Whole-genome snp analysis identifies putative mycobacterium bovis transmission clusters in livestock and wildlife in Catalonia, Spain

The high-resolution WGS analyses of MTBC strains have provided useful insight for determining sources of infection for animal tuberculosis. In Spain, tuberculosis in livestock is caused by Mycobacterium bovis and Mycobacterium caprae, where wildlife reservoirs play an important role. We analyzed a s...

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Autores: Perea, Claudia|||0000-0002-4863-6863, Ciaravino, Giovanna|||0000-0002-5796-8093, Stuber, Tod|||0000-0001-6097-6388, Thacker, Tyler C., Robbe-Austerman, Suelee|||0000-0002-3794-3040, Allepuz Palau, Alberto|||0000-0003-3518-1991, Pérez de Val, Bernat|||0000-0003-3127-9182
Tipo de documento: artigo
Data de publicação:2021
País:España
Recursos:Universitat Autònoma de Barcelona
Repositório:Dipòsit Digital de Documents de la UAB
Idioma:inglês
OAI Identifier:oai:ddd.uab.cat:251150
Acesso em linha:https://ddd.uab.cat/record/251150
https://dx.doi.org/urn:doi:10.3390/microorganisms9081629
Access Level:Acceso aberto
Palavra-chave:Whole-genome sequencing
SNP
Mycobacterium bovis
Bovine tuberculosis
Catalonia
Northeastern Spain
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spelling Whole-genome snp analysis identifies putative mycobacterium bovis transmission clusters in livestock and wildlife in Catalonia, SpainPerea, Claudia|||0000-0002-4863-6863Ciaravino, Giovanna|||0000-0002-5796-8093Stuber, Tod|||0000-0001-6097-6388Thacker, Tyler C.Robbe-Austerman, Suelee|||0000-0002-3794-3040Allepuz Palau, Alberto|||0000-0003-3518-1991Pérez de Val, Bernat|||0000-0003-3127-9182Whole-genome sequencingSNPMycobacterium bovisBovine tuberculosisCataloniaNortheastern SpainThe high-resolution WGS analyses of MTBC strains have provided useful insight for determining sources of infection for animal tuberculosis. In Spain, tuberculosis in livestock is caused by Mycobacterium bovis and Mycobacterium caprae, where wildlife reservoirs play an important role. We analyzed a set of 125 M. bovis isolates obtained from livestock and wildlife from Catalonia to investigate strain diversity and identify possible sources and/or causes of infection. Whole-genome SNP profiles were used for phylogenetic reconstruction and pairwise SNP distance analysis. Additionally, SNPs were investigated to identify virulence and antimicrobial resistance factors to investigate clade-specific associations. Putative transmission clusters (≤12 SNPs) were identified, and associated epidemiological metadata were used to determine possible explanatory factors for transmission. M. bovis distribution was heterogeneous, with 7 major clades and 21 putative transmission clusters. In order of importance, the explanatory factors associated were proximity and neighborhood, residual infection, livestock-wildlife interaction, shared pasture, and movement. Genes related to lipid transport and metabolism showed the highest number of SNPs. All isolates were pyrazinamide resistant, and five were additionally resistant to isoniazid, but no clade-specific associations could be determined. Our findings highlight the importance of high-resolution molecular surveillance to monitor bovine tuberculosis dynamics in a low-prevalence setting. 22021-01-0120212021-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/251150https://dx.doi.org/urn:doi:10.3390/microorganisms9081629reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengMinisterio de Economía y Competitividad https://doi.org/10.13039/501100003329 AGL2013-49159-C2-1-Ropen accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:2511502026-06-06T12:50:31Z
dc.title.none.fl_str_mv Whole-genome snp analysis identifies putative mycobacterium bovis transmission clusters in livestock and wildlife in Catalonia, Spain
title Whole-genome snp analysis identifies putative mycobacterium bovis transmission clusters in livestock and wildlife in Catalonia, Spain
spellingShingle Whole-genome snp analysis identifies putative mycobacterium bovis transmission clusters in livestock and wildlife in Catalonia, Spain
Perea, Claudia|||0000-0002-4863-6863
Whole-genome sequencing
SNP
Mycobacterium bovis
Bovine tuberculosis
Catalonia
Northeastern Spain
title_short Whole-genome snp analysis identifies putative mycobacterium bovis transmission clusters in livestock and wildlife in Catalonia, Spain
title_full Whole-genome snp analysis identifies putative mycobacterium bovis transmission clusters in livestock and wildlife in Catalonia, Spain
title_fullStr Whole-genome snp analysis identifies putative mycobacterium bovis transmission clusters in livestock and wildlife in Catalonia, Spain
title_full_unstemmed Whole-genome snp analysis identifies putative mycobacterium bovis transmission clusters in livestock and wildlife in Catalonia, Spain
title_sort Whole-genome snp analysis identifies putative mycobacterium bovis transmission clusters in livestock and wildlife in Catalonia, Spain
dc.creator.none.fl_str_mv Perea, Claudia|||0000-0002-4863-6863
Ciaravino, Giovanna|||0000-0002-5796-8093
Stuber, Tod|||0000-0001-6097-6388
Thacker, Tyler C.
