Bacteriophage adsorption on electrospun scaffolds of PLA loaded with cationic arginine compounds

The research about biodegradable polymers has grown so quickly during last decades. One of the main reasons is their application in the field of biomedicine. These polymers are nontoxic for the organism and could be metabolized by the human body after a controlled degradation. Gene therapy success d...

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Detalhes bibliográficos
Autor: Lorenzo Abril, Alejandro
Formato: tesis de maestría
Fecha de publicación:2015
País:España
Recursos:Universitat Politècnica de Catalunya (UPC)
Repositorio:UPCommons. Portal del coneixement obert de la UPC
Idioma:inglés
OAI Identifier:oai:upcommons.upc.edu:2117/77347
Acesso em linha:https://hdl.handle.net/2117/77347
Access Level:acceso abierto
Palavra-chave:Tissue scaffolds
Bacteriophages
Electrospinning
Biopolímers -- Microestructura
Teixits -- Bastides
Bacteriòfags
Electrofilatura
Biopolymers -- Microstructure
Àrees temàtiques de la UPC::Enginyeria biomèdica::Biomaterials
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spelling Bacteriophage adsorption on electrospun scaffolds of PLA loaded with cationic arginine compoundsLorenzo Abril, AlejandroTissue scaffoldsBacteriophagesElectrospinningBiopolímers -- MicroestructuraTeixits -- BastidesBacteriòfagsElectrofilaturaBiopolymers -- MicrostructureÀrees temàtiques de la UPC::Enginyeria biomèdica::BiomaterialsThe research about biodegradable polymers has grown so quickly during last decades. One of the main reasons is their application in the field of biomedicine. These polymers are nontoxic for the organism and could be metabolized by the human body after a controlled degradation. Gene therapy success depends on safety and effective of the gene carriers. For that reason the creation of new synthetic vectors is necessary. The temporary scaffolds are one of the most important applications of biodegradable polymers. Temporary scaffolds are used to replace a tissue of the human body that has been broken or weakened by an illness, injury or surgery. In this project new synthetized biodegradable polymers based on arginine are employed and characterized through FTIR, RMN, DSC, X-rays and GPC. Electrospun PLA scaffolds containing either this new family of polymers or pure arginine are prepared. To obtain the optimal quality of the scaffold it is necessary to tune the electrospinning operational parameters (e.g. flow rate, voltage, needle-collector distance, collector type). Furthermore, both the conditions of the solution (e.g. polymer type and concentration, viscosity, conductivity) as well as the ambient parameters play an important role to get the optimal electrospinning conditions. The scaffolds are characterized with different techniques, SEM for the morphologic study and fiber diameter measurement, contact angle to evaluate the hydrophobicity of the surface, infrared analysis to assess the addition of the different arginine compounds, and thermal analysis to determine variations on thermal stability and characteristic transition temperatures. The use of bacteriophages is an interesting alternative for drug-resistant infections that has shown many good results in recent studies. The bacteriophages are a type of virus that only infects bacteria. Once characterized, the new prepared electrospun mats are loaded by adsorption with bacteriophages, which are specific for the Staphylococcus Aureus bacteria. The antibacterial ability of the loaded scaffolds is tested. PHMB is a well-known antibacterial compound that is used as positive control. Biocompatibility of new samples is also tested through cell adhesion and proliferation studies. Finally, the potential use in biomedicine for tissue engineering and infection control is evaluated.Universitat Politècnica de CatalunyaValle Mendoza, Luis Javier delPuiggalí Bellalta, Jordi20152015-06-0120152015-10-05master thesishttp://purl.org/coar/resource_type/c_bdccNAhttp://purl.org/coar/version/c_be7fb7dd8ff6fe43info:eu-repo/semantics/masterThesisapplication/pdfhttps://hdl.handle.net/2117/77347reponame:UPCommons. Portal del coneixement obert de la UPCinstname:Universitat Politècnica de Catalunya (UPC)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivs 3.0 Spainhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/info:eu-repo/semantics/openAccessoai:upcommons.upc.edu:2117/773472026-05-27T15:37:01Z
dc.title.none.fl_str_mv Bacteriophage adsorption on electrospun scaffolds of PLA loaded with cationic arginine compounds
title Bacteriophage adsorption on electrospun scaffolds of PLA loaded with cationic arginine compounds
spellingShingle Bacteriophage adsorption on electrospun scaffolds of PLA loaded with cationic arginine compounds
Lorenzo Abril, Alejandro
Tissue scaffolds
Bacteriophages
Electrospinning
Biopolímers -- Microestructura
