Oral efficacy of a diselenide compound loaded in nanostructured lipid carriers in a murine model of visceral leishmaniasis

Leishmaniasis urgently needs new oral treatments, as it is one of the most important neglected tropical diseases that affects people with poor resources. The drug discovery pipeline for oral administration currently discards entities with poor aqueous solubility and permeability (class IV compounds...

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Autores: Etxebeste-Mitxeltorena, M. (Mikel)|||/items/ed41b569-69ef-41f9-805b-e0b9b1e49191, Moreno-Amatria, E. (Esther)|||/items/9dcce7d3-851e-4ffb-9cd0-2f07c8599f4f, Carvalheiro, M. (Manuela)|||/items/84f57a69-92e6-41c5-a3f8-e6e4af08a060, Calvo-Bacaicoa, A. (Alba)|||/items/8557046e-51a2-45a9-b289-9b6e0b1648d3, Navarro-Blasco, I. (Iñigo)|||/items/733b109b-1074-49e9-8952-70ec6928cc54, Gonzalez-Peñas, E. (Elena)|||/items/0e4b9ed7-4224-4664-a4f1-c6403a8c833f, Alvarez-Galindo, J.I. (José Ignacio)|||/items/c88ef755-513c-4ff3-bbff-44aadbf32204, Plano-Amatriain, D. (Daniel)|||/items/238881e4-7d5e-4598-924d-4392e3b7e02e, Irache-Garreta, J.M. (Juan Manuel)|||/items/c7cbbe9e-faeb-47e1-b7e8-2d956ca50173, Almeida, A.J. (Antonio J.)|||/items/bc31603c-5436-4a19-99ab-3ec476e07f2f, Sanmartin-Grijalba, C. (Carmen)|||/items/d36bd105-ab64-427d-9745-5812cf5e7af7, Espuelas, S. (Socorro)|||/items/b57c05de-0bc4-4fcf-ae1e-c9b2fb92964c
Tipo de recurso: artículo
Fecha de publicación:2021
País:España
Institución:Universidad de Navarra
Repositorio:Dadun. Depósito Académico Digital de la Universidad de Navarra
Idioma:inglés
OAI Identifier:oai:dadun.unav.edu:10171/62944
Acceso en línea:https://hdl.handle.net/10171/62944
Access Level:acceso abierto
Palabra clave:Diselenide
Nanostructured lipid carriers
Visceral leishmanisis
L. infantum
Oral treatment
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spelling Oral efficacy of a diselenide compound loaded in nanostructured lipid carriers in a murine model of visceral leishmaniasisEtxebeste-Mitxeltorena, M. (Mikel)|||/items/ed41b569-69ef-41f9-805b-e0b9b1e49191Moreno-Amatria, E. (Esther)|||/items/9dcce7d3-851e-4ffb-9cd0-2f07c8599f4fCarvalheiro, M. (Manuela)|||/items/84f57a69-92e6-41c5-a3f8-e6e4af08a060Calvo-Bacaicoa, A. (Alba)|||/items/8557046e-51a2-45a9-b289-9b6e0b1648d3Navarro-Blasco, I. (Iñigo)|||/items/733b109b-1074-49e9-8952-70ec6928cc54Gonzalez-Peñas, E. (Elena)|||/items/0e4b9ed7-4224-4664-a4f1-c6403a8c833fAlvarez-Galindo, J.I. (José Ignacio)|||/items/c88ef755-513c-4ff3-bbff-44aadbf32204Plano-Amatriain, D. (Daniel)|||/items/238881e4-7d5e-4598-924d-4392e3b7e02eIrache-Garreta, J.M. (Juan Manuel)|||/items/c7cbbe9e-faeb-47e1-b7e8-2d956ca50173Almeida, A.J. (Antonio J.)|||/items/bc31603c-5436-4a19-99ab-3ec476e07f2fSanmartin-Grijalba, C. (Carmen)|||/items/d36bd105-ab64-427d-9745-5812cf5e7af7Espuelas, S. (Socorro)|||/items/b57c05de-0bc4-4fcf-ae1e-c9b2fb92964cDiselenideNanostructured lipid carriersVisceral leishmanisisL. infantumOral treatmentLeishmaniasis urgently needs new oral treatments, as it is one of the most important neglected tropical diseases that affects people with poor resources. The drug discovery pipeline for oral administration currently discards entities with poor aqueous solubility and permeability (class IV compounds in the Biopharmaceutical Classification System, BCS) such as the diselenide 2m, a trypanothione reductase (TR) inhibitor. This work was assisted by glyceryl palmitostearate and diethylene glycol monoethyl ether-based