Robbe-Austerman, Suelee|||0000-0002-3794-3040
Allepuz Palau, Alberto|||0000-0003-3518-1991
Pérez de Val, Bernat|||0000-0003-3127-9182
author Perea, Claudia|||0000-0002-4863-6863
author_facet Perea, Claudia|||0000-0002-4863-6863
Ciaravino, Giovanna|||0000-0002-5796-8093
Stuber, Tod|||0000-0001-6097-6388
Thacker, Tyler C.
Robbe-Austerman, Suelee|||0000-0002-3794-3040
Allepuz Palau, Alberto|||0000-0003-3518-1991
Pérez de Val, Bernat|||0000-0003-3127-9182
author_role author
author2 Ciaravino, Giovanna|||0000-0002-5796-8093
Stuber, Tod|||0000-0001-6097-6388
Thacker, Tyler C.
Robbe-Austerman, Suelee|||0000-0002-3794-3040
Allepuz Palau, Alberto|||0000-0003-3518-1991
Pérez de Val, Bernat|||0000-0003-3127-9182
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Whole-genome sequencing
SNP
Mycobacterium bovis
Bovine tuberculosis
Catalonia
Northeastern Spain
topic Whole-genome sequencing
SNP
Mycobacterium bovis
Bovine tuberculosis
Catalonia
Northeastern Spain
description The high-resolution WGS analyses of MTBC strains have provided useful insight for determining sources of infection for animal tuberculosis. In Spain, tuberculosis in livestock is caused by Mycobacterium bovis and Mycobacterium caprae, where wildlife reservoirs play an important role. We analyzed a set of 125 M. bovis isolates obtained from livestock and wildlife from Catalonia to investigate strain diversity and identify possible sources and/or causes of infection. Whole-genome SNP profiles were used for phylogenetic reconstruction and pairwise SNP distance analysis. Additionally, SNPs were investigated to identify virulence and antimicrobial resistance factors to investigate clade-specific associations. Putative transmission clusters (≤12 SNPs) were identified, and associated epidemiological metadata were used to determine possible explanatory factors for transmission. M. bovis distribution was heterogeneous, with 7 major clades and 21 putative transmission clusters. In order of importance, the explanatory factors associated were proximity and neighborhood, residual infection, livestock-wildlife interaction, shared pasture, and movement. Genes related to lipid transport and metabolism showed the highest number of SNPs. All isolates were pyrazinamide resistant, and five were additionally resistant to isoniazid, but no clade-specific associations could be determined. Our findings highlight the importance of high-resolution molecular surveillance to monitor bovine tuberculosis dynamics in a low-prevalence setting.
publishDate 2021
dc.date.none.fl_str_mv 2
2021-01-01
2021
2021-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/251150
https://dx.doi.org/urn:doi:10.3390/microorganisms9081629
url https://ddd.uab.cat/record/251150
https://dx.doi.org/urn:doi:10.3390/microorganisms9081629
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 AGL2013-49159-C2-1-R
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
collection Dipòsit Digital de Documents de la UAB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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