Neuronal induction and bioenergetics characterization of human forearm adipose stem cells from Parkinson’s disease patients and healthy controls

Parkinson's disease; Stem regenerative medicine; Bioenergetics

Detalles Bibliográficos
Autores: González-Casacuberta, Ingrid, Pont-Sunyer, Claustre, Tobías, Ester, Cantó Santos, Judith, Valls-Roca, Laura, Vilas, Dolores
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:11351/7357
Acceso en línea:https://hdl.handle.net/11351/7357
Access Level:acceso abierto
Palabra clave:Parkinson, Malaltia de
Medicina regenerativa
Bioenergètica
DISEASES::Nervous System Diseases::Central Nervous System Diseases::Brain Diseases::Basal Ganglia Diseases::Parkinsonian Disorders::Parkinson Disease
DISCIPLINES AND OCCUPATIONS::Health Occupations::Medicine::Regenerative Medicine
PHENOMENA AND PROCESSES::Metabolism::Energy Metabolism
ENFERMEDADES::enfermedades del sistema nervioso::enfermedades del sistema nervioso central::enfermedades cerebrales::enfermedades de los ganglios basales::trastornos parkinsonianos::enfermedad de Parkinson
DISCIPLINAS Y OCUPACIONES::profesiones sanitarias::medicina::medicina regenerativa
FENÓMENOS Y PROCESOS::metabolismo::metabolismo energético
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spelling Neuronal induction and bioenergetics characterization of human forearm adipose stem cells from Parkinson’s disease patients and healthy controlsGonzález-Casacuberta, IngridPont-Sunyer, ClaustreTobías, EsterCantó Santos, JudithValls-Roca, LauraVilas, DoloresParkinson, Malaltia deMedicina regenerativaBioenergèticaDISEASES::Nervous System Diseases::Central Nervous System Diseases::Brain Diseases::Basal Ganglia Diseases::Parkinsonian Disorders::Parkinson DiseaseDISCIPLINES AND OCCUPATIONS::Health Occupations::Medicine::Regenerative MedicinePHENOMENA AND PROCESSES::Metabolism::Energy MetabolismENFERMEDADES::enfermedades del sistema nervioso::enfermedades del sistema nervioso central::enfermedades cerebrales::enfermedades de los ganglios basales::trastornos parkinsonianos::enfermedad de ParkinsonDISCIPLINAS Y OCUPACIONES::profesiones sanitarias::medicina::medicina regenerativaFENÓMENOS Y PROCESOS::metabolismo::metabolismo energéticoParkinson's disease; Stem regenerative medicine; BioenergeticsEnfermedad de Parkinson; Medicina regenerativa del tallo; BioenergéticaMalaltia de Parkinson; Medicina regenerativa de tija; BioenergèticaNeurodegenerative diseases, such as Parkinson's disease, are heterogeneous disorders with a multifactorial nature involving impaired bioenergetics. Stem-regenerative medicine and bioenergetics have been proposed as promising therapeutic targets in the neurologic field. The rationale of the present study was to assess the potential of human-derived adipose stem cells (hASCs) to transdifferentiate into neuronal-like cells (NhASCs and neurospheres) and explore the hASC bioenergetic profile. hASC neuronal transdifferentiation was performed through neurobasal media and differentiation factor exposure. High resolution respirometry was assessed. Increased MAP-2 neuronal marker protein expression upon neuronal induction (p<0.05 undifferentiated hASCs vs. 28-36 days of differentiation) and increased bIII-tubulin neuronal marker protein expression upon neuronal induction (p<0.05 undifferentiated hASCs vs. 6-28-36 days of differentiation) were found. The bioenergetic profile was detectable through high-resolution respirometry approaches in hASCs but did not lead to differential oxidative capacity rates in healthy or clinically diagnosed PD-hASCs. We confirmed the capability of transdifferentiation to the neuronal-like profile of hASCs derived from the forearms of human subjects and characterized the bioenergetic profile. Suboptimal maximal respiratory capacity trends in PD were found. Neuronal induction leading to positive neuronal protein expression markers is a relevant issue that encourages the suitability of NhASC models in neurodegeneration.Public Library of Science[González-Casacuberta I, Tobías E, Cantó-Santos J, Valls-Roca L] Cellex-Institut d’Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Faculty of Medicine and Health Science, University of Barcelona, Spain. Internal Medicine Department, Hospital Clínic of Barcelona, Barcelona, Spain. Biomedical Research Networking Center on Rare Diseases (CIBERER), Madrid, Spain. [Vilas D] Neurodegenerative Diseases Unit, Neurology Service, University Hospital Germans Trias i Pujol, Badalona, Catalonia, Spain. [Pont-Sunyer C] Unitat de Neurologia, Hospital General de Granollers, Granollers, Spain. Universitat Internacional de Catalunya, Barcelona, SpainHospital General de Granollers202220222022info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/11351/7357Scientiareponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésPLoS ONE;17(3)https://doi.org/10.1371/journal.pone.0265256Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:11351/73572026-05-29T05:05:01Z
