Rheology and bioactivity of high molecular weight dextrans synt hesised by lactic acid bacteria

36 p.-6 fig.-1 tab.

Detalles Bibliográficos
Autores: Zarour, Kenza, Llamas, M. G., Prieto Orzanco, Alicia, Ruas-Madiedo, Patricia, Dueñas, María Teresa, Fernández de Palencia, P., Aznar, Rosa, Kihal, Mebrouk, López García, Paloma
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2017
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/152435
Acceso en línea:http://hdl.handle.net/10261/152435
Access Level:acceso abierto
Palabra clave:Exopolysaccharides
Dextran
Lactic acid bacteria
Leuconostoc mesenteroides
Rheological properties
Immunomodulation
Adhesion
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spelling Rheology and bioactivity of high molecular weight dextrans synt hesised by lactic acid bacteriaZarour, KenzaLlamas, M. G.Prieto Orzanco, AliciaRuas-Madiedo, PatriciaDueñas, María TeresaFernández de Palencia, P.Aznar, RosaKihal, MebroukLópez García, PalomaExopolysaccharidesDextranLactic acid bacteriaLeuconostoc mesenteroidesRheological propertiesImmunomodulationAdhesion36 p.-6 fig.-1 tab.Dextrans synthesised by three Leuconostoc mesenteroides strains, isolated from mammalian milks, were studied and compared with dextrans produced by Lc. mesenteroides and Lactobacillus sakei strains isolated from meat products. Size exclusion chromatography coupled with multiangle laser light scattering detection analysis demonstrated that the dextrans have molecular masses between 1.74 × 108 Da and 4.41 × 108 Da. Rheological analysis of aqueous solutions of the polymer revealed that all had a pseudoplastic behaviour under shear conditions and a random, and flexible, coil structure. The dextrans showed at shear zero a difference in viscosity, which increased as the concentration increased. Also, the purified dextrans were able to immunomodulate in vitro human macrophages, partially counteracting the inflammatory effect of Escherichia coli O111:B4 lipopolysaccharide. During prolonged incubation on a solid medium containing sucrose, dextran-producing bacteria showed two distinct phenotypes not related to the genus or species to which they belonged. Colonies of Lc. mesenteroides CM9 from milk and Lb. sakei MN1 from meat formed stable and compact mucoid colonies, whereas the colonies of the other three Leuconostoc strains became diffuse after 72 h. This differential behaviour was also observed in the ability of the corresponding strains to bind to Caco-2 cells. Strains forming compact mucoid colonies showed a high level of adhesion when grown in the presence of glucose, which decreased in the presence of sucrose (the condition required for dextran synthesis). However no influence of the carbon source was detected for the adhesion ability of the other Lc. mesenteroides strains, which showed variable levels of binding to the enterocytes.This work was supported by the Spanish Ministry of Economy and Competitiveness (grant AGL2015-65010-C3-1-R).Peer reviewedElsevierMinisterio de Economía y Competitividad (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201720172017info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/152435reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2015-65010-C3-1-Rhttps://doi.org/10.1016/j.carbpol.2017.06.113Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1524352026-05-22T06:33:51Z
dc.title.none.fl_str_mv Rheology and bioactivity of high molecular weight dextrans synt hesised by lactic acid bacteria
title Rheology and bioactivity of high molecular weight dextrans synt hesised by lactic acid bacteria
spellingShingle Rheology and bioactivity of high molecular weight dextrans synt hesised by lactic acid bacteria
Zarour, Kenza
Exopolysaccharides
Dextran
Lactic acid bacteria
Leuconostoc mesenteroides
Rheological properties
Immunomodulation
Adhesion
title_short Rheology and bioactivity of high molecular weight dextrans synt hesised by lactic acid bacteria
title_full Rheology and bioactivity of high molecular weight dextrans synt hesised by lactic acid bacteria
title_fullStr Rheology and bioactivity of high molecular weight dextrans synt hesised by lactic acid bacteria
title_full_unstemmed Rheology and bioactivity of high molecular weight dextrans synt hesised by lactic acid bacteria
title_sort Rheology and bioactivity of high molecular weight dextrans synt hesised by lactic acid bacteria
dc.creator.none.fl_str_mv Zarour, Kenza
Llamas, M. G.
Prieto Orzanco, Alicia
Ruas-Madiedo, Patricia
Dueñas, María Teresa
Fernández de Palencia, P.
Aznar, Rosa
Kihal, Mebrouk
López García, Paloma
author Zarour, Kenza
author_facet Zarour, Kenza
Llamas, M. G.
Prieto Orzanco, Alicia
Ruas-Madiedo, Patricia
Dueñas, María Teresa
Fernández de Palencia, P.
Aznar, Rosa
Kihal, Mebrouk
López García, Paloma
author_role author
author2 Llamas, M. G.
Prieto Orzanco, Alicia
Ruas-Madiedo, Patricia
Dueñas, María Teresa
Fernández de Palencia, P.
Aznar, Rosa
Kihal, Mebrouk
López García, Paloma
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Exopolysaccharides
Dextran
Lactic acid bacteria
Leuconostoc mesenteroides
Rheological properties
Immunomodulation
Adhesion
topic Exopolysaccharides
Dextran
Lactic acid bacteria
Leuconostoc mesenteroides
Rheological properties
Immunomodulation
Adhesion
description 36 p.-6 fig.-1 tab.
publishDate 2017
dc.date.none.fl_str_mv 2017
2017
2017
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/152435
url http://hdl.handle.net/10261/152435
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2015-65010-C3-1-R
https://doi.org/10.1016/j.carbpol.2017.06.113
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
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