Transplantation of mesenchymal stem cells exerts a greater long-term effect than bone marrow mononuclear cells in a chronic myocardial infarction model in rat

The aim of this study is to assess the long-term effect of mesenchymal stem cells (MSC) transplantation in a rat model of chronic myocardial infarction (MI) in comparison with the effect of bone marrow mononuclear cells (BM-MNC) transplant. Five weeks after induction of MI, rats were allocated to re...

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Autores: Mazo-Vega, M.M. (Manuel María)|||/items/c871e8d9-ee5d-487b-97ef-7302feb8a02e, Gavira-Gómez, J.J. (Juan José)|||/items/a253ead5-4ef7-4b80-9aab-43b47605270e, Abizanda-Sarasa, G. (Gloria)|||/items/1e76a0e3-32d0-424e-b58a-d9ddf7a82ef7, Moreno-Parado, C. (Cristina)|||/items/057396d1-cf8a-4676-91fc-9100b0169390, Ecay, M. (Margarita)|||/items/62075174-6491-4648-a96e-d724a641d353, Soriano, M. (Mario)|||/items/8eae3369-3c62-4ccc-8247-03ae93b9a559, Aranda, P. (P.)|||/items/92ca618c-acbf-4ecc-99dc-498fe5af2078, Collantes, M. (María)|||/items/102ad3e7-3f57-4958-8e97-39af1c92626b, Alegria, E. (Eduardo)|||/items/66783cb2-c3ec-4c12-bed5-74d89aafeef0, Merino, J. (Juana)|||/items/e1c30c26-556a-42b9-adf9-aaae7bb3b425, Peñuelas-Sanchez, I. (Ivan)|||/items/7022e2fa-b3b0-48ed-8d39-2020cfae85a4, Garcia-Verdugo, J.M. (José Manuel)|||/items/06bcbe24-fc30-4c80-9f45-6264207191ba, Pelacho-Samper, B. (Beatriz)|||/items/aca35c0e-183d-435d-9d44-1888e0854ff3, Prosper-Cardoso, F. (Felipe)|||/items/3d1b0b82-06c3-4e63-8280-e903dc4dc0c1
Tipo de recurso: artículo
Fecha de publicación:2009
País:España
Institución:Universidad de Navarra
Repositorio:Dadun. Depósito Académico Digital de la Universidad de Navarra
Idioma:inglés
OAI Identifier:oai:dadun.unav.edu:10171/13519
Acceso en línea:https://hdl.handle.net/10171/13519
Access Level:acceso abierto
Palabra clave:Bone marrow stem cells
Angiogenesis
Chronic myocardial infarction
Cardiac remodeling
Área de Terapia Celular
Descripción
Sumario:The aim of this study is to assess the long-term effect of mesenchymal stem cells (MSC) transplantation in a rat model of chronic myocardial infarction (MI) in comparison with the effect of bone marrow mononuclear cells (BM-MNC) transplant. Five weeks after induction of MI, rats were allocated to receive intramyocardial injection of 106 GFP-expressing cells (BM-MNC or MSC) or medium as control. Heart function (echocardiography and 18F-FDG-microPET) and histological studies were performed 3 months after transplantation and cell fate was analyzed along the experiment (1 and 2 weeks and 1 and 3 months). The main findings of this study were that both BM-derived populations, BM-MNC and MSC, induced a long-lasting (3 months) improvement in LVEF (BM-MNC: 26.61 ± 2.01% to 46.61 ± 3.7%, p < 0.05; MSC: 27.5 ± 1.28% to 38.8 ± 3.2%, p < 0.05) but remarkably, only MSC improved tissue metabolism quantified by 18FFDG uptake (71.15 ± 1.27 to 76.31 ± 1.11, p < 0.01), which was thereby associated with a smaller infarct size and scar collagen content and also with a higher revascularization degree. Altogether, results show that MSC provides a long-term superior benefit than whole BM-MNC transplantation in a rat model of chronic MI.

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