In vitro generation of novel functionalized biomaterials for use in oral and dental regenerative medicine applications. Running title: Fibrin-agarose functionalized scaffolds
Recent advances in tissue engineering offer innovative clinical alternatives in dentistry and regenerative medicine. Tissue engineering combines human cells with compatible biomaterials to induce tissue regeneration. Shortening the fabrication time of biomaterials used in tissue engineering will con...
| Autores: | , , , , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Fecha de publicación: | 2020 |
| País: | España |
| Institución: | Universitat Politècnica de Catalunya (UPC) |
| Repositorio: | UPCommons. Portal del coneixement obert de la UPC |
| Idioma: | inglés |
| OAI Identifier: | oai:upcommons.upc.edu:2117/330460 |
| Acceso en línea: | https://hdl.handle.net/2117/330460 https://dx.doi.org/10.3390/ma13071692 |
| Access Level: | acceso abierto |
| Palabra clave: | Biomedical materials Tissue engineering Dental implants Functionalization Oral and dental tissues Biomaterials Extracellular matrix Materials biomèdics Enginyeria de teixits Implants dentals Àrees temàtiques de la UPC::Enginyeria dels materials |
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In vitro generation of novel functionalized biomaterials for use in oral and dental regenerative medicine applications. Running title: Fibrin-agarose functionalized scaffoldsBlanco Elices, CristinaEspaña Guerrero, EnriqueMateu Sanz, Miguel|||0000-0001-5117-6071Sánchez Porras, DavidGarcía García, Oscar DaríoSanchéz Quevedo, Maria del CarmenFernández Valadés, RicardoAlaminos Mingorance, MiguelMartín Piedra, Miguel AngelGarzón Bello, Ingrid JohannaBiomedical materialsTissue engineeringDental implantsFunctionalizationOral and dental tissuesBiomaterialsExtracellular matrixTissue engineeringMaterials biomèdicsEnginyeria de teixitsImplants dentalsÀrees temàtiques de la UPC::Enginyeria dels materialsRecent advances in tissue engineering offer innovative clinical alternatives in dentistry and regenerative medicine. Tissue engineering combines human cells with compatible biomaterials to induce tissue regeneration. Shortening the fabrication time of biomaterials used in tissue engineering will contribute to treatment improvement, and biomaterial functionalization can be exploited to enhance scaffold properties. In this work, we have tested an alternative biofabrication method by directly including human oral mucosa tissue explants within the biomaterial for the generation of human bioengineered mouth and dental tissues for use in tissue engineering. To achieve this, acellular fibrin–agarose scaffolds (AFAS), non-functionalized fibrin-agarose oral mucosa stroma substitutes (n-FAOM), and novel functionalized fibrin-agarose oral mucosa stroma substitutes (F-FAOM) were developed and analyzed after 1, 2, and 3 weeks of in vitro development to determine extracellular matrix components as compared to native oral mucosa controls by using histochemistry and immunohistochemistry. Results demonstrate that functionalization speeds up the biofabrication method and contributes to improve the biomimetic characteristics of the scaffold in terms of extracellular matrix components and reduce the time required for in vitro tissue developmentPeer ReviewedMultidisciplinary Digital Publishing Institute (MDPI)20202020-04-0120202020-10-20journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/2117/330460https://dx.doi.org/10.3390/ma13071692reponame:UPCommons. Portal del coneixement obert de la UPCinstname:Universitat Politècnica de Catalunya (UPC)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivs 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:upcommons.upc.edu:2117/3304602026-05-27T15:37:01Z |
| dc.title.none.fl_str_mv |
In vitro generation of novel functionalized biomaterials for use in oral and dental regenerative medicine applications. Running title: Fibrin-agarose functionalized scaffolds |
| title |
In vitro generation of novel functionalized biomaterials for use in oral and dental regenerative medicine applications. Running title: Fibrin-agarose functionalized scaffolds |
| spellingShingle |
In vitro generation of novel functionalized biomaterials for use in oral and dental regenerative medicine applications. Running title: Fibrin-agarose functionalized scaffolds Blanco Elices, Cristina Biomedical materials Tissue engineering Dental implants Functionalization Oral and dental tissues Biomaterials Extracellular matrix Tissue engineering Materials biomèdics Enginyeria de teixits Implants dentals Àrees temàtiques de la UPC::Enginyeria dels materials |
| title_short |
In vitro generation of novel functionalized biomaterials for use in oral and dental regenerative medicine applications. Running title: Fibrin-agarose functionalized scaffolds |
| title_full |
In vitro generation of novel functionalized biomaterials for use in oral and dental regenerative medicine applications. Running title: Fibrin-agarose functionalized scaffolds |
| title_fullStr |
