Induced oxidative eustress stimulates glucose uptake in C2C12 myotubes and skeletal muscle fibres

Oxidative eustress -moderate intracellular levels of Reactive Oxygen and Nitrogen Species (RONS)- plays an important role modulating redox signalling processes in the cell. Glucose is vital in cells since this molecule provides the energy to maintain essential cellular functions. The reviewed litera...

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Autores: Martín-Prieto, Eva, Catalano-Iniesta, Leonardo A., Fernández-Puente, Escarlata, Palomero Labajos, Jesús
Tipo de documento: artigo
Estado:Versão publicada
Data de publicação:2025
País:España
Recursos:Universidad de Salamanca (USAL)
Repositório:GREDOS. Repositorio Institucional de la Universidad de Salamanca
OAI Identifier:oai:gredos.usal.es:10366/166159
Acesso em linha:http://hdl.handle.net/10366/166159
Access Level:Acesso embargado
Palavra-chave:ROS
RNS
RONS
Glucose uptake
Insulin resistance
3209 Farmacología
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spelling Induced oxidative eustress stimulates glucose uptake in C2C12 myotubes and skeletal muscle fibresMartín-Prieto, EvaCatalano-Iniesta, Leonardo A.Fernández-Puente, EscarlataPalomero Labajos, JesúsROSRNSRONSGlucose uptakeInsulin resistance3209 FarmacologíaOxidative eustress -moderate intracellular levels of Reactive Oxygen and Nitrogen Species (RONS)- plays an important role modulating redox signalling processes in the cell. Glucose is vital in cells since this molecule provides the energy to maintain essential cellular functions. The reviewed literature supports that the balance and concentration of RONS affect glucose uptake in skeletal muscle. AMPK and AKT are proteins involved in signaling pathways that regulate glucose uptake. Their phosphorylation evokes the translocation of glucose transporter GLUT4 from cytosolic vesicles to the plasma membrane, enabling glucose internalization into the cells. Using methodology previously developed and validated in our laboratory – quantitative fluorescence microscopy, immunocytochemistry, and protein immunodetection –, we have explored how RONS homeostasis modulates signaling pathways. These pathways, mediated by AMPK and AKT phosphorylation, promotes GLUT4 translocation, which facilitates glucose uptake and internalization in skeletal muscle cells (myotubes and fibres). The results showed that a pro-oxidative intracellular redox environment evoked by RONS, potentially linked with oxidative eustress, activates signalling pathways (P-AMPK or P-AKT), GLUT-4 translocation from cytosol to plasma membrane and, consequently, an increase in glucose uptake. In conclusion, the RONS-induced phosphorylation of AMPK and AKT proteins improves glucose uptake in C2C12 myotubes and single skeletal muscle fibres. In perspective, our findings would lead a potential therapeutic advance that overcome the impairment to internalize glucose in the cell -insulin resistance- onset in disorders such as diabetes and obesity, and in ageing.Elsevierinfo202520252025info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10366/166159reponame:GREDOS. Repositorio Institucional de la Universidad de Salamancainstname:Universidad de Salamanca (USAL)InglésAttribution-NonCommercial-NoDerivatives 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/embargoedAccessoai:gredos.usal.es:10366/1661592026-06-07T06:28:51Z
dc.title.none.fl_str_mv Induced oxidative eustress stimulates glucose uptake in C2C12 myotubes and skeletal muscle fibres
title Induced oxidative eustress stimulates glucose uptake in C2C12 myotubes and skeletal muscle fibres
spellingShingle Induced oxidative eustress stimulates glucose uptake in C2C12 myotubes and skeletal muscle fibres
Martín-Prieto, Eva
ROS
RNS
RONS
Glucose uptake
Insulin resistance
3209 Farmacología
title_short Induced oxidative eustress stimulates glucose uptake in C2C12 myotubes and skeletal muscle fibres
title_full Induced oxidative eustress stimulates glucose uptake in C2C12 myotubes and skeletal muscle fibres
title_fullStr Induced oxidative eustress stimulates glucose uptake in C2C12 myotubes and skeletal muscle fibres
title_full_unstemmed Induced oxidative eustress stimulates glucose uptake in C2C12 myotubes and skeletal muscle fibres
title_sort Induced oxidative eustress stimulates glucose uptake in C2C12 myotubes and skeletal muscle fibres
dc.creator.none.fl_str_mv Martín-Prieto, Eva
Catalano-Iniesta, Leonardo A.
Fernández-Puente, Escarlata
Palomero Labajos, Jesús
author Martín-Prieto, Eva
author_facet Martín-Prieto, Eva
Catalano-Iniesta, Leonardo A.
Fernández-Puente, Escarlata
Palomero Labajos, Jesús
author_role author
author2 Catalano-Iniesta, Leonardo A.
Fernández-Puente, Escarlata
Palomero Labajos, Jesús
author2_role author
author
author
dc.subject.none.fl_str_mv ROS
RNS
RONS
Glucose uptake
Insulin resistance
3209 Farmacología
topic ROS
RNS
RONS
Glucose uptake
Insulin resistance
3209 Farmacología
description Oxidative eustress -moderate intracellular levels of Reactive Oxygen and Nitrogen Species (RONS)- plays an important role modulating redox signalling processes in the cell. Glucose is vital in cells since this molecule provides the energy to maintain essential cellular functions. The reviewed literature supports that the balance and concentration of RONS affect glucose uptake in skeletal muscle. AMPK and AKT are proteins involved in signaling pathways that regulate glucose uptake. Their phosphorylation evokes the translocation of glucose transporter GLUT4 from cytosolic vesicles to the plasma membrane, enabling glucose internalization into the cells. Using methodology previously developed and validated in our laboratory – quantitative fluorescence microscopy, immunocytochemistry, and protein immunodetection –, we have explored how RONS homeostasis modulates signaling pathways. These pathways, mediated by AMPK and AKT phosphorylation, promotes GLUT4 translocation, which facilitates glucose uptake and internalization in skeletal muscle cells (myotubes and fibres). The results showed that a pro-oxidative intracellular redox environment evoked by RONS, potentially linked with oxidative eustress, activates signalling pathways (P-AMPK or P-AKT), GLUT-4 translocation from cytosol to plasma membrane and, consequently, an increase in glucose uptake. In conclusion, the RONS-induced phosphorylation of AMPK and AKT proteins improves glucose uptake in C2C12 myotubes and single skeletal muscle fibres. In perspective, our findings would lead a potential therapeutic advance that overcome the impairment to internalize glucose in the cell -insulin resistance- onset in disorders such as diabetes and obesity, and in ageing.
publishDate 2025
dc.date.none.fl_str_mv 2025
2025
2025
info
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10366/166159
url http://hdl.handle.net/10366/166159
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/embargoedAccess
rights_invalid_str_mv Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv embargoedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:GREDOS. Repositorio Institucional de la Universidad de Salamanca
instname:Universidad de Salamanca (USAL)
instname_str Universidad de Salamanca (USAL)
reponame_str GREDOS. Repositorio Institucional de la Universidad de Salamanca
collection GREDOS. Repositorio Institucional de la Universidad de Salamanca
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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