RONS-mediated redox signaling in skeletal muscle: Impact on glucose uptake through AMPK and AKT pathways
[EN]This study investigates how moderate levels of reactive oxygen and nitrogen species (RONS), known as oxidative eustress, affect glucose uptake in skeletal muscle. The findings show that a pro-oxidative intracellular environment increases the phosphorylation of AMPK and AKT proteins, promoting th...
| Autores: | , , |
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| Tipo de documento: | artigo |
| Estado: | Versão publicada |
| Data de publicação: | 2024 |
| País: | España |
| Recursos: | Universidad de Salamanca (USAL) |
| Repositório: | GREDOS. Repositorio Institucional de la Universidad de Salamanca |
| OAI Identifier: | oai:gredos.usal.es:10366/166163 |
| Acesso em linha: | http://hdl.handle.net/10366/166163 |
| Access Level: | Acceso aberto |
| Palavra-chave: | RONS AMPK Skeletal muscle Glucose 3209 Farmacología |
| Resumo: | [EN]This study investigates how moderate levels of reactive oxygen and nitrogen species (RONS), known as oxidative eustress, affect glucose uptake in skeletal muscle. The findings show that a pro-oxidative intracellular environment increases the phosphorylation of AMPK and AKT proteins, promoting the translocation of the GLUT4 transporter to the plasma membrane and enhancing glucose uptake. Using a fluorescent glucose analogue (6-NBDG) along with fluorescence microscopy, western blot, and immunocytochemistry, the researchers demonstrate that RONS-induced activation of AMPK and AKT signaling pathways can facilitate glucose uptake independently of insulin. These results highlight a redox-mediated alternative pathway for glucose metabolism in muscle cells. |
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