dc.title.none.fl_str_mv Neuronal induction and bioenergetics characterization of human forearm adipose stem cells from Parkinson’s disease patients and healthy controls
title Neuronal induction and bioenergetics characterization of human forearm adipose stem cells from Parkinson’s disease patients and healthy controls
spellingShingle Neuronal induction and bioenergetics characterization of human forearm adipose stem cells from Parkinson’s disease patients and healthy controls
González-Casacuberta, Ingrid
Parkinson, Malaltia de
Medicina regenerativa
Bioenergètica
DISEASES::Nervous System Diseases::Central Nervous System Diseases::Brain Diseases::Basal Ganglia Diseases::Parkinsonian Disorders::Parkinson Disease
DISCIPLINES AND OCCUPATIONS::Health Occupations::Medicine::Regenerative Medicine
PHENOMENA AND PROCESSES::Metabolism::Energy Metabolism
ENFERMEDADES::enfermedades del sistema nervioso::enfermedades del sistema nervioso central::enfermedades cerebrales::enfermedades de los ganglios basales::trastornos parkinsonianos::enfermedad de Parkinson
DISCIPLINAS Y OCUPACIONES::profesiones sanitarias::medicina::medicina regenerativa
FENÓMENOS Y PROCESOS::metabolismo::metabolismo energético
title_short Neuronal induction and bioenergetics characterization of human forearm adipose stem cells from Parkinson’s disease patients and healthy controls
title_full Neuronal induction and bioenergetics characterization of human forearm adipose stem cells from Parkinson’s disease patients and healthy controls
title_fullStr Neuronal induction and bioenergetics characterization of human forearm adipose stem cells from Parkinson’s disease patients and healthy controls
title_full_unstemmed Neuronal induction and bioenergetics characterization of human forearm adipose stem cells from Parkinson’s disease patients and healthy controls
title_sort Neuronal induction and bioenergetics characterization of human forearm adipose stem cells from Parkinson’s disease patients and healthy controls
dc.creator.none.fl_str_mv González-Casacuberta, Ingrid
Pont-Sunyer, Claustre
Tobías, Ester
Cantó Santos, Judith
Valls-Roca, Laura
Vilas, Dolores
author González-Casacuberta, Ingrid
author_facet González-Casacuberta, Ingrid
Pont-Sunyer, Claustre
Tobías, Ester
Cantó Santos, Judith
Valls-Roca, Laura
Vilas, Dolores
author_role author
author2 Pont-Sunyer, Claustre
Tobías, Ester
Cantó Santos, Judith
Valls-Roca, Laura
Vilas, Dolores
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv [González-Casacuberta I, Tobías E, Cantó-Santos J, Valls-Roca L] Cellex-Institut d’Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Faculty of Medicine and Health Science, University of Barcelona, Spain. Internal Medicine Department, Hospital Clínic of Barcelona, Barcelona, Spain. Biomedical Research Networking Center on Rare Diseases (CIBERER), Madrid, Spain. [Vilas D] Neurodegenerative Diseases Unit, Neurology Service, University Hospital Germans Trias i Pujol, Badalona, Catalonia, Spain. [Pont-Sunyer C] Unitat de Neurologia, Hospital General de Granollers, Granollers, Spain. Universitat Internacional de Catalunya, Barcelona, Spain
Hospital General de Granollers
dc.subject.none.fl_str_mv Parkinson, Malaltia de
Medicina regenerativa
Bioenergètica
DISEASES::Nervous System Diseases::Central Nervous System Diseases::Brain Diseases::Basal Ganglia Diseases::Parkinsonian Disorders::Parkinson Disease
DISCIPLINES AND OCCUPATIONS::Health Occupations::Medicine::Regenerative Medicine
PHENOMENA AND PROCESSES::Metabolism::Energy Metabolism
ENFERMEDADES::enfermedades del sistema nervioso::enfermedades del sistema nervioso central::enfermedades cerebrales::enfermedades de los ganglios basales::trastornos parkinsonianos::enfermedad de Parkinson
DISCIPLINAS Y OCUPACIONES::profesiones sanitarias::medicina::medicina regenerativa
FENÓMENOS Y PROCESOS::metabolismo::metabolismo energético
topic Parkinson, Malaltia de
Medicina regenerativa
Bioenergètica
DISEASES::Nervous System Diseases::Central Nervous System Diseases::Brain Diseases::Basal Ganglia Diseases::Parkinsonian Disorders::Parkinson Disease
DISCIPLINES AND OCCUPATIONS::Health Occupations::Medicine::Regenerative Medicine
PHENOMENA AND PROCESSES::Metabolism::Energy Metabolism
ENFERMEDADES::enfermedades del sistema nervioso::enfermedades del sistema nervioso central::enfermedades cerebrales::enfermedades de los ganglios basales::trastornos parkinsonianos::enfermedad de Parkinson
DISCIPLINAS Y OCUPACIONES::profesiones sanitarias::medicina::medicina regenerativa
FENÓMENOS Y PROCESOS::metabolismo::metabolismo energético
description Parkinson's disease; Stem regenerative medicine; Bioenergetics
publishDate 2022
dc.date.none.fl_str_mv 2022
2022
2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/11351/7357
url https://hdl.handle.net/11351/7357
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv PLoS ONE;17(3)
https://doi.org/10.1371/journal.pone.0265256
dc.rights.none.fl_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv Scientia
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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