Biología Molecular ‘Severo Ochoa’ receives an institutional grant from the Fundación Ramón Areces, Spain.Peer reviewedBiochemical SocietyPortland PressEuropean CommissionMinisterio de Ciencia e Innovación (España)Consejo Superior de Investigaciones Científicas (España)Fundación Ramón ArecesConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202420242011info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionapplication/pdfhttp://hdl.handle.net/10261/343355reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MICINN//BFU2008-00354https://doi.org/10.1042/BJ20101030Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3433552026-05-22T06:33:51Z
dc.title.none.fl_str_mv Knockdown of T-cell intracellular antigens triggers cell proliferation, invasion and tumour growth
title Knockdown of T-cell intracellular antigens triggers cell proliferation, invasion and tumour growth
spellingShingle Knockdown of T-cell intracellular antigens triggers cell proliferation, invasion and tumour growth
Reyes, Raquel
Gene expression control
T-cell intracellular antigen 1 (TIA1)
T-cell intracellular antigen 1-related protein (TIAR)
tumour progression
title_short Knockdown of T-cell intracellular antigens triggers cell proliferation, invasion and tumour growth
title_full Knockdown of T-cell intracellular antigens triggers cell proliferation, invasion and tumour growth
title_fullStr Knockdown of T-cell intracellular antigens triggers cell proliferation, invasion and tumour growth
title_full_unstemmed Knockdown of T-cell intracellular antigens triggers cell proliferation, invasion and tumour growth
title_sort Knockdown of T-cell intracellular antigens triggers cell proliferation, invasion and tumour growth
dc.creator.none.fl_str_mv Reyes, Raquel
Alcalde, José
Carrascoso, Isabel
Reyes, Raquel
Ludeña, María Dolores
author Reyes, Raquel
author_facet Reyes, Raquel
Alcalde, José
Carrascoso, Isabel
Ludeña, María Dolores
author_role author
author2 Alcalde, José
Carrascoso, Isabel
Ludeña, María Dolores
author2_role author
author
author
dc.contributor.none.fl_str_mv European Commission
Ministerio de Ciencia e Innovación (España)
Consejo Superior de Investigaciones Científicas (España)
Fundación Ramón Areces
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Gene expression control
T-cell intracellular antigen 1 (TIA1)
T-cell intracellular antigen 1-related protein (TIAR)
tumour progression
topic Gene expression control
T-cell intracellular antigen 1 (TIA1)
T-cell intracellular antigen 1-related protein (TIAR)
tumour progression
description TIA (T-cell intracellular antigen) proteins function as DNA/RNA trans-acting regulators to expand transcriptome and proteome diversity in mammals. In the present paper we report that the stable silencing of TIA1 and/or TIAR/TIAL1 (TIA1-related/like protein 1) expression in HeLa cells enhances cell proliferation, anchorage-dependent and -independent growth and invasion. HeLa cells lacking TIA1 and/or TIAR generate larger and faster-growing epithelial tumours with high rates of proliferation and angiogenesis in nude mice xenografts. Protein array analysis of a collection of human tumours shows that TIA1 and TIAR protein expression is down-regulated in a subset of epithelial tumours relative to normal tissues. Our results suggest a link between the epigenetic control exerted by TIA proteins and the transcriptional and post-transcriptional regulation of a subset of specific genes involved in tumour progression. Taken together, these results are consistent with a role for TIA proteins as growth/tumour-suppressor genes.
publishDate 2011
dc.date.none.fl_str_mv 2011
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/343355
url http://hdl.handle.net/10261/343355
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MICINN//BFU2008-00354
https://doi.org/10.1042/BJ20101030
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Biochemical Society
Portland Press
publisher.none.fl_str_mv Biochemical Society
Portland Press
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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