Microcontroller-based sinusoidal voltage generation for electrical bio-impedance spectroscopy applications

A sinusoidal voltage wave generator is proposed based on the use of micro-processor digital signals with programmable duty-cycles, with application to real-time Electrical Cell-substrate Impedance Spectroscopy (ECIS) assays in cell cultures. The working principle relies on the time convolution of th...

Descripción completa

Detalles Bibliográficos
Autores: Castro, Juan A., Olmo, Alberto, Pérez, Pablo, Yúfera, Alberto
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2016
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/195084
Acceso en línea:http://hdl.handle.net/10261/195084
Access Level:acceso abierto
Palabra clave:Sinusoidal Voltage Generator
Electrical Cell-Substrate Impedance Spectroscopy (ECIS)
Bioimpedance
Microcontroller (µC)
Total Harmonic Distortion (THD)
id ES_104f5cc4bcd2d4ba6b0e07845712dd79
oai_identifier_str oai:digital.csic.es:10261/195084
network_acronym_str ES
network_name_str España
repository_id_str
spelling Microcontroller-based sinusoidal voltage generation for electrical bio-impedance spectroscopy applicationsCastro, Juan A.Olmo, AlbertoPérez, PabloYúfera, AlbertoSinusoidal Voltage GeneratorElectrical Cell-Substrate Impedance Spectroscopy (ECIS)BioimpedanceMicrocontroller (µC)Total Harmonic Distortion (THD)A sinusoidal voltage wave generator is proposed based on the use of micro-processor digital signals with programmable duty-cycles, with application to real-time Electrical Cell-substrate Impedance Spectroscopy (ECIS) assays in cell cultures. The working principle relies on the time convolution of the programmed microcontroller (μC) digital signals. The expected frequency is easily tuned on the bio-impedance spectroscopy range [100 Hz, 1 MHz] thanks to the μC clock frequency selection. This system has been simulated and tested on the 8 bits μC ArduinoTM Uno with ATmega328 version. Results obtained prove that only three digital signals are required to fit the general specification in ECIS experiments, below 1% THD accuracy, and show the appropriateness of the system for the real-time monitoring of this type of biological experiments.This work was supported in part by the Spanish founded Project: TEC 2013- 46242-C3-1-P: Integrated Microsystem for Cell Culture Assays, co-financed with FEDER.Peer reviewedScientific Research PublishingEuropean CommissionMinisterio de Economía y Competitividad (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201920192016info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/195084reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/TEC 2013-46242-C3-1-Phttp://dx.doi.org/10.4236/jcc.2016.417003Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1950842026-05-22T06:33:51Z
dc.title.none.fl_str_mv Microcontroller-based sinusoidal voltage generation for electrical bio-impedance spectroscopy applications
title Microcontroller-based sinusoidal voltage generation for electrical bio-impedance spectroscopy applications
spellingShingle Microcontroller-based sinusoidal voltage generation for electrical bio-impedance spectroscopy applications
Castro, Juan A.
Sinusoidal Voltage Generator
Electrical Cell-Substrate Impedance Spectroscopy (ECIS)
Bioimpedance
Microcontroller (µC)
Total Harmonic Distortion (THD)
title_short Microcontroller-based sinusoidal voltage generation for electrical bio-impedance spectroscopy applications
title_full Microcontroller-based sinusoidal voltage generation for electrical bio-impedance spectroscopy applications
title_fullStr Microcontroller-based sinusoidal voltage generation for electrical bio-impedance spectroscopy applications
title_full_unstemmed Microcontroller-based sinusoidal voltage generation for electrical bio-impedance spectroscopy applications
title_sort Microcontroller-based sinusoidal voltage generation for electrical bio-impedance spectroscopy applications
dc.creator.none.fl_str_mv Castro, Juan A.
Olmo, Alberto
Pérez, Pablo
Yúfera, Alberto
author Castro, Juan A.
author_facet Castro, Juan A.
Olmo, Alberto
Pérez, Pablo
Yúfera, Alberto
author_role author
author2 Olmo, Alberto
Pérez, Pablo
Yúfera, Alberto
author2_role author
author
author
dc.contributor.none.fl_str_mv European Commission
Ministerio de Economía y Competitividad (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Sinusoidal Voltage Generator
Electrical Cell-Substrate Impedance Spectroscopy (ECIS)
Bioimpedance
Microcontroller (µC)
Total Harmonic Distortion (THD)
topic Sinusoidal Voltage Generator
Electrical Cell-Substrate Impedance Spectroscopy (ECIS)
Bioimpedance
Microcontroller (µC)
Total Harmonic Distortion (THD)
description A sinusoidal voltage wave generator is proposed based on the use of micro-processor digital signals with programmable duty-cycles, with application to real-time Electrical Cell-substrate Impedance Spectroscopy (ECIS) assays in cell cultures. The working principle relies on the time convolution of the programmed microcontroller (μC) digital signals. The expected frequency is easily tuned on the bio-impedance spectroscopy range [100 Hz, 1 MHz] thanks to the μC clock frequency selection. This system has been simulated and tested on the 8 bits μC ArduinoTM Uno with ATmega328 version. Results obtained prove that only three digital signals are required to fit the general specification in ECIS experiments, below 1% THD accuracy, and show the appropriateness of the system for the real-time monitoring of this type of biological experiments.
publishDate 2016
dc.date.none.fl_str_mv 2016
2019
2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/195084
url http://hdl.handle.net/10261/195084
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/TEC 2013-46242-C3-1-P
http://dx.doi.org/10.4236/jcc.2016.417003
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Scientific Research Publishing
publisher.none.fl_str_mv Scientific Research Publishing
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869403502569062400
score 15,812455

Similares en LA Referencia