Marinobacter azerbaijanicus sp. nov., a moderately halophilic bacterium from Urmia Lake, Iran

A novel moderately halophilic, Gram-stain-negative and facultatively anaerobic bacterium, designated as strain TBZ242T, was isolated from water of Urmia Lake in the Azerbaijan region of Iran. The cells were found to be rod-shaped and motile by a single polar flagellum, producing circular and yellowi...

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Autores: Sánchez-Porro Álvarez, Cristina, Aghdam, Elnaz Mehdizadeh, Montazersaheb, Soheila, Tarhriz, Vahideh, Kazemi, Elham, Amoozegar, Mohammad Ali, Ventosa Ucero, Antonio, Hejazi, Mohammad Saeid
Formato: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2024
País:España
Recursos:Universidad de Sevilla (US)
Repositorio:idUS. Depósito de Investigación de la Universidad de Sevilla
OAI Identifier:oai:idus.us.es:11441/164086
Acesso em linha:https://hdl.handle.net/11441/164086
https://doi.org/10.1099/ijsem.0.006308
Access Level:acceso abierto
Palavra-chave:Comparative genomics
Halophilic bacteria
Marinobacter
New taxa
Taxonomy
Urmia Lake
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spelling Marinobacter azerbaijanicus sp. nov., a moderately halophilic bacterium from Urmia Lake, IranSánchez-Porro Álvarez, CristinaAghdam, Elnaz MehdizadehMontazersaheb, SoheilaTarhriz, VahidehKazemi, ElhamAmoozegar, Mohammad AliVentosa Ucero, AntonioHejazi, Mohammad SaeidComparative genomicsHalophilic bacteriaMarinobacterNew taxaTaxonomyUrmia LakeA novel moderately halophilic, Gram-stain-negative and facultatively anaerobic bacterium, designated as strain TBZ242T, was isolated from water of Urmia Lake in the Azerbaijan region of Iran. The cells were found to be rod-shaped and motile by a single polar flagellum, producing circular and yellowish colonies. The strain could grow in the presence of 0.5–10 % (w/v) NaCl (optimum, 2.5–5 %). The temperature and pH ranges for growth were 15–45 °C (optimum 30 °C) and pH 7.0–11.0 (optimum pH 8.0) on marine agar. The 16S rRNA gene sequence analysis revealed that strain TBZ242T belonged to the genus Marinobacter, showing the highest similarities to Marinobacter algicola DG893T (98.8 %), Marinobacter vulgaris F01T (98.8 %), Marinobacter salarius R9SW1T (98.5 %), Marinobacter panjinensis PJ-16T (98.4 %), Marinobacter orientalis W62T (98.0 %) and Marinobacter denitrificans JB2H27T (98.0 %). The 16S rRNA and core-genome phylogenetic trees showed that strain TBZ242T formed a distinct branch, closely related to a subclade accommodating M. vulgaris, M. orientalis, M. panjinensis, M. denitrificans, M. algicola, M. salarius and M. iranensis, within the genus Marinobacter. Average nucleotide identity and digital DNA–DNA hybridization values between strain TBZ242T and the type strains of the related species of Marinobacter were ≤85.0 and 28.6 %, respectively, confirming that strain TBZ242T represents a distinct species. The major cellular fatty acids of strain TBZ242T were C16: 0 and C16: 1 ω7c/C16: 1 ω6c and the quinone was ubiquinone Q-9. The genomic DNA G+C content of strain TBZ242T is 57.2 mol%. Based on phenotypic, chemotaxonomic and genomic data, strain TBZ242T represents a novel species within the genus Marinobacter, for which the name Marinobacter azerbaijanicus sp. nov. is proposed. The type strain is TBZ242T (= CECT 30649T = IBRC-M 11466T). Genomic fragment recruitment analysis showed that this species prefers aquatic saline environments with intermediate salinities, being detected on metagenomic databases of Lake Meyghan (Iran) with 5 and 18 % salinity, respectively.University of Medical Sciences, Tabriz, Iran IR.TBZMED.VCR.REC.1400.253Ministerio de Ciencia e Innovación PID2020-118136GB-I00Junta de Andalucía P20_01066, BIO-213Microbiology SocietyMicrobiología y ParasitologíaUniversity of Medical Sciences, Tabriz, IranMinisterio de Ciencia e Innovación (MICIN). EspañaJunta de Andalucía2024info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionapplication/pdfapplication/pdfhttps://hdl.handle.net/11441/164086https://doi.org/10.1099/ijsem.0.006308reponame:idUS. Depósito de Investigación de la Universidad de Sevillainstname:Universidad de Sevilla (US)InglésInternational Journal of Systematic and Evolutionary Microbiology, 74 (4), 006308.IR.TBZMED.VCR.REC.1400.253PID2020-118136GB-I00P20_01066BIO-213https://doi.org/10.1099/ijsem.0.006308info:eu-repo/semantics/openAccessoai:idus.us.es:11441/1640862026-06-17T12:51:07Z
dc.title.none.fl_str_mv Marinobacter azerbaijanicus sp. nov., a moderately halophilic bacterium from Urmia Lake, Iran
title Marinobacter azerbaijanicus sp. nov., a moderately halophilic bacterium from Urmia Lake, Iran
spellingShingle Marinobacter azerbaijanicus sp. nov., a moderately halophilic bacterium from Urmia Lake, Iran