Teixits -- Bastides
Bacteriòfags
Electrofilatura
Biopolymers -- Microstructure
Àrees temàtiques de la UPC::Enginyeria biomèdica::Biomaterials
title_short Bacteriophage adsorption on electrospun scaffolds of PLA loaded with cationic arginine compounds
title_full Bacteriophage adsorption on electrospun scaffolds of PLA loaded with cationic arginine compounds
title_fullStr Bacteriophage adsorption on electrospun scaffolds of PLA loaded with cationic arginine compounds
title_full_unstemmed Bacteriophage adsorption on electrospun scaffolds of PLA loaded with cationic arginine compounds
title_sort Bacteriophage adsorption on electrospun scaffolds of PLA loaded with cationic arginine compounds
dc.creator.none.fl_str_mv Lorenzo Abril, Alejandro
author Lorenzo Abril, Alejandro
author_facet Lorenzo Abril, Alejandro
author_role author
dc.contributor.none.fl_str_mv Valle Mendoza, Luis Javier del
Puiggalí Bellalta, Jordi
dc.subject.none.fl_str_mv Tissue scaffolds
Bacteriophages
Electrospinning
Biopolímers -- Microestructura
Teixits -- Bastides
Bacteriòfags
Electrofilatura
Biopolymers -- Microstructure
Àrees temàtiques de la UPC::Enginyeria biomèdica::Biomaterials
topic Tissue scaffolds
Bacteriophages
Electrospinning
Biopolímers -- Microestructura
Teixits -- Bastides
Bacteriòfags
Electrofilatura
Biopolymers -- Microstructure
Àrees temàtiques de la UPC::Enginyeria biomèdica::Biomaterials
description The research about biodegradable polymers has grown so quickly during last decades. One of the main reasons is their application in the field of biomedicine. These polymers are nontoxic for the organism and could be metabolized by the human body after a controlled degradation. Gene therapy success depends on safety and effective of the gene carriers. For that reason the creation of new synthetic vectors is necessary. The temporary scaffolds are one of the most important applications of biodegradable polymers. Temporary scaffolds are used to replace a tissue of the human body that has been broken or weakened by an illness, injury or surgery. In this project new synthetized biodegradable polymers based on arginine are employed and characterized through FTIR, RMN, DSC, X-rays and GPC. Electrospun PLA scaffolds containing either this new family of polymers or pure arginine are prepared. To obtain the optimal quality of the scaffold it is necessary to tune the electrospinning operational parameters (e.g. flow rate, voltage, needle-collector distance, collector type). Furthermore, both the conditions of the solution (e.g. polymer type and concentration, viscosity, conductivity) as well as the ambient parameters play an important role to get the optimal electrospinning conditions. The scaffolds are characterized with different techniques, SEM for the morphologic study and fiber diameter measurement, contact angle to evaluate the hydrophobicity of the surface, infrared analysis to assess the addition of the different arginine compounds, and thermal analysis to determine variations on thermal stability and characteristic transition temperatures. The use of bacteriophages is an interesting alternative for drug-resistant infections that has shown many good results in recent studies. The bacteriophages are a type of virus that only infects bacteria. Once characterized, the new prepared electrospun mats are loaded by adsorption with bacteriophages, which are specific for the Staphylococcus Aureus bacteria. The antibacterial ability of the loaded scaffolds is tested. PHMB is a well-known antibacterial compound that is used as positive control. Biocompatibility of new samples is also tested through cell adhesion and proliferation studies. Finally, the potential use in biomedicine for tissue engineering and infection control is evaluated.
publishDate 2015
dc.date.none.fl_str_mv 2015
2015-06-01
2015
2015-10-05
dc.type.none.fl_str_mv master thesis
http://purl.org/coar/resource_type/c_bdcc
NA
http://purl.org/coar/version/c_be7fb7dd8ff6fe43
dc.type.openaire.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
dc.identifier.none.fl_str_mv https://hdl.handle.net/2117/77347
url https://hdl.handle.net/2117/77347
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivs 3.0 Spain
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivs 3.0 Spain
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universitat Politècnica de Catalunya
publisher.none.fl_str_mv Universitat Politècnica de Catalunya
dc.source.none.fl_str_mv reponame:UPCommons. Portal del coneixement obert de la UPC
instname:Universitat Politècnica de Catalunya (UPC)
instname_str Universitat Politècnica de Catalunya (UPC)
reponame_str UPCommons. Portal del coneixement obert de la UPC
collection UPCommons. Portal del coneixement obert de la UPC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,301603

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