MicroRNA-1291 Is Associated With Locoregional Metastases in Patients With Early-Stage Breast Cancer
Evidence that microRNAs (miRNAs) regulate the various steps of metastasis is increasing. Several studies have looked at the miRNA expression profile in primary breast tumors but few have compared primary tumor and sentinel lymph node (SLN) metastasis. We correlated the expression of miRNAs with the...
| Autores: | , , , , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2020 |
| País: | España |
| Institución: | Institut d’Investigació Biomèdica Sant Pau (IIB Sant Pau) |
| Repositorio: | r-IIB SANT PAU. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica Sant Pau |
| OAI Identifier: | oai:iibsantpau.fundanetsuite.com:p11744 |
| Acceso en línea: | https://iibsantpau.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=11744 https://www.scopus.com/inward/record.uri?eid=2-s2.0-85097675801&doi=10.3389%2ffgene.2020.562114&partnerID=40&md5=a774592a8cb6d63785aa5ef57085eba2 |
| Access Level: | acceso abierto |
| Palabra clave: | cytokeratin 19 epidermal growth factor receptor 2 estrogen receptor Ki 67 antigen microRNA microRNA 1291 microRNA 182 microRNA 3535 microRNA 3607 microRNA 3651 microRNA 3653 microRNA 6240 microRNA 6516 microRNA 7641 progesterone receptor protein tyrosine kinase tumor marker unclassified drug adult Article axillary lymph node dissection bioinformatics breast cancer cancer grading cancer staging controlled study early cancer female gene expression profiling gene targeting high throughput sequencing histopathology human human tissue lymph node metastasis major clinical study middle aged non-canonical Wnt signaling real time polymerase chain reaction RNA sequencing sentinel lymph node metastasis signal transduction tumor suppressor gene |
| Sumario: | Evidence that microRNAs (miRNAs) regulate the various steps of metastasis is increasing. Several studies have looked at the miRNA expression profile in primary breast tumors but few have compared primary tumor and sentinel lymph node (SLN) metastasis. We correlated the expression of miRNAs with the SLN status and the outcome of axillary lymph node dissection (ALND) in 60 patients with early breast cancer. We profiled the expression of miRNAs in paired breast tumor samples and SLNs using the NextSeq500 Illumina platform and key findings were validated by qPCR. MultiMiR Bioconductor and Reactome pathways analysis were performed to identify target genes and signaling pathways affected by altered expressed miRNAs. Our results show that nine miRNAs were differentially expressed in tumor tissues (q = 0.05). In tumor samples, a 13.5-fold up-regulation of miR-7641-2 (q < 0.001) and a 2.9-fold down-regulation of miR-1291 (q < 0.001) were associated with tumors with positive SLNs. However, only down-regulation of miR-1291 (q = 0.048) remained significant in paired SLNs samples. Interestingly, a 10.5 up-regulation of miR-1291 in SLNs samples was associated with additional axillary lymph node involvement (q < 0.001). The enrichment analyses showed that canonical and non-canonical WNT pathways and negative regulation of various receptor tyrosine kinases signaling pathways were targets of miR-1291 and supports the role of miR-1291 as a tumor suppressor gene (TSG). Further studies are warranted to investigate the use of miR-1291 as a surrogate biomarker of SLN node metastasis in patients with early-stage breast cancer. © Copyright © 2020 Escuin, López-Vilaró, Bell, Mora, Moral, Pérez, Arqueros, Ramón y Cajal, Lerma and Barnadas. |
|---|