Establishment of cryopreserved gene banks of European chestnut and cork oak
Cryopreservation of selected genotypes of Europeanchestnut and cork oak was carried out in two laboratories in a proect involving conservation of field collections. Plant material was selected on the basis of disease resistance (chestnut), growth habit, phytosanitary performance and cork quality (co...
| Autores: | , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Fecha de publicación: | 2010 |
| País: | España |
| Institución: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/22592 |
| Acceso en línea: | http://hdl.handle.net/10261/22592 |
| Access Level: | acceso abierto |
| Palabra clave: | Castanea sativa Cryopreservation Forest tree species Liquid nitrogen Quercus suber Vitrification |
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Establishment of cryopreserved gene banks of European chestnut and cork oak Vidal González, Nieves PilarViéitez Martín, Ana MaríaFernández, Rosario M.Cuenca, BeatrizBallester, AntonioCastanea sativaCryopreservationForest tree speciesLiquid nitrogenQuercus suberVitrificationCryopreservation of selected genotypes of Europeanchestnut and cork oak was carried out in two laboratories in a proect involving conservation of field collections. Plant material was selected on the basis of disease resistance (chestnut), growth habit, phytosanitary performance and cork quality (cork oak). The cryopreservation technique comprised vitrification of shoot apices isolated from in vitro stock shoot cultures (chestnut) and somatic embryos (cork oak). Forty three out 46 chestnut genotypes assayed survived the freezing process, but only 63% recovered their capacity to produce new shoots. After completion of multiplication and rooting steps, the surviving shoots produced plants that were morphologically identical to those derived from non-supercooled material. All 51 cork oak genotypes withstood freezing and were able to produce new somatic embryos through a process of secondary embrygenesis. Multiplication and germination of the recovered embryos enabled production of plants that were morphologically identical to thse derived from non‐supercooled material. In light of the results obtained, long-term cryopreservation of these species is feasible, thereby ensuring conservation of valuable genotypes during field Peer reviewed201020102010info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_65011026917 bytesapplication/pdfhttp://hdl.handle.net/10261/22592reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglésinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/225922026-05-22T06:33:51Z |
| dc.title.none.fl_str_mv |
Establishment of cryopreserved gene banks of European chestnut and cork oak |
| title |
Establishment of cryopreserved gene banks of European chestnut and cork oak |
| spellingShingle |
Establishment of cryopreserved gene banks of European chestnut and cork oak Vidal González, Nieves Pilar Castanea sativa Cryopreservation Forest tree species Liquid nitrogen Quercus suber Vitrification |
| title_short |
Establishment of cryopreserved gene banks of European chestnut and cork oak |
| title_full |
Establishment of cryopreserved gene banks of European chestnut and cork oak |
| title_fullStr |
Establishment of cryopreserved gene banks of European chestnut and cork oak |
| title_full_unstemmed |
Establishment of cryopreserved gene banks of European chestnut and cork oak |
| title_sort |
Establishment of cryopreserved gene banks of European chestnut and cork oak |
| dc.creator.none.fl_str_mv |
Vidal González, Nieves Pilar Viéitez Martín, Ana María Fernández, Rosario M. Cuenca, Beatriz Ballester, Antonio |
| author |
Vidal González, Nieves Pilar |
| author_facet |
Vidal González, Nieves Pilar Viéitez Martín, Ana María Fernández, Rosario M. Cuenca, Beatriz Ballester, Antonio |
| author_role |
author |
| author2 |
Viéitez Martín, Ana María Fernández, Rosario M. Cuenca, Beatriz Ballester, Antonio |
| author2_role |
author author author author |
| dc.subject.none.fl_str_mv |
Castanea sativa Cryopreservation Forest tree species Liquid nitrogen Quercus suber Vitrification |
| topic |
Castanea sativa Cryopreservation Forest tree species Liquid nitrogen Quercus suber Vitrification |
| description |
Cryopreservation of selected genotypes of Europeanchestnut and cork oak was carried out in two laboratories in a proect involving conservation of field collections. Plant material was selected on the basis of disease resistance (chestnut), growth habit, phytosanitary performance and cork quality (cork oak). The cryopreservation technique comprised vitrification of shoot apices isolated from in vitro stock shoot cultures (chestnut) and somatic embryos (cork oak). Forty three out 46 chestnut genotypes assayed survived the freezing process, but only 63% recovered their capacity to produce new shoots. After completion of multiplication and rooting steps, the surviving shoots produced plants that were morphologically identical to those derived from non-supercooled material. All 51 cork oak genotypes withstood freezing and were able to produce new somatic embryos through a process of secondary embrygenesis. Multiplication and germination of the recovered embryos enabled production of plants that were morphologically identical to thse derived from non‐supercooled material. In light of the results obtained, long-term cryopreservation of these species is feasible, thereby ensuring conservation of valuable genotypes during field |
| publishDate |
2010 |
| dc.date.none.fl_str_mv |
2010 2010 2010 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article http://purl.org/coar/resource_type/c_6501 |
| format |
article |
| dc.identifier.none.fl_str_mv |
http://hdl.handle.net/10261/22592 |
| url |
http://hdl.handle.net/10261/22592 |
| dc.language.none.fl_str_mv |
Inglés |
| language_invalid_str_mv |
Inglés |
| dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess |
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openAccess |
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1026917 bytes application/pdf |
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Consejo Superior de Investigaciones Científicas (CSIC) |
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DIGITAL.CSIC. Repositorio Institucional del CSIC |
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DIGITAL.CSIC. Repositorio Institucional del CSIC |
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15,812429 |