nanostructured lipid carriers (NLC) to render 2m bioavailable and effective after its oral administration. The loading of 2m in NLC drastically enhanced its intestinal permeability and provided plasmatic levels higher than its effective concentration (IC50). In L. infantum-infected BALB/c mice, 2m-NLC reduced the parasite burden in the spleen, liver, and bone marrow by at least 95% after 5 doses, demonstrating similar efficacy as intravenous Fungizone. Overall, compound 2m and its formulation merit further investigation as an oral treatment for visceral leishmaniasis.Dadun. Depósito Académico Digital Universidad de Navarra20222022-02-1820212021-01-0120212021-01-01journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10171/62944reponame:Dadun. Depósito Académico Digital de la Universidad de Navarrainstname:Universidad de NavarraInglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:dadun.unav.edu:10171/629442026-06-21T12:47:57Z
dc.title.none.fl_str_mv Oral efficacy of a diselenide compound loaded in nanostructured lipid carriers in a murine model of visceral leishmaniasis
title Oral efficacy of a diselenide compound loaded in nanostructured lipid carriers in a murine model of visceral leishmaniasis
spellingShingle Oral efficacy of a diselenide compound loaded in nanostructured lipid carriers in a murine model of visceral leishmaniasis
Etxebeste-Mitxeltorena, M. (Mikel)|||/items/ed41b569-69ef-41f9-805b-e0b9b1e49191
Diselenide
Nanostructured lipid carriers
Visceral leishmanisis
L. infantum
Oral treatment
title_short Oral efficacy of a diselenide compound loaded in nanostructured lipid carriers in a murine model of visceral leishmaniasis
title_full Oral efficacy of a diselenide compound loaded in nanostructured lipid carriers in a murine model of visceral leishmaniasis
title_fullStr Oral efficacy of a diselenide compound loaded in nanostructured lipid carriers in a murine model of visceral leishmaniasis
title_full_unstemmed Oral efficacy of a diselenide compound loaded in nanostructured lipid carriers in a murine model of visceral leishmaniasis
title_sort Oral efficacy of a diselenide compound loaded in nanostructured lipid carriers in a murine model of visceral leishmaniasis
dc.creator.none.fl_str_mv Etxebeste-Mitxeltorena, M. (Mikel)|||/items/ed41b569-69ef-41f9-805b-e0b9b1e49191
Moreno-Amatria, E. (Esther)|||/items/9dcce7d3-851e-4ffb-9cd0-2f07c8599f4f
Carvalheiro, M. (Manuela)|||/items/84f57a69-92e6-41c5-a3f8-e6e4af08a060
Calvo-Bacaicoa, A. (Alba)|||/items/8557046e-51a2-45a9-b289-9b6e0b1648d3
Navarro-Blasco, I. (Iñigo)|||/items/733b109b-1074-49e9-8952-70ec6928cc54
Gonzalez-Peñas, E. (Elena)|||/items/0e4b9ed7-4224-4664-a4f1-c6403a8c833f
Alvarez-Galindo, J.I. (José Ignacio)|||/items/c88ef755-513c-4ff3-bbff-44aadbf32204
Plano-Amatriain, D. (Daniel)|||/items/238881e4-7d5e-4598-924d-4392e3b7e02e
Irache-Garreta, J.M. (Juan Manuel)|||/items/c7cbbe9e-faeb-47e1-b7e8-2d956ca50173
Almeida, A.J. (Antonio J.)|||/items/bc31603c-5436-4a19-99ab-3ec476e07f2f
Sanmartin-Grijalba, C. (Carmen)|||/items/d36bd105-ab64-427d-9745-5812cf5e7af7
Espuelas, S. (Socorro)|||/items/b57c05de-0bc4-4fcf-ae1e-c9b2fb92964c
author Etxebeste-Mitxeltorena, M. (Mikel)|||/items/ed41b569-69ef-41f9-805b-e0b9b1e49191
author_facet Etxebeste-Mitxeltorena, M. (Mikel)|||/items/ed41b569-69ef-41f9-805b-e0b9b1e49191
Moreno-Amatria, E. (Esther)|||/items/9dcce7d3-851e-4ffb-9cd0-2f07c8599f4f
Carvalheiro, M. (Manuela)|||/items/84f57a69-92e6-41c5-a3f8-e6e4af08a060
