cAMP-responsive element-binding protein (CREB) and cAMP co-regulate activator protein 1 (AP1)-dependent regeneration-associated gene expression and neurite growth

To regenerate damaged axons, neurons must express a cassette of regeneration-associated genes (RAGs) that increases intrinsic growth capacity and confers resistance to extrinsic inhibitory cues. Here we show that dibutyrl-cAMP or forskolin combined with constitutive-active CREB are superior to eithe...

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Detalles Bibliográficos
Autores: Ma, Thong C., Barco, Ángel, Ratan, Rajiv R., Willis, Dianna E.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2014
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/288936
Acceso en línea:http://hdl.handle.net/10261/288936
Access Level:acceso abierto
Palabra clave:AP1 transcription factor (AP-1)
cAMP response element-binding protein (CREB)
Cyclic AMP (cAMP)
Regeneration-associated gene
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spelling cAMP-responsive element-binding protein (CREB) and cAMP co-regulate activator protein 1 (AP1)-dependent regeneration-associated gene expression and neurite growthMa, Thong C.Barco, ÁngelRatan, Rajiv R.Willis, Dianna E.AP1 transcription factor (AP-1)cAMP response element-binding protein (CREB)Cyclic AMP (cAMP)Regeneration-associated geneTo regenerate damaged axons, neurons must express a cassette of regeneration-associated genes (RAGs) that increases intrinsic growth capacity and confers resistance to extrinsic inhibitory cues. Here we show that dibutyrl-cAMP or forskolin combined with constitutive-active CREB are superior to either agent alone in driving neurite growth on permissive and inhibitory substrates. Of the RAGs examined, only arginase 1 (Arg1) expression correlated with the increased neurite growth induced by the cAMP/CREB combination, both of which were AP1-dependent. This suggests that cAMP-induced AP1 activity is necessary and interacts with CREB to drive expression of RAGs relevant for regeneration and demonstrates that combining a small molecule (cAMP) with an activated transcription factor (CREB) stimulates the gene expression necessary to enhance axonal regeneration.This work was supported, in whole or in part, by National Institutes of Health Grant NR010797 (to D. W.). This work was also supported by grants from the Dr. Miriam and Sheldon G. Adelson Foundation (to R. R.), The Center for Research Excellence in Spinal Cord Injury (Grant DOHC019772; to R. R.), and the Burke Medical Research Institute Foundation (to D. W.). The Barco laboratory is supported by Grants SAF2011-22855 from the Spanish Ministry of Science and Innovation and Prometeo/2012/005 from the Generalitat ValencianaPeer reviewedElsevierNational Institutes of Health (US)Adelson Medical Research FoundationGeneralitat ValencianaMinisterio de Ciencia e Innovación (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202320232014info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/288936reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MICINN//SAF2011-22855Journal of Biological Chemistryhttps://doi.org/10.1074/jbc.M114.582460Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2889362026-05-22T06:33:51Z
dc.title.none.fl_str_mv cAMP-responsive element-binding protein (CREB) and cAMP co-regulate activator protein 1 (AP1)-dependent regeneration-associated gene expression and neurite growth
title cAMP-responsive element-binding protein (CREB) and cAMP co-regulate activator protein 1 (AP1)-dependent regeneration-associated gene expression and neurite growth
spellingShingle cAMP-responsive element-binding protein (CREB) and cAMP co-regulate activator protein 1 (AP1)-dependent regeneration-associated gene expression and neurite growth
Ma, Thong C.
AP1 transcription factor (AP-1)
cAMP response element-binding protein (CREB)
Cyclic AMP (cAMP)
Regeneration-associated gene
title_short cAMP-responsive element-binding protein (CREB) and cAMP co-regulate activator protein 1 (AP1)-dependent regeneration-associated gene expression and neurite growth
title_full cAMP-responsive element-binding protein (CREB) and cAMP co-regulate activator protein 1 (AP1)-dependent regeneration-associated gene expression and neurite growth
title_fullStr cAMP-responsive element-binding protein (CREB) and cAMP co-regulate activator protein 1 (AP1)-dependent regeneration-associated gene expression and neurite growth
title_full_unstemmed cAMP-responsive element-binding protein (CREB) and cAMP co-regulate activator protein 1 (AP1)-dependent regeneration-associated gene expression and neurite growth
title_sort cAMP-responsive element-binding protein (CREB) and cAMP co-regulate activator protein 1 (AP1)-dependent regeneration-associated gene expression and neurite growth
dc.creator.none.fl_str_mv Ma, Thong C.
Barco, Ángel
Ratan, Rajiv R.
Willis, Dianna E.
author Ma, Thong C.
author_facet Ma, Thong C.
Barco, Ángel
Ratan, Rajiv R.
Willis, Dianna E.
author_role author
author2 Barco, Ángel
Ratan, Rajiv R.
Willis, Dianna E.
author2_role author
author
author
dc.contributor.none.fl_str_mv National Institutes of Health (US)
Adelson Medical Research Foundation
Generalitat Valenciana
Ministerio de Ciencia e Innovación (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv AP1 transcription factor (AP-1)
cAMP response element-binding protein (CREB)
Cyclic AMP (cAMP)
Regeneration-associated gene
topic AP1 transcription factor (AP-1)
cAMP response element-binding protein (CREB)
Cyclic AMP (cAMP)
Regeneration-associated gene
description To regenerate damaged axons, neurons must express a cassette of regeneration-associated genes (RAGs) that increases intrinsic growth capacity and confers resistance to extrinsic inhibitory cues. Here we show that dibutyrl-cAMP or forskolin combined with constitutive-active CREB are superior to either agent alone in driving neurite growth on permissive and inhibitory substrates. Of the RAGs examined, only arginase 1 (Arg1) expression correlated with the increased neurite growth induced by the cAMP/CREB combination, both of which were AP1-dependent. This suggests that cAMP-induced AP1 activity is necessary and interacts with CREB to drive expression of RAGs relevant for regeneration and demonstrates that combining a small molecule (cAMP) with an activated transcription factor (CREB) stimulates the gene expression necessary to enhance axonal regeneration.
publishDate 2014
dc.date.none.fl_str_mv 2014
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/288936
url http://hdl.handle.net/10261/288936
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MICINN//SAF2011-22855
Journal of Biological Chemistry
https://doi.org/10.1074/jbc.M114.582460
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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