Mechanism of Action of Streptococcus downii, a New Bacterial Species with Probiotic Potential

Streptococcus downii is a recently reported bacterial species of oral origin, with inhibitory capacity against Streptococcus mutans, Actinomyces naeslundii, Veillonella parvula and Aggregatibacter actinomycetemcomitans, which confers upon it the potential of being an oral probiotic. The aim of the p...

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Autores: Martínez Lamas, Lucía, García-Mato, E., Rincón Quintero, Anniris Maria, Rivas-Mundiña, B., Diz Dios, Pedro, Alvarez Fernández, Maximiliano
Tipo de recurso: artículo
Fecha de publicación:2023
País:España
Institución:Servizo Galego de Saúde (SERGAS)
Repositorio:RUNA. Repositorio da Consellería de Sanidade e Sergas
OAI Identifier:oai:runa.sergas.gal:20.500.11940/21730
Acceso en línea:https://portalcientifico.sergas.gal//documentos/6522c80aec1a10197ffd986a
http://hdl.handle.net/20.500.11940/21730
Access Level:acceso abierto
Palabra clave:AS Santiago
IDIS
AS Vigo
CHUVI
AS Santiago AP
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spelling Mechanism of Action of Streptococcus downii, a New Bacterial Species with Probiotic PotentialMartínez Lamas, LucíaGarcía-Mato, E.Rincón Quintero, Anniris MariaRivas-Mundiña, B.Diz Dios, PedroAlvarez Fernández, MaximilianoAS SantiagoIDISAS VigoCHUVIAS SantiagoAS Santiago APAS VigoCHUVIStreptococcus downii is a recently reported bacterial species of oral origin, with inhibitory capacity against Streptococcus mutans, Actinomyces naeslundii, Veillonella parvula and Aggregatibacter actinomycetemcomitans, which confers upon it the potential of being an oral probiotic. The aim of the present study was to identify the potential mechanisms by which S. downii exerts its inhibitory effect on S. mutans. To this end, the study assessed the consumption of glucose and proteins available in the culture medium, the modification of the pH, the production of short-chain fatty acids, the changes in the protein panel of the inhibition halo, the production of hydrogen peroxide and the effect of proteinase K. There were no differences in the glucose values or in the protein content of the medium, but there was a reduction in pH (with no effect on the growth of S. mutans). Significant increases were detected in the levels of lactic and formic acid (with no effect on the growth of S. mutans), as well as changes in the peptide panel (with no effect on the growth of S. mutans). The inhibitory effect was maintained in the presence of peroxidase but disappeared after adding proteinase K. Based on these results, it is suggested that the main mechanism of inhibition of S. downii against S. mutans is the production of bacteriocins.This research was funded by project IN855A-2016/36 financed by the Xunta de Galicia under the Resolution of the Galician Innovation Agency of 12 August 2016 (Ignicia Program); project I4TRANSF financed by the Galician Agency for Knowledge Management in Health (PRIS3 Program); project 00-00002474-16 financed by the Foundation for Innovation and Foresight in Health in Spain (FIPSE); and project DTS22/00056 funded by Instituto de Salud Carlos III (ISCIII) and co-funded by the European Union.2023info:eu-repo/semantics/articlehttps://portalcientifico.sergas.gal//documentos/6522c80aec1a10197ffd986ahttp://hdl.handle.net/20.500.11940/21730reponame:RUNA. Repositorio da Consellería de Sanidade e Sergasinstname:Servizo Galego de Saúde (SERGAS)Ingléshttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:runa.sergas.gal:20.500.11940/217302026-06-12T08:40:47Z
dc.title.none.fl_str_mv Mechanism of Action of Streptococcus downii, a New Bacterial Species with Probiotic Potential
title Mechanism of Action of Streptococcus downii, a New Bacterial Species with Probiotic Potential
spellingShingle Mechanism of Action of Streptococcus downii, a New Bacterial Species with Probiotic Potential
Martínez Lamas, Lucía
AS Santiago
IDIS
AS Vigo
CHUVI
AS Santiago
AS Santiago AP
AS Vigo
CHUVI
title_short Mechanism of Action of Streptococcus downii, a New Bacterial Species with Probiotic Potential
title_full Mechanism of Action of Streptococcus downii, a New Bacterial Species with Probiotic Potential
title_fullStr Mechanism of Action of Streptococcus downii, a New Bacterial Species with Probiotic Potential
title_full_unstemmed Mechanism of Action of Streptococcus downii, a New Bacterial Species with Probiotic Potential
title_sort Mechanism of Action of Streptococcus downii, a New Bacterial Species with Probiotic Potential
dc.creator.none.fl_str_mv Martínez Lamas, Lucía
García-Mato, E.
Rincón Quintero, Anniris Maria
Rivas-Mundiña, B.
Diz Dios, Pedro
Alvarez Fernández, Maximiliano
author Martínez Lamas, Lucía
author_facet Martínez Lamas, Lucía
García-Mato, E.
Rincón Quintero, Anniris Maria
Rivas-Mundiña, B.
Diz Dios, Pedro
Alvarez Fernández, Maximiliano
author_role author
author2 García-Mato, E.
Rincón Quintero, Anniris Maria
Rivas-Mundiña, B.
Diz Dios, Pedro
Alvarez Fernández, Maximiliano
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv AS Santiago
IDIS
AS Vigo
CHUVI
AS Santiago
AS Santiago AP
AS Vigo
CHUVI
topic AS Santiago
IDIS
AS Vigo
CHUVI
AS Santiago
AS Santiago AP
AS Vigo
CHUVI
description Streptococcus downii is a recently reported bacterial species of oral origin, with inhibitory capacity against Streptococcus mutans, Actinomyces naeslundii, Veillonella parvula and Aggregatibacter actinomycetemcomitans, which confers upon it the potential of being an oral probiotic. The aim of the present study was to identify the potential mechanisms by which S. downii exerts its inhibitory effect on S. mutans. To this end, the study assessed the consumption of glucose and proteins available in the culture medium, the modification of the pH, the production of short-chain fatty acids, the changes in the protein panel of the inhibition halo, the production of hydrogen peroxide and the effect of proteinase K. There were no differences in the glucose values or in the protein content of the medium, but there was a reduction in pH (with no effect on the growth of S. mutans). Significant increases were detected in the levels of lactic and formic acid (with no effect on the growth of S. mutans), as well as changes in the peptide panel (with no effect on the growth of S. mutans). The inhibitory effect was maintained in the presence of peroxidase but disappeared after adding proteinase K. Based on these results, it is suggested that the main mechanism of inhibition of S. downii against S. mutans is the production of bacteriocins.
publishDate 2023
dc.date.none.fl_str_mv 2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://portalcientifico.sergas.gal//documentos/6522c80aec1a10197ffd986a
http://hdl.handle.net/20.500.11940/21730
url https://portalcientifico.sergas.gal//documentos/6522c80aec1a10197ffd986a
http://hdl.handle.net/20.500.11940/21730
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:RUNA. Repositorio da Consellería de Sanidade e Sergas
instname:Servizo Galego de Saúde (SERGAS)
instname_str Servizo Galego de Saúde (SERGAS)
reponame_str RUNA. Repositorio da Consellería de Sanidade e Sergas
collection RUNA. Repositorio da Consellería de Sanidade e Sergas
repository.name.fl_str_mv
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