Calvo-Bacaicoa, A. (Alba)|||/items/8557046e-51a2-45a9-b289-9b6e0b1648d3
Navarro-Blasco, I. (Iñigo)|||/items/733b109b-1074-49e9-8952-70ec6928cc54
Gonzalez-Peñas, E. (Elena)|||/items/0e4b9ed7-4224-4664-a4f1-c6403a8c833f
Alvarez-Galindo, J.I. (José Ignacio)|||/items/c88ef755-513c-4ff3-bbff-44aadbf32204
Plano-Amatriain, D. (Daniel)|||/items/238881e4-7d5e-4598-924d-4392e3b7e02e
Irache-Garreta, J.M. (Juan Manuel)|||/items/c7cbbe9e-faeb-47e1-b7e8-2d956ca50173
Almeida, A.J. (Antonio J.)|||/items/bc31603c-5436-4a19-99ab-3ec476e07f2f
Sanmartin-Grijalba, C. (Carmen)|||/items/d36bd105-ab64-427d-9745-5812cf5e7af7
Espuelas, S. (Socorro)|||/items/b57c05de-0bc4-4fcf-ae1e-c9b2fb92964c
author_role author
author2 Moreno-Amatria, E. (Esther)|||/items/9dcce7d3-851e-4ffb-9cd0-2f07c8599f4f
Carvalheiro, M. (Manuela)|||/items/84f57a69-92e6-41c5-a3f8-e6e4af08a060
Calvo-Bacaicoa, A. (Alba)|||/items/8557046e-51a2-45a9-b289-9b6e0b1648d3
Navarro-Blasco, I. (Iñigo)|||/items/733b109b-1074-49e9-8952-70ec6928cc54
Gonzalez-Peñas, E. (Elena)|||/items/0e4b9ed7-4224-4664-a4f1-c6403a8c833f
Alvarez-Galindo, J.I. (José Ignacio)|||/items/c88ef755-513c-4ff3-bbff-44aadbf32204
Plano-Amatriain, D. (Daniel)|||/items/238881e4-7d5e-4598-924d-4392e3b7e02e
Irache-Garreta, J.M. (Juan Manuel)|||/items/c7cbbe9e-faeb-47e1-b7e8-2d956ca50173
Almeida, A.J. (Antonio J.)|||/items/bc31603c-5436-4a19-99ab-3ec476e07f2f
Sanmartin-Grijalba, C. (Carmen)|||/items/d36bd105-ab64-427d-9745-5812cf5e7af7
Espuelas, S. (Socorro)|||/items/b57c05de-0bc4-4fcf-ae1e-c9b2fb92964c
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Dadun. Depósito Académico Digital Universidad de Navarra
dc.subject.none.fl_str_mv Diselenide
Nanostructured lipid carriers
Visceral leishmanisis
L. infantum
Oral treatment
topic Diselenide
Nanostructured lipid carriers
Visceral leishmanisis
L. infantum
Oral treatment
description Leishmaniasis urgently needs new oral treatments, as it is one of the most important neglected tropical diseases that affects people with poor resources. The drug discovery pipeline for oral administration currently discards entities with poor aqueous solubility and permeability (class IV compounds in the Biopharmaceutical Classification System, BCS) such as the diselenide 2m, a trypanothione reductase (TR) inhibitor. This work was assisted by glyceryl palmitostearate and diethylene glycol monoethyl ether-based nanostructured lipid carriers (NLC) to render 2m bioavailable and effective after its oral administration. The loading of 2m in NLC drastically enhanced its intestinal permeability and provided plasmatic levels higher than its effective concentration (IC50). In L. infantum-infected BALB/c mice, 2m-NLC reduced the parasite burden in the spleen, liver, and bone marrow by at least 95% after 5 doses, demonstrating similar efficacy as intravenous Fungizone. Overall, compound 2m and its formulation merit further investigation as an oral treatment for visceral leishmaniasis.
publishDate 2021
dc.date.none.fl_str_mv 2021
2021-01-01
2021
2021-01-01
2022
2022-02-18
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/10171/62944
url https://hdl.handle.net/10171/62944
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Dadun. Depósito Académico Digital de la Universidad de Navarra
instname:Universidad de Navarra
instname_str Universidad de Navarra
reponame_str Dadun. Depósito Académico Digital de la Universidad de Navarra
collection Dadun. Depósito Académico Digital de la Universidad de Navarra
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