Sánchez-Porro Álvarez, Cristina
Comparative genomics
Halophilic bacteria
Marinobacter
New taxa
Taxonomy
Urmia Lake
title_short Marinobacter azerbaijanicus sp. nov., a moderately halophilic bacterium from Urmia Lake, Iran
title_full Marinobacter azerbaijanicus sp. nov., a moderately halophilic bacterium from Urmia Lake, Iran
title_fullStr Marinobacter azerbaijanicus sp. nov., a moderately halophilic bacterium from Urmia Lake, Iran
title_full_unstemmed Marinobacter azerbaijanicus sp. nov., a moderately halophilic bacterium from Urmia Lake, Iran
title_sort Marinobacter azerbaijanicus sp. nov., a moderately halophilic bacterium from Urmia Lake, Iran
dc.creator.none.fl_str_mv Sánchez-Porro Álvarez, Cristina
Aghdam, Elnaz Mehdizadeh
Montazersaheb, Soheila
Tarhriz, Vahideh
Kazemi, Elham
Amoozegar, Mohammad Ali
Ventosa Ucero, Antonio
Hejazi, Mohammad Saeid
author Sánchez-Porro Álvarez, Cristina
author_facet Sánchez-Porro Álvarez, Cristina
Aghdam, Elnaz Mehdizadeh
Montazersaheb, Soheila
Tarhriz, Vahideh
Kazemi, Elham
Amoozegar, Mohammad Ali
Ventosa Ucero, Antonio
Hejazi, Mohammad Saeid
author_role author
author2 Aghdam, Elnaz Mehdizadeh
Montazersaheb, Soheila
Tarhriz, Vahideh
Kazemi, Elham
Amoozegar, Mohammad Ali
Ventosa Ucero, Antonio
Hejazi, Mohammad Saeid
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Microbiología y Parasitología
University of Medical Sciences, Tabriz, Iran
Ministerio de Ciencia e Innovación (MICIN). España
Junta de Andalucía
dc.subject.none.fl_str_mv Comparative genomics
Halophilic bacteria
Marinobacter
New taxa
Taxonomy
Urmia Lake
topic Comparative genomics
Halophilic bacteria
Marinobacter
New taxa
Taxonomy
Urmia Lake
description A novel moderately halophilic, Gram-stain-negative and facultatively anaerobic bacterium, designated as strain TBZ242T, was isolated from water of Urmia Lake in the Azerbaijan region of Iran. The cells were found to be rod-shaped and motile by a single polar flagellum, producing circular and yellowish colonies. The strain could grow in the presence of 0.5–10 % (w/v) NaCl (optimum, 2.5–5 %). The temperature and pH ranges for growth were 15–45 °C (optimum 30 °C) and pH 7.0–11.0 (optimum pH 8.0) on marine agar. The 16S rRNA gene sequence analysis revealed that strain TBZ242T belonged to the genus Marinobacter, showing the highest similarities to Marinobacter algicola DG893T (98.8 %), Marinobacter vulgaris F01T (98.8 %), Marinobacter salarius R9SW1T (98.5 %), Marinobacter panjinensis PJ-16T (98.4 %), Marinobacter orientalis W62T (98.0 %) and Marinobacter denitrificans JB2H27T (98.0 %). The 16S rRNA and core-genome phylogenetic trees showed that strain TBZ242T formed a distinct branch, closely related to a subclade accommodating M. vulgaris, M. orientalis, M. panjinensis, M. denitrificans, M. algicola, M. salarius and M. iranensis, within the genus Marinobacter. Average nucleotide identity and digital DNA–DNA hybridization values between strain TBZ242T and the type strains of the related species of Marinobacter were ≤85.0 and 28.6 %, respectively, confirming that strain TBZ242T represents a distinct species. The major cellular fatty acids of strain TBZ242T were C16: 0 and C16: 1 ω7c/C16: 1 ω6c and the quinone was ubiquinone Q-9. The genomic DNA G+C content of strain TBZ242T is 57.2 mol%. Based on phenotypic, chemotaxonomic and genomic data, strain TBZ242T represents a novel species within the genus Marinobacter, for which the name Marinobacter azerbaijanicus sp. nov. is proposed. The type strain is TBZ242T (= CECT 30649T = IBRC-M 11466T). Genomic fragment recruitment analysis showed that this species prefers aquatic saline environments with intermediate salinities, being detected on metagenomic databases of Lake Meyghan (Iran) with 5 and 18 % salinity, respectively.
publishDate 2024
dc.date.none.fl_str_mv 2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/11441/164086
https://doi.org/10.1099/ijsem.0.006308
url https://hdl.handle.net/11441/164086
https://doi.org/10.1099/ijsem.0.006308
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv International Journal of Systematic and Evolutionary Microbiology, 74 (4), 006308.
IR.TBZMED.VCR.REC.1400.253
PID2020-118136GB-I00
P20_01066
BIO-213
https://doi.org/10.1099/ijsem.0.006308
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Microbiology Society
publisher.none.fl_str_mv Microbiology Society
dc.source.none.fl_str_mv reponame:idUS. Depósito de Investigación de la Universidad de Sevilla
instname:Universidad de Sevilla (US)
instname_str Universidad de Sevilla (US)
reponame_str idUS. Depósito de Investigación de la Universidad de Sevilla
collection idUS. Depósito de Investigación de la Universidad de Sevilla
repository.name.fl_str_mv
repository.mail.fl_str_mv
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