The use of two-dimensional electrophoresis in the characterization of the water-soluble protein fraction of commercial flat fish species

7 páginas, 2 tablas, 4 figuras.-- The original publication is available at www.springerlink.com

Detalles Bibliográficos
Autores: Piñeiro, Carmen, Barros-Velázquez, Jorge, González Sotelo, Carmen, Gallardo, José Manuel
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:1999
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/57483
Acceso en línea:http://hdl.handle.net/10261/57483
Access Level:acceso abierto
Palabra clave:Two-dimensional electrophoresis
Isoelectric focusing
Flat fish
Protein
Identification
Differential characterization
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spelling The use of two-dimensional electrophoresis in the characterization of the water-soluble protein fraction of commercial flat fish speciesPiñeiro, CarmenBarros-Velázquez, JorgeGonzález Sotelo, CarmenGallardo, José ManuelTwo-dimensional electrophoresisIsoelectric focusingFlat fishProteinIdentificationDifferential characterization7 páginas, 2 tablas, 4 figuras.-- The original publication is available at www.springerlink.comThe water-soluble proteins of nine flat fish species of high commercial value, belonging to the Pleuronectidae, Scophtalmidae, and Soleidae families, were analyzed by two-dimensional (2D) electrophoresis, this being carried out by nondenaturing isoelectric focusing (IEF) in the 3.5–9.5 pH range and gradient SDS-PAGE in the 12–14% range. Most of the major proteins fell in the 3.5–6.9 pH range. From these, the most specific proteins were in the acidic fraction (pI~5.2; MW~16 kDa). Species-specific 2D protein patterns were observed in all the nine species, and more than 25 proteins could be individualized. The combination of nondenaturing IEF and gradient SDSPAGE, both in the absence of urea, allowed detailed characterization of both the isoelectric points and molecular weights of the major water-soluble proteins and proved to be a valuable tool for the differential characterization of the flat fish species studied.The authors thank the financial support of CICYT (Project ALI95–0053).Peer reviewedSpringer NatureComisión Interministerial de Ciencia y Tecnología, CICYT (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201220121999info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/57483reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1007/s002170050427Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/574832026-05-22T06:33:51Z
dc.title.none.fl_str_mv The use of two-dimensional electrophoresis in the characterization of the water-soluble protein fraction of commercial flat fish species
title The use of two-dimensional electrophoresis in the characterization of the water-soluble protein fraction of commercial flat fish species
spellingShingle The use of two-dimensional electrophoresis in the characterization of the water-soluble protein fraction of commercial flat fish species
Piñeiro, Carmen
Two-dimensional electrophoresis
Isoelectric focusing
Flat fish
Protein
Identification
Differential characterization
title_short The use of two-dimensional electrophoresis in the characterization of the water-soluble protein fraction of commercial flat fish species
title_full The use of two-dimensional electrophoresis in the characterization of the water-soluble protein fraction of commercial flat fish species
title_fullStr The use of two-dimensional electrophoresis in the characterization of the water-soluble protein fraction of commercial flat fish species
title_full_unstemmed The use of two-dimensional electrophoresis in the characterization of the water-soluble protein fraction of commercial flat fish species
title_sort The use of two-dimensional electrophoresis in the characterization of the water-soluble protein fraction of commercial flat fish species
dc.creator.none.fl_str_mv Piñeiro, Carmen
Barros-Velázquez, Jorge
González Sotelo, Carmen
Gallardo, José Manuel
author Piñeiro, Carmen
author_facet Piñeiro, Carmen
Barros-Velázquez, Jorge
González Sotelo, Carmen
Gallardo, José Manuel
author_role author
author2 Barros-Velázquez, Jorge
González Sotelo, Carmen
Gallardo, José Manuel
author2_role author
author
author
dc.contributor.none.fl_str_mv Comisión Interministerial de Ciencia y Tecnología, CICYT (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Two-dimensional electrophoresis
Isoelectric focusing
Flat fish
Protein
Identification
Differential characterization
topic Two-dimensional electrophoresis
Isoelectric focusing
Flat fish
Protein
Identification
Differential characterization
description 7 páginas, 2 tablas, 4 figuras.-- The original publication is available at www.springerlink.com
publishDate 1999
dc.date.none.fl_str_mv 1999
2012
2012
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/57483
url http://hdl.handle.net/10261/57483
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1007/s002170050427
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Springer Nature
publisher.none.fl_str_mv Springer Nature
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,81155

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