An inversion on chromosome 8 is associated with individual resistance to Bonamia ostreae in the European flat oyster

The European flat oyster (Ostrea edulis) has experienced severe population declines across Europe, largely due to infection by Bonamia ostreae. Previous studies identified a chromosomal inversion on chromosome 8 (C8) associated with resistance to this parasite at population level, but its associatio...

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Detalhes bibliográficos
Autores: Martínez Sambade, Inés, Hlordzi, Vivian, Gómez Pardo, María Belén, Vera Rodríguez, Manuel, Culloty, Sarah C., Lynch, Sharon A., Martínez Portela, Paulino
Tipo de documento: artigo
Data de publicação:2026
País:España
Recursos:Universidad de Santiago de Compostela (USC)
Repositório:Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela
Idioma:inglês
OAI Identifier:oai:dnet:minerva_____::e241e7221ca81a243cfb044fcb0a5741
Acesso em linha:https://hdl.handle.net/10347/47596
Access Level:Acceso aberto
Palavra-chave:Ostrea edulis
Bonamiosis
Structural variation
Additive model
Marker-assisted selection
2401 Biología animal (zoología)
2409 Genética
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spelling An inversion on chromosome 8 is associated with individual resistance to Bonamia ostreae in the European flat oysterMartínez Sambade, InésHlordzi, VivianGómez Pardo, María BelénVera Rodríguez, ManuelCulloty, Sarah C.Lynch, Sharon A.Martínez Portela, PaulinoOstrea edulisBonamiosisStructural variationAdditive modelMarker-assisted selection2401 Biología animal (zoología)2409 GenéticaThe European flat oyster (Ostrea edulis) has experienced severe population declines across Europe, largely due to infection by Bonamia ostreae. Previous studies identified a chromosomal inversion on chromosome 8 (C8) associated with resistance to this parasite at population level, but its association at individual level has not yet been demonstrated. Here, we investigated three Irish shellfish beds (Lough Swilly, Galway, and Rossmore) with contrasting bonamiosis histories combining smear/microscopy diagnosis and qualitative PCR detection to evaluate parasite presence/load in heart and gill on a sample of 436 oysters. A medium-density SNP array was used for genotyping the three large flat oyster inversions to check for karyotype association both using binary (uninfected vs infected) and semi-quantitative (infection level) traits. We found a strong association at individual level between the C8 inversion and B. ostreae infection in heart tissue but not in gill, suggesting a resistance mechanism hampering the entrance of the parasite to infect haemocytes. An additive model of association was the best fitted to data and an overdominant model was fully discarded. No association was detected for the two other large inversions of flat oyster on C2 and C5. Based on these findings, a cost-effective five-SNP MassARRAY panel was developed to genotype the C8 inversion efficiently using as reference the whole SNP panel. Our results confirm that the C8 inversion holds a key mechanism of resistance to B. ostreae and provide a practical tool for marker-assisted selection in O. edulis to enhance resistance in restoration and breeding programs.ElsevierUniversidade de Santiago de Compostela. Departamento de Zooloxía e Antropoloxía Física20262026-04-0720262026-04-07journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10347/47596reponame:Minerva. Repositorio Institucional de la Universidad de Santiago de Compostelainstname:Universidad de Santiago de Compostela (USC)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2© 2026 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY licensehttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:dnet:minerva_____::e241e7221ca81a243cfb044fcb0a57412026-06-15T12:47:27Z
dc.title.none.fl_str_mv An inversion on chromosome 8 is associated with individual resistance to Bonamia ostreae in the European flat oyster
title An inversion on chromosome 8 is associated with individual resistance to Bonamia ostreae in the European flat oyster
spellingShingle An inversion on chromosome 8 is associated with individual resistance to Bonamia ostreae in the European flat oyster
Martínez Sambade, Inés
Ostrea edulis
Bonamiosis
Structural variation
Additive model
Marker-assisted selection
2401 Biología animal (zoología)
2409 Genética
title_short An inversion on chromosome 8 is associated with individual resistance to Bonamia ostreae in the European flat oyster
title_full An inversion on chromosome 8 is associated with individual resistance to Bonamia ostreae in the European flat oyster
title_fullStr An inversion on chromosome 8 is associated with individual resistance to Bonamia ostreae in the European flat oyster
title_full_unstemmed An inversion on chromosome 8 is associated with individual resistance to Bonamia ostreae in the European flat oyster
title_sort An inversion on chromosome 8 is associated with individual resistance to Bonamia ostreae in the European flat oyster
dc.creator.none.fl_str_mv Martínez Sambade, Inés
Hlordzi, Vivian
Gómez Pardo, María Belén
Vera Rodríguez, Manuel
Culloty, Sarah C.
Lynch, Sharon A.
Martínez Portela, Paulino
author Martínez Sambade, Inés
author_facet Martínez Sambade, Inés
Hlordzi, Vivian
Gómez Pardo, María Belén
Vera Rodríguez, Manuel
Culloty, Sarah C.
Lynch, Sharon A.
Martínez Portela, Paulino
author_role author
author2 Hlordzi, Vivian
Gómez Pardo, María Belén
Vera Rodríguez, Manuel
Culloty, Sarah C.
Lynch, Sharon A.
Martínez Portela, Paulino
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade de Santiago de Compostela. Departamento de Zooloxía e Antropoloxía Física
dc.subject.none.fl_str_mv Ostrea edulis
Bonamiosis
Structural variation
Additive model
Marker-assisted selection
2401 Biología animal (zoología)
2409 Genética
topic Ostrea edulis
Bonamiosis
Structural variation
Additive model
Marker-assisted selection
2401 Biología animal (zoología)
2409 Genética
description The European flat oyster (Ostrea edulis) has experienced severe population declines across Europe, largely due to infection by Bonamia ostreae. Previous studies identified a chromosomal inversion on chromosome 8 (C8) associated with resistance to this parasite at population level, but its association at individual level has not yet been demonstrated. Here, we investigated three Irish shellfish beds (Lough Swilly, Galway, and Rossmore) with contrasting bonamiosis histories combining smear/microscopy diagnosis and qualitative PCR detection to evaluate parasite presence/load in heart and gill on a sample of 436 oysters. A medium-density SNP array was used for genotyping the three large flat oyster inversions to check for karyotype association both using binary (uninfected vs infected) and semi-quantitative (infection level) traits. We found a strong association at individual level between the C8 inversion and B. ostreae infection in heart tissue but not in gill, suggesting a resistance mechanism hampering the entrance of the parasite to infect haemocytes. An additive model of association was the best fitted to data and an overdominant model was fully discarded. No association was detected for the two other large inversions of flat oyster on C2 and C5. Based on these findings, a cost-effective five-SNP MassARRAY panel was developed to genotype the C8 inversion efficiently using as reference the whole SNP panel. Our results confirm that the C8 inversion holds a key mechanism of resistance to B. ostreae and provide a practical tool for marker-assisted selection in O. edulis to enhance resistance in restoration and breeding programs.
publishDate 2026
dc.date.none.fl_str_mv 2026
2026-04-07
2026
2026-04-07
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/10347/47596
url https://hdl.handle.net/10347/47596
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela
instname:Universidad de Santiago de Compostela (USC)
instname_str Universidad de Santiago de Compostela (USC)
reponame_str Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela
collection Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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