In vitro generation of novel functionalized biomaterials for use in oral and dental regenerative medicine applications. Running title: Fibrin-agarose functionalized scaffolds |
| title_full_unstemmed |
In vitro generation of novel functionalized biomaterials for use in oral and dental regenerative medicine applications. Running title: Fibrin-agarose functionalized scaffolds |
| title_sort |
In vitro generation of novel functionalized biomaterials for use in oral and dental regenerative medicine applications. Running title: Fibrin-agarose functionalized scaffolds |
| dc.creator.none.fl_str_mv |
Blanco Elices, Cristina España Guerrero, Enrique Mateu Sanz, Miguel|||0000-0001-5117-6071 Sánchez Porras, David García García, Oscar Darío Sanchéz Quevedo, Maria del Carmen Fernández Valadés, Ricardo Alaminos Mingorance, Miguel Martín Piedra, Miguel Angel Garzón Bello, Ingrid Johanna |
| author |
Blanco Elices, Cristina |
| author_facet |
Blanco Elices, Cristina España Guerrero, Enrique Mateu Sanz, Miguel|||0000-0001-5117-6071 Sánchez Porras, David García García, Oscar Darío Sanchéz Quevedo, Maria del Carmen Fernández Valadés, Ricardo Alaminos Mingorance, Miguel Martín Piedra, Miguel Angel Garzón Bello, Ingrid Johanna |
| author_role |
author |
| author2 |
España Guerrero, Enrique Mateu Sanz, Miguel|||0000-0001-5117-6071 Sánchez Porras, David García García, Oscar Darío Sanchéz Quevedo, Maria del Carmen Fernández Valadés, Ricardo Alaminos Mingorance, Miguel Martín Piedra, Miguel Angel Garzón Bello, Ingrid Johanna |
| author2_role |
author author author author author author author author author |
| dc.subject.none.fl_str_mv |
Biomedical materials Tissue engineering Dental implants Functionalization Oral and dental tissues Biomaterials Extracellular matrix Tissue engineering Materials biomèdics Enginyeria de teixits Implants dentals Àrees temàtiques de la UPC::Enginyeria dels materials |
| topic |
Biomedical materials Tissue engineering Dental implants Functionalization Oral and dental tissues Biomaterials Extracellular matrix Tissue engineering Materials biomèdics Enginyeria de teixits Implants dentals Àrees temàtiques de la UPC::Enginyeria dels materials |
| description |
Recent advances in tissue engineering offer innovative clinical alternatives in dentistry and regenerative medicine. Tissue engineering combines human cells with compatible biomaterials to induce tissue regeneration. Shortening the fabrication time of biomaterials used in tissue engineering will contribute to treatment improvement, and biomaterial functionalization can be exploited to enhance scaffold properties. In this work, we have tested an alternative biofabrication method by directly including human oral mucosa tissue explants within the biomaterial for the generation of human bioengineered mouth and dental tissues for use in tissue engineering. To achieve this, acellular fibrin–agarose scaffolds (AFAS), non-functionalized fibrin-agarose oral mucosa stroma substitutes (n-FAOM), and novel functionalized fibrin-agarose oral mucosa stroma substitutes (F-FAOM) were developed and analyzed after 1, 2, and 3 weeks of in vitro development to determine extracellular matrix components as compared to native oral mucosa controls by using histochemistry and immunohistochemistry. Results demonstrate that functionalization speeds up the biofabrication method and contributes to improve the biomimetic characteristics of the scaffold in terms of extracellular matrix components and reduce the time required for in vitro tissue development |
| publishDate |
2020 |
| dc.date.none.fl_str_mv |
2020 2020-04-01 2020 2020-10-20 |
| dc.type.none.fl_str_mv |
journal article http://purl.org/coar/resource_type/c_6501 VoR http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| dc.type.openaire.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| dc.identifier.none.fl_str_mv |
https://hdl.handle.net/2117/330460 https://dx.doi.org/10.3390/ma13071692 |
| url |
https://hdl.handle.net/2117/330460 https://dx.doi.org/10.3390/ma13071692 |
| dc.language.none.fl_str_mv |
Inglés eng |
| language_invalid_str_mv |
Inglés |
| language |
eng |
| dc.rights.none.fl_str_mv |
open access http://purl.org/coar/access_right/c_abf2 Attribution-NonCommercial-NoDerivs 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| dc.rights.openaire.fl_str_mv |
info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
open access http://purl.org/coar/access_right/c_abf2 Attribution-NonCommercial-NoDerivs 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
application/pdf |
| dc.publisher.none.fl_str_mv |
Multidisciplinary Digital Publishing Institute (MDPI) |
| publisher.none.fl_str_mv |
Multidisciplinary Digital Publishing Institute (MDPI) |
| dc.source.none.fl_str_mv |
reponame:UPCommons. Portal del coneixement obert de la UPC instname:Universitat Politècnica de Catalunya (UPC) |
| instname_str |
Universitat Politècnica de Catalunya (UPC) |
| reponame_str |
UPCommons. Portal del coneixement obert de la UPC |
| collection |
UPCommons. Portal del coneixement obert de la UPC |
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15.301603 |